Top3β is an RNA topoisomerase that works with fragile X syndrome protein to promote synapse formation

Dongyi Xu, Weiping Shen, Rong Guo, Yutong Xue, Wei Peng, Jian Sima, Jay Yang, Alexei Sharov, Subramanya Srikantan, Jiandong Yang, David Fox, Yong Qian, Jennifer L. Martindale, Yulan Piao, James Machamer, Samit R. Joshi, Subhasis Mohanty, Albert C. Shaw, Thomas E. Lloyd, Grant W. BrownMinoru Ko, Myriam Gorospe, Sige Zou, Weidong Wang

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Topoisomerases are crucial for solving DNA topological problems, but they have not been linked to RNA metabolism. Here we show that human topoisomerase 3β (Top3β) is an RNA topoisomerase that biochemically and genetically interacts with FMRP, a protein that is deficient in fragile X syndrome and is known to regulate the translation of mRNAs that are important for neuronal function, abnormalities of which are linked to autism. Notably, the FMRP-Top3β interaction is abolished by a disease-associated mutation of FMRP, suggesting that Top3β may contribute to the pathogenesis of mental disorders. Top3β binds multiple mRNAs encoded by genes with neuronal functions linked to schizophrenia and autism. Expression of one such gene, that encoding protein tyrosine kinase 2 (ptk2, also known as focal adhesion kinase or FAK), is reduced in the neuromuscular junctions of Top3β mutant flies. Synapse formation is defective in Top3β mutant flies and mice, as well as in FMRP mutant flies and mice. Our findings suggest that Top3β acts as an RNA topoisomerase and works with FMRP to promote the expression of mRNAs that are crucial for neurodevelopment and mental health.

Original languageEnglish
Pages (from-to)1238-1247
Number of pages10
JournalNature Neuroscience
Volume16
Issue number9
DOIs
Publication statusPublished - 2013 Sep
Externally publishedYes

Fingerprint

Fragile X Syndrome
Diptera
Synapses
RNA
Autistic Disorder
Focal Adhesion Kinase 1
Fragile X Mental Retardation Protein
Focal Adhesion Protein-Tyrosine Kinases
Messenger RNA
Proteins
Neuromuscular Junction
Protein Biosynthesis
Mental Disorders
Schizophrenia
Mental Health
Mutation
DNA
Genes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Top3β is an RNA topoisomerase that works with fragile X syndrome protein to promote synapse formation. / Xu, Dongyi; Shen, Weiping; Guo, Rong; Xue, Yutong; Peng, Wei; Sima, Jian; Yang, Jay; Sharov, Alexei; Srikantan, Subramanya; Yang, Jiandong; Fox, David; Qian, Yong; Martindale, Jennifer L.; Piao, Yulan; Machamer, James; Joshi, Samit R.; Mohanty, Subhasis; Shaw, Albert C.; Lloyd, Thomas E.; Brown, Grant W.; Ko, Minoru; Gorospe, Myriam; Zou, Sige; Wang, Weidong.

In: Nature Neuroscience, Vol. 16, No. 9, 09.2013, p. 1238-1247.

Research output: Contribution to journalArticle

Xu, D, Shen, W, Guo, R, Xue, Y, Peng, W, Sima, J, Yang, J, Sharov, A, Srikantan, S, Yang, J, Fox, D, Qian, Y, Martindale, JL, Piao, Y, Machamer, J, Joshi, SR, Mohanty, S, Shaw, AC, Lloyd, TE, Brown, GW, Ko, M, Gorospe, M, Zou, S & Wang, W 2013, 'Top3β is an RNA topoisomerase that works with fragile X syndrome protein to promote synapse formation', Nature Neuroscience, vol. 16, no. 9, pp. 1238-1247. https://doi.org/10.1038/nn.3479
Xu, Dongyi ; Shen, Weiping ; Guo, Rong ; Xue, Yutong ; Peng, Wei ; Sima, Jian ; Yang, Jay ; Sharov, Alexei ; Srikantan, Subramanya ; Yang, Jiandong ; Fox, David ; Qian, Yong ; Martindale, Jennifer L. ; Piao, Yulan ; Machamer, James ; Joshi, Samit R. ; Mohanty, Subhasis ; Shaw, Albert C. ; Lloyd, Thomas E. ; Brown, Grant W. ; Ko, Minoru ; Gorospe, Myriam ; Zou, Sige ; Wang, Weidong. / Top3β is an RNA topoisomerase that works with fragile X syndrome protein to promote synapse formation. In: Nature Neuroscience. 2013 ; Vol. 16, No. 9. pp. 1238-1247.
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