Toll-like receptors, NOD1, and NOD2 in oral epithelial cells

Y. Sugawara, A. Uehara, Yukari Fujimoto, S. Kusumoto, K. Fukase, K. Shibata, S. Sugawara, T. Sasano, H. Takada

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Oral epithelium might be the first barrier against oral bacteria in periodontal tissue. We hypothesized that oral epithelium is endowed with innate immune receptors for bacterial components, which play roles in host defense against bacterial infection without being accompanied by excessive inflammatory responses. We found clear expression of Toll-like receptor (TLR)4 as well as TLR2, and strong expression of NOD1 and NOD2 in normal oral epithelial tissues by immunohistochemical analysis. We also showed that primary oral epithelial cells in culture expressed these molecules using PCR, flow cytometry, and immunostaining. In inflamed oral epithelium, cell-surface localizations of TLR2 and TLR4 were more clearly observed than in healthy tissue. Upon stimulation with synthetic ligands for these receptors, the expression of β-defensin 2 was markedly up-regulated. These findings indicate that these molecules in oral epithelial cells are functional receptors that induce antibacterial responses.

Original languageEnglish
Pages (from-to)524-529
Number of pages6
JournalJournal of Dental Research
Volume85
Issue number6
DOIs
Publication statusPublished - 2006 Jun
Externally publishedYes

Fingerprint

Toll-Like Receptors
Epithelium
Epithelial Cells
Artificial Receptors
Defensins
Toll-Like Receptor 4
Bacterial Infections
Flow Cytometry
Cell Culture Techniques
Ligands
Bacteria
Polymerase Chain Reaction

Keywords

  • β-defensin 2
  • Epithelium
  • Inflammation
  • NOD
  • TLR

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Sugawara, Y., Uehara, A., Fujimoto, Y., Kusumoto, S., Fukase, K., Shibata, K., ... Takada, H. (2006). Toll-like receptors, NOD1, and NOD2 in oral epithelial cells. Journal of Dental Research, 85(6), 524-529. https://doi.org/10.1177/154405910608500609

Toll-like receptors, NOD1, and NOD2 in oral epithelial cells. / Sugawara, Y.; Uehara, A.; Fujimoto, Yukari; Kusumoto, S.; Fukase, K.; Shibata, K.; Sugawara, S.; Sasano, T.; Takada, H.

In: Journal of Dental Research, Vol. 85, No. 6, 06.2006, p. 524-529.

Research output: Contribution to journalArticle

Sugawara, Y, Uehara, A, Fujimoto, Y, Kusumoto, S, Fukase, K, Shibata, K, Sugawara, S, Sasano, T & Takada, H 2006, 'Toll-like receptors, NOD1, and NOD2 in oral epithelial cells', Journal of Dental Research, vol. 85, no. 6, pp. 524-529. https://doi.org/10.1177/154405910608500609
Sugawara, Y. ; Uehara, A. ; Fujimoto, Yukari ; Kusumoto, S. ; Fukase, K. ; Shibata, K. ; Sugawara, S. ; Sasano, T. ; Takada, H. / Toll-like receptors, NOD1, and NOD2 in oral epithelial cells. In: Journal of Dental Research. 2006 ; Vol. 85, No. 6. pp. 524-529.
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