Abstract
The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.
| Original language | English |
|---|---|
| Pages (from-to) | R61 |
| Journal | Genome biology |
| Volume | 6 |
| Issue number | 7 |
| Publication status | Published - 2005 |
| Externally published | Yes |
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Cell Biology
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Cite this
Carter, M. G., Sharov, A. A., VanBuren, V., Dudekula, D. B., Carmack, C. E., Nelson, C., & Ko, M. S. H. (2005). Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray. Genome biology, 6(7), R61.