Tissue-specific expression of a FMR1/β-galactosidase fusion gene in transgenic mice

Martin Hergersberg, Koichi Matsuo, Max Gassmann, Walter Schaffner, Bernhard Lüscher, Thomas Rülicke, Adriano Aguzzi

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Fragile X syndrome is one of the most common genetic causes of mental retardation, yet the mechanisms controlling expression of the fragile X mental retardation gene FMR1 are poorly understood. To identify sequences regulating FMR1 transcription, transgenic mouse lines were established using a fusion gene consisting of an E.coli β-galactosidase reporter gene (lacZ) linked to a 2.8 kb fragment spanning the 5'-region of FMR1. Five transgenic mouse lines showed lacZ expression in brain, in particular in neurons of the hippocampus and the granular layer of the cerebellum. Expression of the reporter gene was also detected in Leydig cells and spermatogonia in the testis, in many epithelia of adult mice, and in the two other steroidogenic cell types, adrenal cortex cells and ovarian follicle cells. Embryonic tissues which showed strong activity of the reporter gene included the telencephalon, the genital ridge, and the notochord. This expression pattern closely resembles the endogenous one, indicating that the 5' FMR1 gene promoter region used in this study contains most cis-acting elements regulating FMR1 transcription. / 1995 Oxford University Press.

Original languageEnglish
Pages (from-to)359-366
Number of pages8
JournalHuman Molecular Genetics
Volume4
Issue number3
DOIs
Publication statusPublished - 1995 Mar
Externally publishedYes

Fingerprint

Galactosidases
Transgenic Mice
Gene Fusion
Reporter Genes
Fusion
Fusion reactions
Genes
Tissue
Gene
Intellectual Disability
Notochord
Fragile X Syndrome
Telencephalon
Spermatogonia
Ovarian Follicle
Leydig Cells
Adrenal Cortex
Cell
Transcription
Genetic Promoter Regions

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Molecular Biology
  • Genetics(clinical)
  • Genetics

Cite this

Hergersberg, M., Matsuo, K., Gassmann, M., Schaffner, W., Lüscher, B., Rülicke, T., & Aguzzi, A. (1995). Tissue-specific expression of a FMR1/β-galactosidase fusion gene in transgenic mice. Human Molecular Genetics, 4(3), 359-366. https://doi.org/10.1093/hmg/4.3.359

Tissue-specific expression of a FMR1/β-galactosidase fusion gene in transgenic mice. / Hergersberg, Martin; Matsuo, Koichi; Gassmann, Max; Schaffner, Walter; Lüscher, Bernhard; Rülicke, Thomas; Aguzzi, Adriano.

In: Human Molecular Genetics, Vol. 4, No. 3, 03.1995, p. 359-366.

Research output: Contribution to journalArticle

Hergersberg, M, Matsuo, K, Gassmann, M, Schaffner, W, Lüscher, B, Rülicke, T & Aguzzi, A 1995, 'Tissue-specific expression of a FMR1/β-galactosidase fusion gene in transgenic mice', Human Molecular Genetics, vol. 4, no. 3, pp. 359-366. https://doi.org/10.1093/hmg/4.3.359
Hergersberg, Martin ; Matsuo, Koichi ; Gassmann, Max ; Schaffner, Walter ; Lüscher, Bernhard ; Rülicke, Thomas ; Aguzzi, Adriano. / Tissue-specific expression of a FMR1/β-galactosidase fusion gene in transgenic mice. In: Human Molecular Genetics. 1995 ; Vol. 4, No. 3. pp. 359-366.
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