Time-Series Analysis of Tumorigenesis in a Murine Skin Carcinogenesis Model

Yoshimasa Aoto, Kazuhiro Okumura, Tsuyoshi Hachiya, Sumitaka Hase, Yuichi Wakabayashi, Fuyuki Ishikawa, Yasubumi Sakakibara

Research output: Contribution to journalArticle

Abstract

Recent years have witnessed substantial progress in understanding tumor heterogeneity and the process of tumor progression; however, the entire process of the transition of tumors from a benign to metastatic state remains poorly understood. In the present study, we performed a prospective cancer genome-sequencing analysis by employing an experimental carcinogenesis mouse model of squamous cell carcinoma to systematically understand the evolutionary process of tumors. We surgically collected a part of a lesion of each tumor and followed the progression of these tumors in vivo over time. Comparative time-series analysis of the genomes of tumors with different fates, i.e., those that eventually metastasized and regressed, suggested that these tumors acquired and inherited different mutations. These findings suggest that despite the occurrence of an intra-tumor selection event for malignant alteration during the transformation from early- to late-stage papilloma, the fate determination of tumors might be determined at an even earlier stage.

Original languageEnglish
Article number12994
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

Fingerprint

Carcinogenesis
Skin
Neoplasms
Genome
Papilloma
Squamous Cell Carcinoma
Mutation

ASJC Scopus subject areas

  • General

Cite this

Aoto, Y., Okumura, K., Hachiya, T., Hase, S., Wakabayashi, Y., Ishikawa, F., & Sakakibara, Y. (2018). Time-Series Analysis of Tumorigenesis in a Murine Skin Carcinogenesis Model. Scientific Reports, 8(1), [12994]. https://doi.org/10.1038/s41598-018-31349-x

Time-Series Analysis of Tumorigenesis in a Murine Skin Carcinogenesis Model. / Aoto, Yoshimasa; Okumura, Kazuhiro; Hachiya, Tsuyoshi; Hase, Sumitaka; Wakabayashi, Yuichi; Ishikawa, Fuyuki; Sakakibara, Yasubumi.

In: Scientific Reports, Vol. 8, No. 1, 12994, 01.12.2018.

Research output: Contribution to journalArticle

Aoto, Y, Okumura, K, Hachiya, T, Hase, S, Wakabayashi, Y, Ishikawa, F & Sakakibara, Y 2018, 'Time-Series Analysis of Tumorigenesis in a Murine Skin Carcinogenesis Model', Scientific Reports, vol. 8, no. 1, 12994. https://doi.org/10.1038/s41598-018-31349-x
Aoto Y, Okumura K, Hachiya T, Hase S, Wakabayashi Y, Ishikawa F et al. Time-Series Analysis of Tumorigenesis in a Murine Skin Carcinogenesis Model. Scientific Reports. 2018 Dec 1;8(1). 12994. https://doi.org/10.1038/s41598-018-31349-x
Aoto, Yoshimasa ; Okumura, Kazuhiro ; Hachiya, Tsuyoshi ; Hase, Sumitaka ; Wakabayashi, Yuichi ; Ishikawa, Fuyuki ; Sakakibara, Yasubumi. / Time-Series Analysis of Tumorigenesis in a Murine Skin Carcinogenesis Model. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
@article{7a973b06dcbf42e393614b3e97cc12c8,
title = "Time-Series Analysis of Tumorigenesis in a Murine Skin Carcinogenesis Model",
abstract = "Recent years have witnessed substantial progress in understanding tumor heterogeneity and the process of tumor progression; however, the entire process of the transition of tumors from a benign to metastatic state remains poorly understood. In the present study, we performed a prospective cancer genome-sequencing analysis by employing an experimental carcinogenesis mouse model of squamous cell carcinoma to systematically understand the evolutionary process of tumors. We surgically collected a part of a lesion of each tumor and followed the progression of these tumors in vivo over time. Comparative time-series analysis of the genomes of tumors with different fates, i.e., those that eventually metastasized and regressed, suggested that these tumors acquired and inherited different mutations. These findings suggest that despite the occurrence of an intra-tumor selection event for malignant alteration during the transformation from early- to late-stage papilloma, the fate determination of tumors might be determined at an even earlier stage.",
author = "Yoshimasa Aoto and Kazuhiro Okumura and Tsuyoshi Hachiya and Sumitaka Hase and Yuichi Wakabayashi and Fuyuki Ishikawa and Yasubumi Sakakibara",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-018-31349-x",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Time-Series Analysis of Tumorigenesis in a Murine Skin Carcinogenesis Model

AU - Aoto, Yoshimasa

AU - Okumura, Kazuhiro

AU - Hachiya, Tsuyoshi

AU - Hase, Sumitaka

AU - Wakabayashi, Yuichi

AU - Ishikawa, Fuyuki

AU - Sakakibara, Yasubumi

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Recent years have witnessed substantial progress in understanding tumor heterogeneity and the process of tumor progression; however, the entire process of the transition of tumors from a benign to metastatic state remains poorly understood. In the present study, we performed a prospective cancer genome-sequencing analysis by employing an experimental carcinogenesis mouse model of squamous cell carcinoma to systematically understand the evolutionary process of tumors. We surgically collected a part of a lesion of each tumor and followed the progression of these tumors in vivo over time. Comparative time-series analysis of the genomes of tumors with different fates, i.e., those that eventually metastasized and regressed, suggested that these tumors acquired and inherited different mutations. These findings suggest that despite the occurrence of an intra-tumor selection event for malignant alteration during the transformation from early- to late-stage papilloma, the fate determination of tumors might be determined at an even earlier stage.

AB - Recent years have witnessed substantial progress in understanding tumor heterogeneity and the process of tumor progression; however, the entire process of the transition of tumors from a benign to metastatic state remains poorly understood. In the present study, we performed a prospective cancer genome-sequencing analysis by employing an experimental carcinogenesis mouse model of squamous cell carcinoma to systematically understand the evolutionary process of tumors. We surgically collected a part of a lesion of each tumor and followed the progression of these tumors in vivo over time. Comparative time-series analysis of the genomes of tumors with different fates, i.e., those that eventually metastasized and regressed, suggested that these tumors acquired and inherited different mutations. These findings suggest that despite the occurrence of an intra-tumor selection event for malignant alteration during the transformation from early- to late-stage papilloma, the fate determination of tumors might be determined at an even earlier stage.

UR - http://www.scopus.com/inward/record.url?scp=85052714554&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052714554&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-31349-x

DO - 10.1038/s41598-018-31349-x

M3 - Article

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 12994

ER -