Thyroid hormone stimulates renin synthesis in rats without involving the sympathetic nervous system

Hiroyuki Kobori, Atsuhiro Ichihara, Hiromichi Suzuki, Yutaka Miyashita, Matsuhiko Hayashi, Takao Saruta

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The present study was performed to examine whether renal expression of the renin gene is regulated by thyroid hormone. Thirty male Sprague-Dawley rats were divided into hypothyroid, control, and hyperthyroid groups by use of daily intraperitoneal administration of methimazole, saline vehicle, or thyroxine, respectively. Each group was further subdivided into sympathetic innervated and sympathetic denervated subgroups by use of intraperitoneal administration of saline vehicle or 6-hydroxydopamine. Plasma renin activity and renal levels of renin were measured by radioimmunoassays after 8 wk. Renal expression of renin mRNA was evaluated by a semiquantitative reverse transcriptase-polymerase chain reaction. Compared with control animals, plasma renin activity, renal level of renin, and renal expression of renin mRNA were reduced (82, 94, and 71%, respectively) in hypothyroid animals and elevated (155, 1,182, and 152%, respectively) in hyperthyroid animals. Sympathetic denervation had no independent effect on these renin values. Our results indicate that thyroid hormone stimulates renin synthesis without involving the sympathetic nervous system.

Original languageEnglish
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume272
Issue number2 35-2
Publication statusPublished - 1997 Feb

Fingerprint

Sympathetic Nervous System
Neurology
Thyroid Hormones
Renin
Rats
Kidney
Animals
Hyperthyroidism
Methimazole
Plasmas
Messenger RNA
Sympathectomy
Oxidopamine
Polymerase chain reaction
RNA-Directed DNA Polymerase
Reverse Transcriptase Polymerase Chain Reaction
Thyroxine
Radioimmunoassay
Sprague Dawley Rats
Genes

Keywords

  • chemical sympathectomy
  • polymerase chain reaction
  • renin-angiotensin system

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Thyroid hormone stimulates renin synthesis in rats without involving the sympathetic nervous system. / Kobori, Hiroyuki; Ichihara, Atsuhiro; Suzuki, Hiromichi; Miyashita, Yutaka; Hayashi, Matsuhiko; Saruta, Takao.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 272, No. 2 35-2, 02.1997.

Research output: Contribution to journalArticle

Kobori, Hiroyuki ; Ichihara, Atsuhiro ; Suzuki, Hiromichi ; Miyashita, Yutaka ; Hayashi, Matsuhiko ; Saruta, Takao. / Thyroid hormone stimulates renin synthesis in rats without involving the sympathetic nervous system. In: American Journal of Physiology - Endocrinology and Metabolism. 1997 ; Vol. 272, No. 2 35-2.
@article{da63c0b57eff4452babfe455f6ba452b,
title = "Thyroid hormone stimulates renin synthesis in rats without involving the sympathetic nervous system",
abstract = "The present study was performed to examine whether renal expression of the renin gene is regulated by thyroid hormone. Thirty male Sprague-Dawley rats were divided into hypothyroid, control, and hyperthyroid groups by use of daily intraperitoneal administration of methimazole, saline vehicle, or thyroxine, respectively. Each group was further subdivided into sympathetic innervated and sympathetic denervated subgroups by use of intraperitoneal administration of saline vehicle or 6-hydroxydopamine. Plasma renin activity and renal levels of renin were measured by radioimmunoassays after 8 wk. Renal expression of renin mRNA was evaluated by a semiquantitative reverse transcriptase-polymerase chain reaction. Compared with control animals, plasma renin activity, renal level of renin, and renal expression of renin mRNA were reduced (82, 94, and 71{\%}, respectively) in hypothyroid animals and elevated (155, 1,182, and 152{\%}, respectively) in hyperthyroid animals. Sympathetic denervation had no independent effect on these renin values. Our results indicate that thyroid hormone stimulates renin synthesis without involving the sympathetic nervous system.",
keywords = "chemical sympathectomy, polymerase chain reaction, renin-angiotensin system",
author = "Hiroyuki Kobori and Atsuhiro Ichihara and Hiromichi Suzuki and Yutaka Miyashita and Matsuhiko Hayashi and Takao Saruta",
year = "1997",
month = "2",
language = "English",
volume = "272",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "2 35-2",

}

TY - JOUR

T1 - Thyroid hormone stimulates renin synthesis in rats without involving the sympathetic nervous system

AU - Kobori, Hiroyuki

AU - Ichihara, Atsuhiro

AU - Suzuki, Hiromichi

AU - Miyashita, Yutaka

AU - Hayashi, Matsuhiko

AU - Saruta, Takao

PY - 1997/2

Y1 - 1997/2

N2 - The present study was performed to examine whether renal expression of the renin gene is regulated by thyroid hormone. Thirty male Sprague-Dawley rats were divided into hypothyroid, control, and hyperthyroid groups by use of daily intraperitoneal administration of methimazole, saline vehicle, or thyroxine, respectively. Each group was further subdivided into sympathetic innervated and sympathetic denervated subgroups by use of intraperitoneal administration of saline vehicle or 6-hydroxydopamine. Plasma renin activity and renal levels of renin were measured by radioimmunoassays after 8 wk. Renal expression of renin mRNA was evaluated by a semiquantitative reverse transcriptase-polymerase chain reaction. Compared with control animals, plasma renin activity, renal level of renin, and renal expression of renin mRNA were reduced (82, 94, and 71%, respectively) in hypothyroid animals and elevated (155, 1,182, and 152%, respectively) in hyperthyroid animals. Sympathetic denervation had no independent effect on these renin values. Our results indicate that thyroid hormone stimulates renin synthesis without involving the sympathetic nervous system.

AB - The present study was performed to examine whether renal expression of the renin gene is regulated by thyroid hormone. Thirty male Sprague-Dawley rats were divided into hypothyroid, control, and hyperthyroid groups by use of daily intraperitoneal administration of methimazole, saline vehicle, or thyroxine, respectively. Each group was further subdivided into sympathetic innervated and sympathetic denervated subgroups by use of intraperitoneal administration of saline vehicle or 6-hydroxydopamine. Plasma renin activity and renal levels of renin were measured by radioimmunoassays after 8 wk. Renal expression of renin mRNA was evaluated by a semiquantitative reverse transcriptase-polymerase chain reaction. Compared with control animals, plasma renin activity, renal level of renin, and renal expression of renin mRNA were reduced (82, 94, and 71%, respectively) in hypothyroid animals and elevated (155, 1,182, and 152%, respectively) in hyperthyroid animals. Sympathetic denervation had no independent effect on these renin values. Our results indicate that thyroid hormone stimulates renin synthesis without involving the sympathetic nervous system.

KW - chemical sympathectomy

KW - polymerase chain reaction

KW - renin-angiotensin system

UR - http://www.scopus.com/inward/record.url?scp=0030893713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030893713&partnerID=8YFLogxK

M3 - Article

C2 - 9124327

AN - SCOPUS:0030893713

VL - 272

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 2 35-2

ER -