Topical application of a novel, water-soluble γ-tocopherol derivative prevents UV-induced skin damage in mice

Shingo Yasuoka, Jiro Takata, Yoshiharu Karube, Eiko Katoh, Toshi Tsuzuki, Junko Kizu, Masao Tsuchiya, Shizuko Kobayashi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We investigated whether the topical application of a novel, water-soluble γ-tocopherol (γ-Toc) derivative, γ-tocopherol-N,N- dimethylglycinate hydrochloride (γ-TDMG), could protect against UV-induced skin damage in hairless mice. Topical pre-or post-application of a 5% (93 mM) γ-TDMG solution in water/ propylene glycol/ethanol (2:1:2) significantly prevented sunburn cell formation, lipid peroxidation and edema/inflammation that were induced by exposure to a single dose of UV irradiation of 5 kJ/m 2 (290-380 nm, maximum 312 nm). This effect was greater than that seen with two α-Toc derivatives, α-tocopherol acetate (α-TA) and α-tocopherol-N,N-dimethylglycinate (α-TDMG). When a 5% solution of γ-TDMG was applied to mouse skin for 1 h, cutaneous γ-Toc increased by 25-fold after 24 h; levels of cutaneous α-Toc increased by only two-and eight-fold in α-TDMG and α-TA treated skins, respectively. These findings indicated that γ-TDMG immediately converted to γ-Toc in the skin and suggest that ability of γ-TDMG to protect the skin from the damaging effects of irradiation was due to its conversion to γ-Toc. When a 5% solution of γ-Toc was applied to mouse skin for 1 h, cutaneous γ-Toc rapidly increased by 25-fold, but fell to baseline levels by 24 h. In contrast, the concentration of γ-Toc in skin that was treated with γ-TDMG similarly increased, but these high levels were maintained after 24 h. These results suggest that γ-TDMG may be a more effective source of γ-Toc in skin. Thus, the topical application of γ-TDMG may be efficacious for the prevention of UV-B-induced skin damage.

Original languageEnglish
Pages (from-to)908-913
Number of pages6
JournalPhotochemistry and Photobiology
Volume81
Issue number4
DOIs
Publication statusPublished - 2005 Jul

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tocopherol
Tocopherols
mice
Skin
damage
Derivatives
Water
water
edema
irradiation
hydrochlorides
propylene
lipids
glycols
acetates
ethyl alcohol
Irradiation
dosage
Propylene Glycol
cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Topical application of a novel, water-soluble γ-tocopherol derivative prevents UV-induced skin damage in mice. / Yasuoka, Shingo; Takata, Jiro; Karube, Yoshiharu; Katoh, Eiko; Tsuzuki, Toshi; Kizu, Junko; Tsuchiya, Masao; Kobayashi, Shizuko.

In: Photochemistry and Photobiology, Vol. 81, No. 4, 07.2005, p. 908-913.

Research output: Contribution to journalArticle

Yasuoka, S, Takata, J, Karube, Y, Katoh, E, Tsuzuki, T, Kizu, J, Tsuchiya, M & Kobayashi, S 2005, 'Topical application of a novel, water-soluble γ-tocopherol derivative prevents UV-induced skin damage in mice', Photochemistry and Photobiology, vol. 81, no. 4, pp. 908-913. https://doi.org/10.1562/2004-09-02-RA-300R2.1
Yasuoka, Shingo ; Takata, Jiro ; Karube, Yoshiharu ; Katoh, Eiko ; Tsuzuki, Toshi ; Kizu, Junko ; Tsuchiya, Masao ; Kobayashi, Shizuko. / Topical application of a novel, water-soluble γ-tocopherol derivative prevents UV-induced skin damage in mice. In: Photochemistry and Photobiology. 2005 ; Vol. 81, No. 4. pp. 908-913.
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abstract = "We investigated whether the topical application of a novel, water-soluble γ-tocopherol (γ-Toc) derivative, γ-tocopherol-N,N- dimethylglycinate hydrochloride (γ-TDMG), could protect against UV-induced skin damage in hairless mice. Topical pre-or post-application of a 5{\%} (93 mM) γ-TDMG solution in water/ propylene glycol/ethanol (2:1:2) significantly prevented sunburn cell formation, lipid peroxidation and edema/inflammation that were induced by exposure to a single dose of UV irradiation of 5 kJ/m 2 (290-380 nm, maximum 312 nm). This effect was greater than that seen with two α-Toc derivatives, α-tocopherol acetate (α-TA) and α-tocopherol-N,N-dimethylglycinate (α-TDMG). When a 5{\%} solution of γ-TDMG was applied to mouse skin for 1 h, cutaneous γ-Toc increased by 25-fold after 24 h; levels of cutaneous α-Toc increased by only two-and eight-fold in α-TDMG and α-TA treated skins, respectively. These findings indicated that γ-TDMG immediately converted to γ-Toc in the skin and suggest that ability of γ-TDMG to protect the skin from the damaging effects of irradiation was due to its conversion to γ-Toc. When a 5{\%} solution of γ-Toc was applied to mouse skin for 1 h, cutaneous γ-Toc rapidly increased by 25-fold, but fell to baseline levels by 24 h. In contrast, the concentration of γ-Toc in skin that was treated with γ-TDMG similarly increased, but these high levels were maintained after 24 h. These results suggest that γ-TDMG may be a more effective source of γ-Toc in skin. Thus, the topical application of γ-TDMG may be efficacious for the prevention of UV-B-induced skin damage.",
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