Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Mark G. Carter; Alexei A. Sharov; Vincent VanBuren; Dawood B. Dudekula; Condie E. Carmack; Charlie Nelson; Minoru S.H. Ko

Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Mark G. Carter, Alexei A. Sharov, Vincent VanBuren, Dawood B. Dudekula, Condie E. Carmack, Charlie Nelson, Minoru S.H. Ko

Research output: Contribution to journal › Article › peer-review

96 Citations (Scopus)

Abstract

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

Original language	English
Pages (from-to)	R61
Journal	Genome biology
Volume	6
Issue number	7
Publication status	Published - 2005
Externally published	Yes

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics
Cell Biology

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abstract = "The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.",

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AU - Nelson, Charlie

AU - Ko, Minoru S.H.

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N2 - The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

AB - The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

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Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Abstract

ASJC Scopus subject areas

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