TY - JOUR
T1 - Titanium Culture Vessel Capable of Controlling Culture Temperature for Evaluation of Cell Thermotolerance
AU - Imashiro, Chikahiro
AU - Ida, Yuta
AU - Miyata, Shogo
AU - Komotori, Jun
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grant number 18J12482, 20J00337, 20K20172, by the Research Grant of Keio Leading-edge Laboratory of Science and Technology, and by the Light Metal Educational Foundation, Inc. Chikahiro Imashiro is a JSPS research fellow. The authors would like to thank Ms. Haruka Takeshita, a graduate student at Keio University for her help in the discussion.
Publisher Copyright:
© 2022 Japan Institute of Metals (JIM). All rights reserved.
PY - 2022
Y1 - 2022
N2 - Surgery, radiation therapy, and chemical therapy have been reported as the main treatments for cancer, which is one of the deadliest reported diseases. However, because of the high invasiveness of patients, cancer hyperthermia has been studied as a non-invasive treatment. Hyperthermia uses a difference in thermal tolerance between normal and cancer cells and provides an affected part thermal stimulation to kill cancer cells selectively. To develop effective conditions for hyperthermia, an in vitro study to evaluate the thermal tolerance is required. However, because the existing cell culture vessels cannot control the culture temperature, genuine thermal tolerance of cells cannot be investigated appropriately. To reveal the critical temperature proper for hyperthermia, we developed a culture device controlling culture temperature. With the developed device, culture temperature was regulated considerably more quickly than the conventional method with the existing vessels, which enabled us to study the thermal tolerance of cells appropriately. For the control of culture temperature, the device has a titanium culture substrate, where a Peltier element is adhered. Because the biocompatibility of the device was confirmed, the difference in thermal tolerance between normal and cancer cells was investigated using the developed device as well as normal human dermal fibroblasts and Michigan Cancer Foundation-7 as model cell species. Therefore, it was confirmed that cancer cells were more sensitive to thermal stimulation than normal cells, which was qualitatively consistent with previous studies. Thus, our developed device can be used to investigate the thermal tolerance of each cell species, which will contribute to the development of cancer hyperthermia.
AB - Surgery, radiation therapy, and chemical therapy have been reported as the main treatments for cancer, which is one of the deadliest reported diseases. However, because of the high invasiveness of patients, cancer hyperthermia has been studied as a non-invasive treatment. Hyperthermia uses a difference in thermal tolerance between normal and cancer cells and provides an affected part thermal stimulation to kill cancer cells selectively. To develop effective conditions for hyperthermia, an in vitro study to evaluate the thermal tolerance is required. However, because the existing cell culture vessels cannot control the culture temperature, genuine thermal tolerance of cells cannot be investigated appropriately. To reveal the critical temperature proper for hyperthermia, we developed a culture device controlling culture temperature. With the developed device, culture temperature was regulated considerably more quickly than the conventional method with the existing vessels, which enabled us to study the thermal tolerance of cells appropriately. For the control of culture temperature, the device has a titanium culture substrate, where a Peltier element is adhered. Because the biocompatibility of the device was confirmed, the difference in thermal tolerance between normal and cancer cells was investigated using the developed device as well as normal human dermal fibroblasts and Michigan Cancer Foundation-7 as model cell species. Therefore, it was confirmed that cancer cells were more sensitive to thermal stimulation than normal cells, which was qualitatively consistent with previous studies. Thus, our developed device can be used to investigate the thermal tolerance of each cell species, which will contribute to the development of cancer hyperthermia.
KW - Cancer cells
KW - Cell adherence
KW - Cell proliferation
KW - Cultured cell
KW - Fine particle peening
KW - Heat shock protein
KW - Hyperthermia treatment
KW - Metallic cell culture device
KW - Normal cells
KW - Surface modification
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U2 - 10.2320/matertrans.MT-Z2021018
DO - 10.2320/matertrans.MT-Z2021018
M3 - Article
AN - SCOPUS:85125579297
VL - 63
SP - 373
EP - 378
JO - Materials Transactions
JF - Materials Transactions
SN - 1345-9678
IS - 3
ER -