Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Mark G. Carter, Alexei A. Sharov, Vincent VanBuren, Dawood B. Dudekula, Condie E. Carmack, Charlie Nelson, Minoru S.H. Ko

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)

Abstract

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

Original languageEnglish
Pages (from-to)R61
JournalGenome biology
Volume6
Issue number7
Publication statusPublished - 2005
Externally publishedYes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

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