Abstract
As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The labeled ligands maintained the inflammatory IL-6 induction activity and enabled us to observe the internalization and colocalization of the TLR2 ligands using live-cell imaging. The time-lapse monitoring in the live-cell imaging of the fluorescence-labeled TLR2 ligand showed that TLR2/CD14 expression in the host cells enhanced the internalization of TLR2 ligand molecules.
| Original language | English |
|---|---|
| Pages (from-to) | 3824-3830 |
| Number of pages | 7 |
| Journal | Organic and Biomolecular Chemistry |
| Volume | 16 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 2018 Jan 1 |
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ASJC Scopus subject areas
- Biochemistry
- Physical and Theoretical Chemistry
- Organic Chemistry
Cite this
Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes. / Arai, Yohei; Yokoyama, Kouhei; Kawahara, Yuki; Feng, Qi; Ohta, Ippei; Shimoyama, Atsushi; Inuki, Shinsuke; Fukase, Koichi; Kabayama, Kazuya; Fujimoto, Yukari.
In: Organic and Biomolecular Chemistry, Vol. 16, No. 20, 01.01.2018, p. 3824-3830.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes
AU - Arai, Yohei
AU - Yokoyama, Kouhei
AU - Kawahara, Yuki
AU - Feng, Qi
AU - Ohta, Ippei
AU - Shimoyama, Atsushi
AU - Inuki, Shinsuke
AU - Fukase, Koichi
AU - Kabayama, Kazuya
AU - Fujimoto, Yukari
PY - 2018/1/1
Y1 - 2018/1/1
N2 - As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The labeled ligands maintained the inflammatory IL-6 induction activity and enabled us to observe the internalization and colocalization of the TLR2 ligands using live-cell imaging. The time-lapse monitoring in the live-cell imaging of the fluorescence-labeled TLR2 ligand showed that TLR2/CD14 expression in the host cells enhanced the internalization of TLR2 ligand molecules.
AB - As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The labeled ligands maintained the inflammatory IL-6 induction activity and enabled us to observe the internalization and colocalization of the TLR2 ligands using live-cell imaging. The time-lapse monitoring in the live-cell imaging of the fluorescence-labeled TLR2 ligand showed that TLR2/CD14 expression in the host cells enhanced the internalization of TLR2 ligand molecules.
UR - http://www.scopus.com/inward/record.url?scp=85047553363&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047553363&partnerID=8YFLogxK
U2 - 10.1039/c7ob03205f
DO - 10.1039/c7ob03205f
M3 - Article
C2 - 29745411
AN - SCOPUS:85047553363
VL - 16
SP - 3824
EP - 3830
JO - Organic and Biomolecular Chemistry
JF - Organic and Biomolecular Chemistry
SN - 1477-0520
IS - 20
ER -