Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

Yohei Arai; Kouhei Yokoyama; Yuki Kawahara; Qi Feng; Ippei Ohta; Atsushi Shimoyama; Shinsuke Inuki; Koichi Fukase; Kazuya Kabayama; Yukari Fujimoto

doi:10.1039/c7ob03205f

Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

Yohei Arai, Kouhei Yokoyama, Yuki Kawahara, Qi Feng, Ippei Ohta, Atsushi Shimoyama, Shinsuke Inuki, Koichi Fukase, Kazuya Kabayama, Yukari Fujimoto

Department of Chemistry

Research output: Contribution to journal › Article

Abstract

As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The labeled ligands maintained the inflammatory IL-6 induction activity and enabled us to observe the internalization and colocalization of the TLR2 ligands using live-cell imaging. The time-lapse monitoring in the live-cell imaging of the fluorescence-labeled TLR2 ligand showed that TLR2/CD14 expression in the host cells enhanced the internalization of TLR2 ligand molecules.

Original language	English
Pages (from-to)	3824-3830
Number of pages	7
Journal	Organic and Biomolecular Chemistry
Volume	16
Issue number	20
DOIs	https://doi.org/10.1039/c7ob03205f
Publication status	Published - 2018 Jan 1

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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Arai, Y., Yokoyama, K., Kawahara, Y., Feng, Q., Ohta, I., Shimoyama, A., Inuki, S., Fukase, K., Kabayama, K., & Fujimoto, Y. (2018). Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes. Organic and Biomolecular Chemistry, 16(20), 3824-3830. https://doi.org/10.1039/c7ob03205f

Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes. / Arai, Yohei; Yokoyama, Kouhei; Kawahara, Yuki; Feng, Qi; Ohta, Ippei; Shimoyama, Atsushi; Inuki, Shinsuke; Fukase, Koichi; Kabayama, Kazuya; Fujimoto, Yukari.

In: Organic and Biomolecular Chemistry, Vol. 16, No. 20, 01.01.2018, p. 3824-3830.

Research output: Contribution to journal › Article

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