Thyroid hormone regulates reelin and dab1 expression during brain development

Manuel Alvarez-Dolado, Mónica Ruiz, José A. Del Río, Soledad Alcántara, Ferran Burgaya, Michael Sheldon, Kazunori Nakajima, Juan Bernal, Brian W. Howell, Tom Curran, Eduardo Soriano, Alberto Muñoz

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

The reelin and dab1 genes are necessary for appropriate neuronal migration and lamination during brain development. Since these processes are controlled by thyroid hormone, we studied the effect of thyroid hormone deprivation and administration on the expression of reelin and dab1. As shown by Northern analysis, in situ hybridization, and immunohistochemistry studies, hypothyroid rats expressed decreased levels of reelin RNA and protein during the perinatal period [embryonic day 18 (E18) and postnatal day 0 (P0)]. The effect was evident in Cajal-Retzius cells of cortex layer I, as well as in layers V/VI, hippocampus, and granular neurons of the cerebellum. At later ages, however, Reelin was more abundant in the cortex, hippocampus, cerebellum, and olfactory bulb of hypothyroid rats (P5), and no differences were detected at P15. Conversely, Dab1 levels were higher at P0, and lower at P5 in hypothyroid animals. In line with these results, reelin RNA and protein levels were higher in cultured hippocampal slices from P0 control rats compared to those from hypothyroid animals. Significantly, thyroid-dependent regulation of reelin and dab1 was confirmed in vivo and in vitro by hormone treatment of hypothyroid rats and organotypic cultures, respectively. In both cases, thyroid hormone led to an increase in reelin expression. Our data suggest that the effects of thyroid hormone on neuronal migration may be in part mediated through the control of reelin and dab1 expression during brain ontogenesis.

Original languageEnglish
Pages (from-to)6979-6993
Number of pages15
JournalJournal of Neuroscience
Volume19
Issue number16
Publication statusPublished - 1999 Aug 15
Externally publishedYes

Fingerprint

Thyroid Hormones
Brain
Cerebellum
Hippocampus
RNA
Olfactory Bulb
In Situ Hybridization
Thyroid Gland
Immunohistochemistry
Hormones
Neurons
Genes
reelin protein
Therapeutics

Keywords

  • Brain development
  • Cortical lamination
  • dab1
  • Neuronal migration
  • Reelin
  • Thyroid hormone

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Alvarez-Dolado, M., Ruiz, M., Del Río, J. A., Alcántara, S., Burgaya, F., Sheldon, M., ... Muñoz, A. (1999). Thyroid hormone regulates reelin and dab1 expression during brain development. Journal of Neuroscience, 19(16), 6979-6993.

Thyroid hormone regulates reelin and dab1 expression during brain development. / Alvarez-Dolado, Manuel; Ruiz, Mónica; Del Río, José A.; Alcántara, Soledad; Burgaya, Ferran; Sheldon, Michael; Nakajima, Kazunori; Bernal, Juan; Howell, Brian W.; Curran, Tom; Soriano, Eduardo; Muñoz, Alberto.

In: Journal of Neuroscience, Vol. 19, No. 16, 15.08.1999, p. 6979-6993.

Research output: Contribution to journalArticle

Alvarez-Dolado, M, Ruiz, M, Del Río, JA, Alcántara, S, Burgaya, F, Sheldon, M, Nakajima, K, Bernal, J, Howell, BW, Curran, T, Soriano, E & Muñoz, A 1999, 'Thyroid hormone regulates reelin and dab1 expression during brain development', Journal of Neuroscience, vol. 19, no. 16, pp. 6979-6993.
Alvarez-Dolado M, Ruiz M, Del Río JA, Alcántara S, Burgaya F, Sheldon M et al. Thyroid hormone regulates reelin and dab1 expression during brain development. Journal of Neuroscience. 1999 Aug 15;19(16):6979-6993.
Alvarez-Dolado, Manuel ; Ruiz, Mónica ; Del Río, José A. ; Alcántara, Soledad ; Burgaya, Ferran ; Sheldon, Michael ; Nakajima, Kazunori ; Bernal, Juan ; Howell, Brian W. ; Curran, Tom ; Soriano, Eduardo ; Muñoz, Alberto. / Thyroid hormone regulates reelin and dab1 expression during brain development. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 16. pp. 6979-6993.
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