Toxin in bullous impetigo and staphylococcal scalded-skin syndrome targets desmoglein 1

Masayuki Amagai, N. Matsuyoshi, Z. H. Wang, C. Andl, J. R. Stanley

Research output: Contribution to journalArticle

347 Citations (Scopus)

Abstract

Exfoliative toxin A, produced by Staphylococcus aureus, causes blisters in bullous impetigo and its more generalized form, staphylococcal scalded-skin syndrome. The toxin shows exquisite specificity in causing loss of cell adhesion only in the superficial epidermis. Although exfoliative toxin A has the structure of a serine protease, a target protein has not been identified. Desmoglein (Dsg) 1, a desmosomal cadherin that mediates cell-cell adhesion, may be the target of exfoliative toxin A, because it is the target of autoantibodies in pemphigus foliacues, in which blisters form with identical tissue specificity and histology. We show here that exfoliative toxin A cleaved mouse and human Dsg1, but not closely related cadherins such as Dsg3. We demonstrate this specific cleavage in cell culture, in neonatal mouse skin and with recombinant Dsg1, and conclude that Dsg1 is the specific receptor for exfoliative toxin A cleavage. This unique proteolytic attack on the desmosome cause a blister just below the stratum corneum, which forms the epidermal barrier, presumably allowing the bacteria in bullous impetigo to proliferate and spread beneath this barrier.

Original languageEnglish
Pages (from-to)1275-1277
Number of pages3
JournalNature Medicine
Volume6
Issue number11
DOIs
Publication statusPublished - 2000

Fingerprint

Staphylococcal Scalded Skin Syndrome
Exfoliatins
Desmoglein 1
Impetigo
Skin
Blister
Cell adhesion
Cell Adhesion
Desmosomal Cadherins
Desmosomes
Organ Specificity
Histology
Pemphigus
Serine Proteases
Cadherins
Cell culture
Epidermis
Autoantibodies
Cornea
Staphylococcus aureus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Toxin in bullous impetigo and staphylococcal scalded-skin syndrome targets desmoglein 1. / Amagai, Masayuki; Matsuyoshi, N.; Wang, Z. H.; Andl, C.; Stanley, J. R.

In: Nature Medicine, Vol. 6, No. 11, 2000, p. 1275-1277.

Research output: Contribution to journalArticle

Amagai, Masayuki ; Matsuyoshi, N. ; Wang, Z. H. ; Andl, C. ; Stanley, J. R. / Toxin in bullous impetigo and staphylococcal scalded-skin syndrome targets desmoglein 1. In: Nature Medicine. 2000 ; Vol. 6, No. 11. pp. 1275-1277.
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