TRAF6 and C-SRC induce synergistic AP-1 activation via PI3-kinase-AKT-JNK pathway

Megumi Funakoshi-Tago, Kenji Tago, Yoshiko Sonoda, Shin Ichi Tominaga, Tadashi Kasahara

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Interleukin-1 (IL-1) induces multiple genes via activation of transcription factors that include NF-κB and activator protein-1 (AP-1). We found that IL-1-mediated c-Src activation was required for AP-1 activation, but not for NF-κB activation and also revealed that c-Src-induced AP-1 activation was enhanced synergistically by the coexpression of TNF receptor associated factor 6 (TRAF6). In addition, c-Src interacts with TRAF6 in response to IL-1 and this interaction is required for c-Src activity. However, neither dominant negative mutants of TRAF6 (TRAF6 DN) nor kinase-dead mutant of c-Src (c-Src KD) counteracted each-induced AP-1 activation, suggesting no hierarchy between these two molecules. During the TRAF6 and c-Src-induced AP-1 activation, phosphatidylinositol 3 (PI3)-kinase, its downstream signaling molecule, Akt and c-Jun N-terminal kinase (JNK) were significantly activated and inhibition of these kinase activities down-regulated AP-1 activation through the suppression of c-fos expression. Furthermore, TRAF6 and c-Src-induced JNK activation was significantly inhibited by PI3-kinase inhibitor or a dominant negative mutant of Akt (Akt DN). Taken together, our results demonstrate that c-Src and TRAF6 are key mediators of IL-1-induced AP-1 activation and provide evidence of cross talk between c-Src and TRAF6 molecules through PI3 kinase-Akt-JNK pathways.

Original languageEnglish
Pages (from-to)1257-1268
Number of pages12
JournalEuropean Journal of Biochemistry
Volume270
Issue number6
DOIs
Publication statusPublished - 2003 Mar 1

Keywords

  • Activator protein-1 (AP-1)
  • NF-κB activation
  • Src kinase
  • TRAF6
  • c-Jun N-terminal kinase (JNK)

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'TRAF6 and C-SRC induce synergistic AP-1 activation via PI3-kinase-AKT-JNK pathway'. Together they form a unique fingerprint.

  • Cite this