TOF-SIMS imaging of halide/thiocyanate anions and hydrogen sulfide in mouse kidney sections using silver-deposited plates

Noriyuki Akahoshi, Itsuko Ishizaki, Masayuki Naya, Toshihiko Maekawa, Shougo Yamazoe, Tadashi Horiuchi, Mayumi Kajimura, Yoshiharu Ohashi, Makoto Suematsu, Isao Ishii

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In vivo imaging of reactive small molecule metabolites with high spatial resolution and specificity could give clues to understanding pathophysiology of various diseases. We herein applied time of flight-secondary ion mass spectrometry (TOF-SIMS) to newly developed silverdeposited plates that were stamped on mouse tissues, and succeeded in visualization of halide (Cl-, Br-, and I-) and pseudohalide thiocyanate (SCN-) anions, a class of substrates for neutrophils/eosinophil peroxidases to produce hypohalous acids (HOX/OX- mixture; X: (pseudo) halides) , as well as hydrogen sulfide (H2S) . Forty-micrometer frozen mouse kidney sections on cover glasses were attached to 37 °C preheated silver-deposited plates and incubated at -10 °C for 1 h. After sputter cleaning to remove surface contaminants, the plates were analyzed by TOF-SIMS to identify distribution of Br-, AgBr 2-, I-, AgI 2 -, SCN-, as well as S 2- and AgS- as products of tissue-derived H 2S. Br-, AgBr 2 -, I-, and SCN- anions were mainly distributed in core regions including the inner medulla and inner stripe of the outer medulla (except for I-) , rather than outer regions such as the cortex and outer stripe of the outer medulla. AgI 2 - anion was spread over the whole kidney, although its levels were relatively low. In contrast, S 2- and AgS- anions were mainly present in the outer regions. To our knowledge, this is the first imaging study to reveal the distribution of (pseudo) halides and H 2S in animal tissue sections.

Original languageEnglish
Pages (from-to)1859-1864
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume402
Issue number5
DOIs
Publication statusPublished - 2012 Feb

Fingerprint

Secondary Ion Mass Spectrometry
Hydrogen Sulfide
Secondary ion mass spectrometry
Silver
Anions
Kidney
Imaging techniques
Tissue
Eosinophil Peroxidase
Metabolites
Glass
Cleaning
Animals
Neutrophils
Visualization
Impurities
Molecules
Acids
thiocyanate
Substrates

Keywords

  • Halide
  • Hydrogen sulfide
  • Imaging
  • Sliver-deposited plate
  • Thiocyanate
  • TOF-SIMS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

TOF-SIMS imaging of halide/thiocyanate anions and hydrogen sulfide in mouse kidney sections using silver-deposited plates. / Akahoshi, Noriyuki; Ishizaki, Itsuko; Naya, Masayuki; Maekawa, Toshihiko; Yamazoe, Shougo; Horiuchi, Tadashi; Kajimura, Mayumi; Ohashi, Yoshiharu; Suematsu, Makoto; Ishii, Isao.

In: Analytical and Bioanalytical Chemistry, Vol. 402, No. 5, 02.2012, p. 1859-1864.

Research output: Contribution to journalArticle

Akahoshi, N, Ishizaki, I, Naya, M, Maekawa, T, Yamazoe, S, Horiuchi, T, Kajimura, M, Ohashi, Y, Suematsu, M & Ishii, I 2012, 'TOF-SIMS imaging of halide/thiocyanate anions and hydrogen sulfide in mouse kidney sections using silver-deposited plates', Analytical and Bioanalytical Chemistry, vol. 402, no. 5, pp. 1859-1864. https://doi.org/10.1007/s00216-011-5647-2
Akahoshi, Noriyuki ; Ishizaki, Itsuko ; Naya, Masayuki ; Maekawa, Toshihiko ; Yamazoe, Shougo ; Horiuchi, Tadashi ; Kajimura, Mayumi ; Ohashi, Yoshiharu ; Suematsu, Makoto ; Ishii, Isao. / TOF-SIMS imaging of halide/thiocyanate anions and hydrogen sulfide in mouse kidney sections using silver-deposited plates. In: Analytical and Bioanalytical Chemistry. 2012 ; Vol. 402, No. 5. pp. 1859-1864.
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AU - Akahoshi, Noriyuki

AU - Ishizaki, Itsuko

AU - Naya, Masayuki

AU - Maekawa, Toshihiko

AU - Yamazoe, Shougo

AU - Horiuchi, Tadashi

AU - Kajimura, Mayumi

AU - Ohashi, Yoshiharu

AU - Suematsu, Makoto

AU - Ishii, Isao

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N2 - In vivo imaging of reactive small molecule metabolites with high spatial resolution and specificity could give clues to understanding pathophysiology of various diseases. We herein applied time of flight-secondary ion mass spectrometry (TOF-SIMS) to newly developed silverdeposited plates that were stamped on mouse tissues, and succeeded in visualization of halide (Cl-, Br-, and I-) and pseudohalide thiocyanate (SCN-) anions, a class of substrates for neutrophils/eosinophil peroxidases to produce hypohalous acids (HOX/OX- mixture; X: (pseudo) halides) , as well as hydrogen sulfide (H2S) . Forty-micrometer frozen mouse kidney sections on cover glasses were attached to 37 °C preheated silver-deposited plates and incubated at -10 °C for 1 h. After sputter cleaning to remove surface contaminants, the plates were analyzed by TOF-SIMS to identify distribution of Br-, AgBr 2-, I-, AgI 2 -, SCN-, as well as S 2- and AgS- as products of tissue-derived H 2S. Br-, AgBr 2 -, I-, and SCN- anions were mainly distributed in core regions including the inner medulla and inner stripe of the outer medulla (except for I-) , rather than outer regions such as the cortex and outer stripe of the outer medulla. AgI 2 - anion was spread over the whole kidney, although its levels were relatively low. In contrast, S 2- and AgS- anions were mainly present in the outer regions. To our knowledge, this is the first imaging study to reveal the distribution of (pseudo) halides and H 2S in animal tissue sections.

AB - In vivo imaging of reactive small molecule metabolites with high spatial resolution and specificity could give clues to understanding pathophysiology of various diseases. We herein applied time of flight-secondary ion mass spectrometry (TOF-SIMS) to newly developed silverdeposited plates that were stamped on mouse tissues, and succeeded in visualization of halide (Cl-, Br-, and I-) and pseudohalide thiocyanate (SCN-) anions, a class of substrates for neutrophils/eosinophil peroxidases to produce hypohalous acids (HOX/OX- mixture; X: (pseudo) halides) , as well as hydrogen sulfide (H2S) . Forty-micrometer frozen mouse kidney sections on cover glasses were attached to 37 °C preheated silver-deposited plates and incubated at -10 °C for 1 h. After sputter cleaning to remove surface contaminants, the plates were analyzed by TOF-SIMS to identify distribution of Br-, AgBr 2-, I-, AgI 2 -, SCN-, as well as S 2- and AgS- as products of tissue-derived H 2S. Br-, AgBr 2 -, I-, and SCN- anions were mainly distributed in core regions including the inner medulla and inner stripe of the outer medulla (except for I-) , rather than outer regions such as the cortex and outer stripe of the outer medulla. AgI 2 - anion was spread over the whole kidney, although its levels were relatively low. In contrast, S 2- and AgS- anions were mainly present in the outer regions. To our knowledge, this is the first imaging study to reveal the distribution of (pseudo) halides and H 2S in animal tissue sections.

KW - Halide

KW - Hydrogen sulfide

KW - Imaging

KW - Sliver-deposited plate

KW - Thiocyanate

KW - TOF-SIMS

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