Thymidine phosphorylase activity associated with platelet-derived endothelial cell growth factor

Tomoyuki Sumizawa, Tatsuhiko Furukawa, Misako Haraguchi, Akihiko Yoshimura, Akira Takeyasu, Minoru Ishizawa, Yuji Yamada, Shin Ichi Akiyama

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

Partial complementary DNA (cDNA) for thymidine phosphorylase (dThdPase) was cloned by means of a polymerase chain reaction. There was complete sequence identity between the amino acid sequence deduced from the nucleotide sequence of a clone (288 nucleotides) and the residues of platelet-derived endothelial cell growth factor (PD-ECGF). The amino acid sequence of all four peptide fragments from purified human dThdPase could be aligned with that of PD-ECGF. Our data indicate that residues 125-244 of PD-ECGF are identical to the sequence of human dThdPase. The molecular weights of human dThdPase and recombinant PD-ECGF (rPD-ECGF) that lacks 10 amino acids at the amino terminal were 55 and 52 kDa, respectively. Anti-PD-ECGF antibody recognized dThdPase, and anti-dThdPase antibody recognized rPD-ECGF. rPD-ECGF had dThdPase activity and its specific activity was similar to that of purified human dThdPase. dThdPase activity and molecules were detected in COS cells transfected with human PD-ECGF cDNA, but not in nontransfected cells. The sizes of PD-ECGF and dThdPase in the transfected COS cells were identical. These data suggest that human dThdPase is identical to PD-ECGF.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalJournal of Biochemistry
Volume114
Issue number1
Publication statusPublished - 1993 Jul
Externally publishedYes

Fingerprint

Thymidine Phosphorylase
Endothelial Cells
Platelets
Growth Factors
Endothelial cells
Cell growth
CDNA
Complementary DNA
Amino acids
Nucleotides
Antibodies
Amino Acid Sequence
DNA
Antibody
Cell
Intercellular Signaling Peptides and Proteins
cDNA
Growth factors
Polymerase chain reaction
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Sumizawa, T., Furukawa, T., Haraguchi, M., Yoshimura, A., Takeyasu, A., Ishizawa, M., ... Akiyama, S. I. (1993). Thymidine phosphorylase activity associated with platelet-derived endothelial cell growth factor. Journal of Biochemistry, 114(1), 9-14.

Thymidine phosphorylase activity associated with platelet-derived endothelial cell growth factor. / Sumizawa, Tomoyuki; Furukawa, Tatsuhiko; Haraguchi, Misako; Yoshimura, Akihiko; Takeyasu, Akira; Ishizawa, Minoru; Yamada, Yuji; Akiyama, Shin Ichi.

In: Journal of Biochemistry, Vol. 114, No. 1, 07.1993, p. 9-14.

Research output: Contribution to journalArticle

Sumizawa, T, Furukawa, T, Haraguchi, M, Yoshimura, A, Takeyasu, A, Ishizawa, M, Yamada, Y & Akiyama, SI 1993, 'Thymidine phosphorylase activity associated with platelet-derived endothelial cell growth factor', Journal of Biochemistry, vol. 114, no. 1, pp. 9-14.
Sumizawa T, Furukawa T, Haraguchi M, Yoshimura A, Takeyasu A, Ishizawa M et al. Thymidine phosphorylase activity associated with platelet-derived endothelial cell growth factor. Journal of Biochemistry. 1993 Jul;114(1):9-14.
Sumizawa, Tomoyuki ; Furukawa, Tatsuhiko ; Haraguchi, Misako ; Yoshimura, Akihiko ; Takeyasu, Akira ; Ishizawa, Minoru ; Yamada, Yuji ; Akiyama, Shin Ichi. / Thymidine phosphorylase activity associated with platelet-derived endothelial cell growth factor. In: Journal of Biochemistry. 1993 ; Vol. 114, No. 1. pp. 9-14.
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