Tight-binding inhibitory sequences against pp60(c-src) identified using a random 15-amino-acid peptide library

Toru Nishi, Raymond J A Budde, John S. McMurray, Nihal U. Obeyesekere, Naueen Safdar, Victor A. Levin, Hideyuki Saya

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A bacteriophage peptide library containing a random 15-amino-acid insert was screened for identification of peptide sequence(s) that bind pp60(c-src). Sequencing the random insert from more than 100 virions indicated that more than 60% of the phage virions that bound to this enzyme contained a GXXG sequence motif in which X was frequently a hydrophobic residue. The GXXG sequence was often repeated as GXXGXXG. Two nonameric peptides were synthesized to determine whether or not the peptide inhibits pp60(c-src) tyrosine kinase activity and the importance of the glycine residues within this sequence. The peptide containing glycine had a K(i) of 24 μM, whereas replacing the glycines with proline increased the K(i) value to 3.1 mM.

Original languageEnglish
Pages (from-to)237-240
Number of pages4
JournalFEBS Letters
Volume399
Issue number3
DOIs
Publication statusPublished - 1996 Dec 16
Externally publishedYes

Fingerprint

Proto-Oncogene Proteins pp60(c-src)
Peptide Library
Glycine
Amino Acids
Peptides
Bacteriophages
Virion
Proline
Enzymes

Keywords

  • Peptide inhibitor
  • pp60(c-src)
  • Random phage library

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Tight-binding inhibitory sequences against pp60(c-src) identified using a random 15-amino-acid peptide library. / Nishi, Toru; Budde, Raymond J A; McMurray, John S.; Obeyesekere, Nihal U.; Safdar, Naueen; Levin, Victor A.; Saya, Hideyuki.

In: FEBS Letters, Vol. 399, No. 3, 16.12.1996, p. 237-240.

Research output: Contribution to journalArticle

Nishi, Toru ; Budde, Raymond J A ; McMurray, John S. ; Obeyesekere, Nihal U. ; Safdar, Naueen ; Levin, Victor A. ; Saya, Hideyuki. / Tight-binding inhibitory sequences against pp60(c-src) identified using a random 15-amino-acid peptide library. In: FEBS Letters. 1996 ; Vol. 399, No. 3. pp. 237-240.
@article{dd69c9bd12a344d4b9fab833c2ee527f,
title = "Tight-binding inhibitory sequences against pp60(c-src) identified using a random 15-amino-acid peptide library",
abstract = "A bacteriophage peptide library containing a random 15-amino-acid insert was screened for identification of peptide sequence(s) that bind pp60(c-src). Sequencing the random insert from more than 100 virions indicated that more than 60{\%} of the phage virions that bound to this enzyme contained a GXXG sequence motif in which X was frequently a hydrophobic residue. The GXXG sequence was often repeated as GXXGXXG. Two nonameric peptides were synthesized to determine whether or not the peptide inhibits pp60(c-src) tyrosine kinase activity and the importance of the glycine residues within this sequence. The peptide containing glycine had a K(i) of 24 μM, whereas replacing the glycines with proline increased the K(i) value to 3.1 mM.",
keywords = "Peptide inhibitor, pp60(c-src), Random phage library",
author = "Toru Nishi and Budde, {Raymond J A} and McMurray, {John S.} and Obeyesekere, {Nihal U.} and Naueen Safdar and Levin, {Victor A.} and Hideyuki Saya",
year = "1996",
month = "12",
day = "16",
doi = "10.1016/S0014-5793(96)01329-4",
language = "English",
volume = "399",
pages = "237--240",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Tight-binding inhibitory sequences against pp60(c-src) identified using a random 15-amino-acid peptide library

AU - Nishi, Toru

AU - Budde, Raymond J A

AU - McMurray, John S.

AU - Obeyesekere, Nihal U.

AU - Safdar, Naueen

AU - Levin, Victor A.

AU - Saya, Hideyuki

PY - 1996/12/16

Y1 - 1996/12/16

N2 - A bacteriophage peptide library containing a random 15-amino-acid insert was screened for identification of peptide sequence(s) that bind pp60(c-src). Sequencing the random insert from more than 100 virions indicated that more than 60% of the phage virions that bound to this enzyme contained a GXXG sequence motif in which X was frequently a hydrophobic residue. The GXXG sequence was often repeated as GXXGXXG. Two nonameric peptides were synthesized to determine whether or not the peptide inhibits pp60(c-src) tyrosine kinase activity and the importance of the glycine residues within this sequence. The peptide containing glycine had a K(i) of 24 μM, whereas replacing the glycines with proline increased the K(i) value to 3.1 mM.

AB - A bacteriophage peptide library containing a random 15-amino-acid insert was screened for identification of peptide sequence(s) that bind pp60(c-src). Sequencing the random insert from more than 100 virions indicated that more than 60% of the phage virions that bound to this enzyme contained a GXXG sequence motif in which X was frequently a hydrophobic residue. The GXXG sequence was often repeated as GXXGXXG. Two nonameric peptides were synthesized to determine whether or not the peptide inhibits pp60(c-src) tyrosine kinase activity and the importance of the glycine residues within this sequence. The peptide containing glycine had a K(i) of 24 μM, whereas replacing the glycines with proline increased the K(i) value to 3.1 mM.

KW - Peptide inhibitor

KW - pp60(c-src)

KW - Random phage library

UR - http://www.scopus.com/inward/record.url?scp=0030590839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030590839&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(96)01329-4

DO - 10.1016/S0014-5793(96)01329-4

M3 - Article

VL - 399

SP - 237

EP - 240

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -