Tissue-Specific Distribution of DNA-Binding Nuclear Proteins

Hiro omi Tamura, Shunji Natori, Masuo Obinata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The DNA-binding capacity of nuclear proteins of mouse cells was examined by the protein-blotting method. Under conditions in which the lac repressor specifically binds to the lac operator, the DNA-binding nuclear proteins from different tissues showed a tissue-specific distribution, suggesting that the species and amounts of nuclear proteins with DNA binding activity differ in different tissues. When cloned eukaryotic genes were used for binding, eukaryotic DNA showed stronger binding than prokaryotic DNA. Competition experiments suggested that many nuclear proteins have different DNA binding properties from that of the prokaryotic repressor.

Original languageEnglish
Pages (from-to)87-90
Number of pages4
JournalDifferentiation
Volume23
Issue number1-3
DOIs
Publication statusPublished - 1982 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

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