Time evolution of microenvironment around cells regulated by the secretion activity and culture density of the cells

Mai Yamagishi, Yutaka Hori, Sotaro Uemura, Osamu Ohara, Yoshitaka Shirasaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cells are the minimum unit of function for a living body. Cells are in a dynamic equilibrium state with mutually interacting with the microenvironment surrounding the cells. We focused on whether cellular responses also could be regulated in the interaction of cells with the other cells and/or the microenvironment of them. Here, we try to uncover the mechanism for the macrophages to acquire a stable response at the high cell density through simulations of the way for cell-cell communication via IFN-β. As a result, the extracellular concentration of IFN-β increased rapidly, and uniformly reached the effective concentration in any place in high-density culture condition. On the other hand, the local concentration of IFN-β in the low-density culture condition could rise transiently but easily decreased by diffusion, indicating that it was hard to reach the effective concentration in the most area. The cell density-dependent differences in IFN-β field formation were also shown to have a decisive effect on the stability of cellular responses from the population of cells. Collectively, we successfully demonstrated that the cells themselves dynamically form the surrounding microenvironment to regulate their own activity.

Original languageEnglish
Title of host publicationMHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1-5
Number of pages5
ISBN (Electronic)9781538633144
DOIs
Publication statusPublished - 2018 Feb 28
Event28th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2017 - Nagoya, Japan
Duration: 2017 Dec 32017 Dec 6

Publication series

NameMHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
Volume2018-January

Other

Other28th International Symposium on Micro-NanoMechatronics and Human Science, MHS 2017
CountryJapan
CityNagoya
Period17/12/317/12/6

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ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Electrical and Electronic Engineering
  • Mechanical Engineering

Cite this

Yamagishi, M., Hori, Y., Uemura, S., Ohara, O., & Shirasaki, Y. (2018). Time evolution of microenvironment around cells regulated by the secretion activity and culture density of the cells. In MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science (pp. 1-5). (MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science; Vol. 2018-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MHS.2017.8305247