Topical application of a novel, water-soluble γ-tocopherol derivative prevents UV-induced skin damage in mice

Shingo Yasuoka, Jiro Takata, Yoshiharu Karube, Eiko Katoh, Toshi Tsuzuki, Junko Kizu, Masao Tsuchiya, Shizuko Kobayashi

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Abstract

We investigated whether the topical application of a novel, water-soluble γ-tocopherol (γ-Toc) derivative, γ-tocopherol-N,N- dimethylglycinate hydrochloride (γ-TDMG), could protect against UV-induced skin damage in hairless mice. Topical pre-or post-application of a 5% (93 mM) γ-TDMG solution in water/ propylene glycol/ethanol (2:1:2) significantly prevented sunburn cell formation, lipid peroxidation and edema/inflammation that were induced by exposure to a single dose of UV irradiation of 5 kJ/m 2 (290-380 nm, maximum 312 nm). This effect was greater than that seen with two α-Toc derivatives, α-tocopherol acetate (α-TA) and α-tocopherol-N,N-dimethylglycinate (α-TDMG). When a 5% solution of γ-TDMG was applied to mouse skin for 1 h, cutaneous γ-Toc increased by 25-fold after 24 h; levels of cutaneous α-Toc increased by only two-and eight-fold in α-TDMG and α-TA treated skins, respectively. These findings indicated that γ-TDMG immediately converted to γ-Toc in the skin and suggest that ability of γ-TDMG to protect the skin from the damaging effects of irradiation was due to its conversion to γ-Toc. When a 5% solution of γ-Toc was applied to mouse skin for 1 h, cutaneous γ-Toc rapidly increased by 25-fold, but fell to baseline levels by 24 h. In contrast, the concentration of γ-Toc in skin that was treated with γ-TDMG similarly increased, but these high levels were maintained after 24 h. These results suggest that γ-TDMG may be a more effective source of γ-Toc in skin. Thus, the topical application of γ-TDMG may be efficacious for the prevention of UV-B-induced skin damage.

Original languageEnglish
Pages (from-to)908-913
Number of pages6
JournalPhotochemistry and Photobiology
Volume81
Issue number4
DOIs
Publication statusPublished - 2005 Jul 1

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ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

Yasuoka, S., Takata, J., Karube, Y., Katoh, E., Tsuzuki, T., Kizu, J., Tsuchiya, M., & Kobayashi, S. (2005). Topical application of a novel, water-soluble γ-tocopherol derivative prevents UV-induced skin damage in mice. Photochemistry and Photobiology, 81(4), 908-913. https://doi.org/10.1562/2004-09-02-RA-300R2.1