Tissue-specific expression of mouse tyrosinase gene in cultured chicken cells

Toyoko Akiyama, Beth Whitaker, Mark Federspiel, Stephen H. Hughes, Hiroaki Yamamoto, Takuji Takeuchi, John Brumbaugh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A mouse tyrosinase cDNA has been combined with different promoters and inserted into several replication-competent avian leukosis proviruses and the viruses were transferred into cultured albino chick cells by viral infection. Expression of the tyrosinase gene depended on one of four promoter sequences: the resident constitutive promoter (Rous sarcoma virus long-terminal repeat; RSV-LTR), 471 bp from the mouse tyrosinase gene-associated promoter, 519 bp from the Japanese quail tyrosinase gene associated promoter, or 369 bp from the quail tyrosinase promoter. The infected cells expressed tyrosinase and produced pigment which could be seen with the light microscope. Immunofluorescence microscopy, using an anti mouse tyrosinase T1-specific antibody, also showed the presence of mouse tyrosinase. When infected with the same viral titer, gene expression was highest with the constitutive LTR promoter. The quail tyrosinase promoter, while less efficient than the LTR, was more efficient than the other tyrosinase promoter. Fibroblasts and hepatocytes infected with the construct carrying the constitutive promoter or the truncated quail promoter expressed tyrosinase. The mouse and quail promoters appeared to show tissue-specific expression since fibroblasts and hepatocytes infected with viruses carrying these promoters did not express mouse tyrosinase. Toxicity is associated with constitutive expression of tyrosinase in nonmelanocytes. Therefore the viruses that carry the tissue specific promoters should be useful for in vivo studies.

Original languageEnglish
Pages (from-to)154-162
Number of pages9
JournalExperimental Cell Research
Volume214
Issue number1
DOIs
Publication statusPublished - 1994
Externally publishedYes

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Monophenol Monooxygenase
Chickens
Cultured Cells
Genes
Quail
Hepatocytes
Fibroblasts
Avian Leukosis Virus
Viruses
Gene Expression
Rous sarcoma virus
Coturnix
Proviruses
Viral Genes
Terminal Repeat Sequences
Virus Diseases
Fluorescence Microscopy
Complementary DNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Akiyama, T., Whitaker, B., Federspiel, M., Hughes, S. H., Yamamoto, H., Takeuchi, T., & Brumbaugh, J. (1994). Tissue-specific expression of mouse tyrosinase gene in cultured chicken cells. Experimental Cell Research, 214(1), 154-162. https://doi.org/10.1006/excr.1994.1244

Tissue-specific expression of mouse tyrosinase gene in cultured chicken cells. / Akiyama, Toyoko; Whitaker, Beth; Federspiel, Mark; Hughes, Stephen H.; Yamamoto, Hiroaki; Takeuchi, Takuji; Brumbaugh, John.

In: Experimental Cell Research, Vol. 214, No. 1, 1994, p. 154-162.

Research output: Contribution to journalArticle

Akiyama, T, Whitaker, B, Federspiel, M, Hughes, SH, Yamamoto, H, Takeuchi, T & Brumbaugh, J 1994, 'Tissue-specific expression of mouse tyrosinase gene in cultured chicken cells', Experimental Cell Research, vol. 214, no. 1, pp. 154-162. https://doi.org/10.1006/excr.1994.1244
Akiyama T, Whitaker B, Federspiel M, Hughes SH, Yamamoto H, Takeuchi T et al. Tissue-specific expression of mouse tyrosinase gene in cultured chicken cells. Experimental Cell Research. 1994;214(1):154-162. https://doi.org/10.1006/excr.1994.1244
Akiyama, Toyoko ; Whitaker, Beth ; Federspiel, Mark ; Hughes, Stephen H. ; Yamamoto, Hiroaki ; Takeuchi, Takuji ; Brumbaugh, John. / Tissue-specific expression of mouse tyrosinase gene in cultured chicken cells. In: Experimental Cell Research. 1994 ; Vol. 214, No. 1. pp. 154-162.
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