Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

Yohei Arai, Kouhei Yokoyama, Yuki Kawahara, Qi Feng, Ippei Ohta, Atsushi Shimoyama, Shinsuke Inuki, Koichi Fukase, Kazuya Kabayama, Yukari Fujimoto

Research output: Contribution to journalArticle

Abstract

As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The labeled ligands maintained the inflammatory IL-6 induction activity and enabled us to observe the internalization and colocalization of the TLR2 ligands using live-cell imaging. The time-lapse monitoring in the live-cell imaging of the fluorescence-labeled TLR2 ligand showed that TLR2/CD14 expression in the host cells enhanced the internalization of TLR2 ligand molecules.

Original languageEnglish
Pages (from-to)3824-3830
Number of pages7
JournalOrganic and Biomolecular Chemistry
Volume16
Issue number20
DOIs
Publication statusPublished - 2018 Jan 1

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Fluorescent Dyes
Ligands
ligands
Monitoring
probes
Fluorescence
fluorescence
cells
lipoproteins
Imaging techniques
Toll-Like Receptors
Optical Imaging
bacteria
Lipoproteins
Interleukin-6
Bacteria
induction
Cytokines
proteins
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes. / Arai, Yohei; Yokoyama, Kouhei; Kawahara, Yuki; Feng, Qi; Ohta, Ippei; Shimoyama, Atsushi; Inuki, Shinsuke; Fukase, Koichi; Kabayama, Kazuya; Fujimoto, Yukari.

In: Organic and Biomolecular Chemistry, Vol. 16, No. 20, 01.01.2018, p. 3824-3830.

Research output: Contribution to journalArticle

Arai, Y, Yokoyama, K, Kawahara, Y, Feng, Q, Ohta, I, Shimoyama, A, Inuki, S, Fukase, K, Kabayama, K & Fujimoto, Y 2018, 'Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes', Organic and Biomolecular Chemistry, vol. 16, no. 20, pp. 3824-3830. https://doi.org/10.1039/c7ob03205f
Arai, Yohei ; Yokoyama, Kouhei ; Kawahara, Yuki ; Feng, Qi ; Ohta, Ippei ; Shimoyama, Atsushi ; Inuki, Shinsuke ; Fukase, Koichi ; Kabayama, Kazuya ; Fujimoto, Yukari. / Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes. In: Organic and Biomolecular Chemistry. 2018 ; Vol. 16, No. 20. pp. 3824-3830.
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