Time-resolved metabolomics of a novel trebouxiophycean alga using 13CO2 feeding

Takuro Ito, Masahiro Sugimoto, Yoshihiro Toya, Yoshitaka Ano, Norihide Kurano, Tomoyoshi Soga, Masaru Tomita

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Oil-rich algae are potentially promising as next-generation biofuel feedstock. However, the productivity of oil needs to be improved for industrial use. The biosynthesis of oil and its control mechanism have not been characterized in any algae, and understanding the metabolic network is vital to achieve the precise engineering of algae metabolic pathways. "Pseudochoricystis ellipsoidea" MBIC 11204, a novel microalgal strain, accumulates a large amount of lipids in nitrogen-deficient conditions. In this study, "P.ellipsoidea" was grown in flat flasks with continuous illumination and aeration with 1% CO2 at 25°C. During the exponential growth phase, CO2 was switched to 13C-labeled CO2 and samples were collected for time-course experiments. Seventy-eight pairs of unlabeled and uniformly 13C-labeled metabolites were quantified using a capillary electrophoresis- and liquid chromatography-mass spectrometry for ionic primary metabolites and lipids, respectively. The 13C-exchange indices of the metabolites were calculated from a concentration of unlabeled and uniformly-labeled metabolites. A hierarchical clustering analysis of the dynamics of the indices revealed 4 characteristic clusters, two of which represented rapidly-labeled metabolites, mainly composed of primary metabolites, while the two other clusters represented slowly-labeled metabolites, mainly composed of lipids. Moreover, the labeling order of these clusters was mainly matched to the metabolic process of Chlamydomonas reinhardtii, a model organism of green algae. In TCA cycle, anomalistically different of the labeling order was found. To the author's knowledge, this study for the first time in literature, characterize the features of global metabolism in "P.ellipsoidea.".

Original languageEnglish
Pages (from-to)408-415
Number of pages8
JournalJournal of Bioscience and Bioengineering
Volume116
Issue number3
DOIs
Publication statusPublished - 2013 Sep

Fingerprint

Metabolomics
Algae
Metabolites
Oils
Metabolic Networks and Pathways
Lipids
Chlamydomonas reinhardtii
Chlorophyta
Biofuels
Capillary Electrophoresis
Lighting
Liquid Chromatography
Cluster Analysis
Mass Spectrometry
Labeling
Nitrogen
Capillary electrophoresis
Growth
Biosynthesis
Liquid chromatography

Keywords

  • Carbon exchange
  • Carbon fixation
  • Lipids
  • Metabolome analysis
  • Oil-rich algae
  • Stable carbon isotope

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Time-resolved metabolomics of a novel trebouxiophycean alga using 13CO2 feeding. / Ito, Takuro; Sugimoto, Masahiro; Toya, Yoshihiro; Ano, Yoshitaka; Kurano, Norihide; Soga, Tomoyoshi; Tomita, Masaru.

In: Journal of Bioscience and Bioengineering, Vol. 116, No. 3, 09.2013, p. 408-415.

Research output: Contribution to journalArticle

Ito, Takuro ; Sugimoto, Masahiro ; Toya, Yoshihiro ; Ano, Yoshitaka ; Kurano, Norihide ; Soga, Tomoyoshi ; Tomita, Masaru. / Time-resolved metabolomics of a novel trebouxiophycean alga using 13CO2 feeding. In: Journal of Bioscience and Bioengineering. 2013 ; Vol. 116, No. 3. pp. 408-415.
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