Tissue Myeloid Progenitors Differentiate into Pericytes through TGF-β Signaling in Developing Skin Vasculature

Tomoko Yamazaki, Ani Nalbandian, Yutaka Uchida, Wenling Li, Thomas D. Arnold, Yoshiaki Kubota, Seiji Yamamoto, Masatsugu Ema, Yoh suke Mukouyama

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Mural cells (pericytes and vascular smooth muscle cells) are essential for the regulation of vascular networks and maintenance of vascular integrity, but their origins are diverse in different tissues and not known in the organs that arise from the ectoderm, such as skin. Here, we show that tissue-localized myeloid progenitors contribute to pericyte development in embryonic skin vasculature. A series of in vivo fate-mapping experiments indicates that tissue myeloid progenitors differentiate into pericytes. Furthermore, depletion of tissue myeloid cells and their progenitors in PU.1 (also known as Spi1) mutants results in defective pericyte development. Fluorescence-activated cell sorting (FACS)-isolated myeloid cells and their progenitors from embryonic skin differentiate into pericytes in culture. At the molecular level, transforming growth factor-β (TGF-β) induces pericyte differentiation in culture. Furthermore, type 2 TGF-β receptor (Tgfbr2) mutants exhibit deficient pericyte development in skin vasculature. Combined, these data suggest that pericytes differentiate from tissue myeloid progenitors in the skin vasculature through TGF-β signaling.

Original languageEnglish
Pages (from-to)2991-3004
Number of pages14
JournalCell Reports
Volume18
Issue number12
DOIs
Publication statusPublished - 2017 Mar 21

Fingerprint

Pericytes
Transforming Growth Factors
Skin
Tissue
Myeloid Progenitor Cells
Blood Vessels
Cells
Ectoderm
Growth Factor Receptors
Vascular Smooth Muscle
Sorting
Smooth Muscle Myocytes
Embryonic Development
Muscle
Flow Cytometry
Fluorescence
Maintenance

Keywords

  • brain
  • capillary blood vessel
  • fate mapping
  • mural cell
  • myeloid
  • pericyte
  • skin
  • TGF-β
  • tissue macrophage
  • vascular development

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tissue Myeloid Progenitors Differentiate into Pericytes through TGF-β Signaling in Developing Skin Vasculature. / Yamazaki, Tomoko; Nalbandian, Ani; Uchida, Yutaka; Li, Wenling; Arnold, Thomas D.; Kubota, Yoshiaki; Yamamoto, Seiji; Ema, Masatsugu; Mukouyama, Yoh suke.

In: Cell Reports, Vol. 18, No. 12, 21.03.2017, p. 2991-3004.

Research output: Contribution to journalArticle

Yamazaki, T, Nalbandian, A, Uchida, Y, Li, W, Arnold, TD, Kubota, Y, Yamamoto, S, Ema, M & Mukouyama, YS 2017, 'Tissue Myeloid Progenitors Differentiate into Pericytes through TGF-β Signaling in Developing Skin Vasculature', Cell Reports, vol. 18, no. 12, pp. 2991-3004. https://doi.org/10.1016/j.celrep.2017.02.069
Yamazaki, Tomoko ; Nalbandian, Ani ; Uchida, Yutaka ; Li, Wenling ; Arnold, Thomas D. ; Kubota, Yoshiaki ; Yamamoto, Seiji ; Ema, Masatsugu ; Mukouyama, Yoh suke. / Tissue Myeloid Progenitors Differentiate into Pericytes through TGF-β Signaling in Developing Skin Vasculature. In: Cell Reports. 2017 ; Vol. 18, No. 12. pp. 2991-3004.
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