Toxoplasma gondii

Secretion of a Potent Nucleoside Triphosphate Hydrolase into the Parasitophorous Vacuole

L. D. Sibley, I. R. Niesman, T. Asai, T. Takeuchi

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Toxoplasma gondii is an obligate intracellular parasite capable of invading a wide range of host cells where it resides in a specially modified compartment termed the parasitophorous vacuole. This compartment provides a protected environment for the parasite which enters a rapid growth phase shortly after invasion. To identify functional components of this interface, we have used immunolabeling and cell fractionation to localize the potent nucleoside triphosphate hydrolase (NTPase) produced by Toxoplasma. In extracellular tachyzoites, NTPase was not exposed on the cell surface but was localized in small vesicles, called dense granules, scattered throughout the cell cytoplasm. Shortly following invasion, NTPase was secreted from dense granules and occupied the lumen of the parasitophorous vacuole where it was often associated with the intravacuolar network. NTPase was exclusively found in the supernatant in cell fractionation studies of both extracellular tachyzoites lysed by freeze-thaw and following secretion into the parasitophorous vacuole. These studies provide evidence for the rapid secretion of NTPase into the parasitophorous vacuole, where it may play a key role in processing of nucleotides for purine salvage by the parasite.

Original languageEnglish
Pages (from-to)301-311
Number of pages11
JournalExperimental Parasitology
Volume79
Issue number3
DOIs
Publication statusPublished - 1994 Nov

Fingerprint

N-Glycosyl Hydrolases
Toxoplasma
Vacuoles
Cell Fractionation
Parasites
Purine Nucleotides
Cytoplasm
triphosphoric acid
Growth

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology
  • Immunology

Cite this

Toxoplasma gondii : Secretion of a Potent Nucleoside Triphosphate Hydrolase into the Parasitophorous Vacuole. / Sibley, L. D.; Niesman, I. R.; Asai, T.; Takeuchi, T.

In: Experimental Parasitology, Vol. 79, No. 3, 11.1994, p. 301-311.

Research output: Contribution to journalArticle

Sibley, L. D. ; Niesman, I. R. ; Asai, T. ; Takeuchi, T. / Toxoplasma gondii : Secretion of a Potent Nucleoside Triphosphate Hydrolase into the Parasitophorous Vacuole. In: Experimental Parasitology. 1994 ; Vol. 79, No. 3. pp. 301-311.
@article{3f18f09c312a4df5bb580841a66ff1aa,
title = "Toxoplasma gondii: Secretion of a Potent Nucleoside Triphosphate Hydrolase into the Parasitophorous Vacuole",
abstract = "Toxoplasma gondii is an obligate intracellular parasite capable of invading a wide range of host cells where it resides in a specially modified compartment termed the parasitophorous vacuole. This compartment provides a protected environment for the parasite which enters a rapid growth phase shortly after invasion. To identify functional components of this interface, we have used immunolabeling and cell fractionation to localize the potent nucleoside triphosphate hydrolase (NTPase) produced by Toxoplasma. In extracellular tachyzoites, NTPase was not exposed on the cell surface but was localized in small vesicles, called dense granules, scattered throughout the cell cytoplasm. Shortly following invasion, NTPase was secreted from dense granules and occupied the lumen of the parasitophorous vacuole where it was often associated with the intravacuolar network. NTPase was exclusively found in the supernatant in cell fractionation studies of both extracellular tachyzoites lysed by freeze-thaw and following secretion into the parasitophorous vacuole. These studies provide evidence for the rapid secretion of NTPase into the parasitophorous vacuole, where it may play a key role in processing of nucleotides for purine salvage by the parasite.",
author = "Sibley, {L. D.} and Niesman, {I. R.} and T. Asai and T. Takeuchi",
year = "1994",
month = "11",
doi = "10.1006/expr.1994.1093",
language = "English",
volume = "79",
pages = "301--311",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Toxoplasma gondii

T2 - Secretion of a Potent Nucleoside Triphosphate Hydrolase into the Parasitophorous Vacuole

AU - Sibley, L. D.

AU - Niesman, I. R.

AU - Asai, T.

AU - Takeuchi, T.

PY - 1994/11

Y1 - 1994/11

N2 - Toxoplasma gondii is an obligate intracellular parasite capable of invading a wide range of host cells where it resides in a specially modified compartment termed the parasitophorous vacuole. This compartment provides a protected environment for the parasite which enters a rapid growth phase shortly after invasion. To identify functional components of this interface, we have used immunolabeling and cell fractionation to localize the potent nucleoside triphosphate hydrolase (NTPase) produced by Toxoplasma. In extracellular tachyzoites, NTPase was not exposed on the cell surface but was localized in small vesicles, called dense granules, scattered throughout the cell cytoplasm. Shortly following invasion, NTPase was secreted from dense granules and occupied the lumen of the parasitophorous vacuole where it was often associated with the intravacuolar network. NTPase was exclusively found in the supernatant in cell fractionation studies of both extracellular tachyzoites lysed by freeze-thaw and following secretion into the parasitophorous vacuole. These studies provide evidence for the rapid secretion of NTPase into the parasitophorous vacuole, where it may play a key role in processing of nucleotides for purine salvage by the parasite.

AB - Toxoplasma gondii is an obligate intracellular parasite capable of invading a wide range of host cells where it resides in a specially modified compartment termed the parasitophorous vacuole. This compartment provides a protected environment for the parasite which enters a rapid growth phase shortly after invasion. To identify functional components of this interface, we have used immunolabeling and cell fractionation to localize the potent nucleoside triphosphate hydrolase (NTPase) produced by Toxoplasma. In extracellular tachyzoites, NTPase was not exposed on the cell surface but was localized in small vesicles, called dense granules, scattered throughout the cell cytoplasm. Shortly following invasion, NTPase was secreted from dense granules and occupied the lumen of the parasitophorous vacuole where it was often associated with the intravacuolar network. NTPase was exclusively found in the supernatant in cell fractionation studies of both extracellular tachyzoites lysed by freeze-thaw and following secretion into the parasitophorous vacuole. These studies provide evidence for the rapid secretion of NTPase into the parasitophorous vacuole, where it may play a key role in processing of nucleotides for purine salvage by the parasite.

UR - http://www.scopus.com/inward/record.url?scp=0027973621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027973621&partnerID=8YFLogxK

U2 - 10.1006/expr.1994.1093

DO - 10.1006/expr.1994.1093

M3 - Article

VL - 79

SP - 301

EP - 311

JO - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

IS - 3

ER -