Time dependence of myocardial cell necrosis during photodynamic therapy with various photosensitizer contact time

Emiyu Ogawa, Yuiko Kikuchi, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We studied how the photosensitizer contact time affects the myocardial necrosis time during photodynamic therapy under a confocal microscopic system. Electrical conduction block by photodynamic reaction would be utilized as new catheter ablation for tachyarrhythmia to reduce complications. Photodynamic therapy with a short drug-light interval, which induces the oxidation mainly on the cell membrane is profitable to induce immediate myocardial cell necrosis. The necessary time to induce necrosis is important in the catheter ablation since the real-time electrocardiogram diagnosis is used to judge the treatment effect in clinical. The photosensitizer distribution changes from moment to moment during the therapy in vivo. It is necessary to investigate the time dependence of myocardial cell necrosis with various photosensitizer contact time in vitro. We measured the intracellular Ca2+ using fluo-4 AM during and after the photosensitization reaction. Talaporfin sodium was used as the photosensitizer, a CW red diode laser of 664 nm in wavelength was used for the photosensitizer excitation. Irradiance was 120 mW/cm2. The necrosis occurrence time was analyzed as the sufficient intracellular Ca2+ decrease after the membrane rupture. The photosensitizer contact time was varied up to 60 min. The necessary time for the myocardial cell necrosis decreased with photosensitizer contact time increasing and the necessary time for the myocardial cell necrosis reached a minimum value of 150 s when photosensitizer contact time was 15 min. After 15 min of photosensitizer contact time, the necessary time for the myocardial cell necrosis increased as photosensitizer contact time increased.

Original languageEnglish
Title of host publicationOptics in Health Care and Biomedical Optics VIII
EditorsYing Gu, Qingming Luo, Xingde Li, Yuguo Tang, Dan Zhu
PublisherSPIE
Volume10820
ISBN (Electronic)9781510622388
DOIs
Publication statusPublished - 2018 Jan 1
Externally publishedYes
EventOptics in Health Care and Biomedical Optics VIII 2018 - Beijing, China
Duration: 2018 Oct 112018 Oct 13

Other

OtherOptics in Health Care and Biomedical Optics VIII 2018
CountryChina
CityBeijing
Period18/10/1118/10/13

Keywords

  • drug contact time
  • Myocardial cell damage
  • NPe6
  • oxidation damage
  • photosensitization reaction
  • photosensitizer
  • talaporfin sodium

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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