Tissue culture methods to study neurological disorders: Establishment of immortalized Schwann cells from murine disease models

Kazuhiko Watabe, Tsuyoshi Sakamoto, Yoko Kawazoe, Makoto Michikawa, Katsuichi Miyamoto, Takashi Yamamura, Hideyuki Saya, Norie Araki

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Previously, the authors have established spontaneously immortalized cell lines from long-term cultures of normal adult mouse Schwann cells. Establishment of such Schwann cell lines derived from murine disease models may greatly facilitate studies of the cellular mechanisms of their peripheral nervous system lesions in the relevant diseases. Recently, the authors have established immortalized Schwann cell lines derived from Niemann-Pick disease type C mice (NPC; spm/spm) and globoid cell leukodystrophy mice (twitcher). In the present study, long-term cultures were maintained of Schwann cells derived from dorsal root ganglia and consecutive peripheral nerves of another NPC mouse (npcnih/npcnih, npcnih/+), myelin P0 protein-deficient mice (P0-/-, P0+/-) with their wild-type littermates (P0+/+), and neurofibromatosis type 1 gene (NF1)-deficient mice (Nf1Fcr/+) for 8-10 months, and immortalized cell lines from all these animals established spontaneously. These cell lines had spindle-shaped Schwann cell morphology and distinct Schwann cell phenotypes and retained genomic and biochemical abnormalities, sufficiently representing the in vivo pathological features of the mutant mice. These immortalized Schwann cell lines can be useful in studies of nervous system lesions in these mutant mice and relevant human disorders.

Original languageEnglish
Pages (from-to)68-78
Number of pages11
JournalNeuropathology
Volume23
Issue number1
DOIs
Publication statusPublished - 2003 Mar 1

    Fingerprint

Keywords

  • Cell line
  • Myelin P0 protein
  • Neurofibromatosis type 1
  • Niemann-Pick disease type C
  • Schwann cell

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology

Cite this