Time response of electrical conduction block in novel cardiomyocyte wire by extra-cellular photosensitization reaction at various irradiances

Mariko Kurotsu, Emiyu Ogawa, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We studied time response of electrical conduction (EC) block in a novel cardiomyocyte wire by extra-cellular photosensitization reaction (EPR) at various irradiances. This EC block using the EPR has been studied to develop a non-thermal arrhythmia therapeutic methodology. Despite the EC block in acute phase is needed to judge therapeutic endpoint in clinical arrhythmia therapy, time response of the EC block by the EPR in acute phase hasn’t been studied. We measured the time to EC block occurrence by the EPR with intra-cellular Ca2+ concentration change using Fluo-4 AM fluorescence measurement by a confocal laser microscope system. The pattern cultivation cover glass with 10 mm Φ which had 60 μm width cultivation areas with 300 μm separations was used to form the cardiomyocyte wires. Rat cardiomyocyte with 10.8×105 cells was disseminated to the cover glasses installed in a 35 mmΦ dish. After 3 days from the dissemination, the EPR was operated to the cardiomyocyte wires for 10 min varying 3-120 mW/cm2 in 663 nm laser irradiances with 20 μg/ml talaporfin sodium. An irradiation area was approximately 60×340 μm2 on each wires. Cross correlation functions (CCF) in measured fluorescence images in every 10 s were calculated across the irradiation area. The time to EC block occurrence was defined as the time of the max difference between adjacent CCFs. By decreasing irradiances in 30-6 mW/cm2, the time to EC block occurrence became longer from 294 to 434 s. In 30-120 mW/cm2, the time to EC block occurrence was nearly constant in 300 s.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXVI
EditorsE. Duco Jansen
PublisherSPIE
ISBN (Electronic)9781628414110
DOIs
Publication statusPublished - 2014 Jan 1
EventOptical Interactions with Tissue and Cells XXVI - San Francisco, United States
Duration: 2015 Feb 82015 Feb 10

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9321
ISSN (Print)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XXVI
CountryUnited States
CitySan Francisco
Period15/2/815/2/10

Keywords

  • Cardiomyocyte
  • Cross correlation function
  • Electrical conduction
  • Extra-cellular photosensitization reaction
  • Gap junction
  • Intra-cellular calcium ion concentration
  • Talaporfin sodium
  • Time response

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Time response of electrical conduction block in novel cardiomyocyte wire by extra-cellular photosensitization reaction at various irradiances'. Together they form a unique fingerprint.

Cite this