Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Mark G. Carter; Alexei A. Sharov; Vincent VanBuren; Dawood B. Dudekula; Condie E. Carmack; Charlie Nelson; Minoru S.H. Ko

Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Mark G. Carter, Alexei A. Sharov, Vincent VanBuren, Dawood B. Dudekula, Condie E. Carmack, Charlie Nelson, Minoru S.H. Ko

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

Original language	English
Pages (from-to)	R61
Journal	Genome biology
Volume	6
Issue number	7
Publication status	Published - 2005
Externally published	Yes

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics
Cell Biology

Cite this

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title = "Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.",

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author = "Carter, {Mark G.} and Sharov, {Alexei A.} and Vincent VanBuren and Dudekula, {Dawood B.} and Carmack, {Condie E.} and Charlie Nelson and Ko, {Minoru S.H.}",

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AU - Carmack, Condie E.

AU - Nelson, Charlie

AU - Ko, Minoru S.H.

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AB - The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

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Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Abstract

ASJC Scopus subject areas

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