Transcription factor protein expression patterns by neural or neuronal progenitor cells of adult monkey subventricular zone

A. B. Tonchev, T. Yamashima, K. Sawamoto, Hideyuki Okano

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The anterior subventricular zone of the adult mammalian brain contains progenitor cells which are upregulated after cerebral ischemia. We have previously reported that while a part of the progenitors residing in adult monkey anterior subventricular zone travels to the olfactory bulb, many of these cells sustain location in the anterior subventricular zone for months after injury, exhibiting a phenotype of either neural or neuronal precursors. Here we show that ischemia increased the numbers of anterior subventricular zone progenitor cells expressing developmentally regulated transcription factors including Pax6 (paired-box 6), Emx2 (empty spiracles-homeobox 2), Sox 1-3 (sex determining region Y-box 1-3), Ngn1 (neurogenin 1), Dlx1,5 (distalless-homeobox 1,5), Olig1,3 (oligodendrocyte lineage gene 1,3) and Nkx2.2 (Nk-box 2.2), as compared with control brains. Analysis of transcription factor protein expression by sustained neural or neuronal precursors in anterior subventricular zone revealed that these two cell types were positive for characteristic sets of transcription factors. The proteins Pax6, Emx2, Sox2,3 and Olig1 were predominantly localized to dividing neural precursors while the factors Sox1, Ngn1, Dlx1,5, Olig2 and Nkx2.2 were mainly expressed by neuronal precursors. Further, differences between monkeys and non-primate mammals emerged, related to expression patterns of Pax6, Olig2 and Dlx2. Our results suggest that a complex network of developmental signals might be involved in the specification of primate progenitor cells.

Original languageEnglish
Pages (from-to)1355-1367
Number of pages13
JournalNeuroscience
Volume139
Issue number4
DOIs
Publication statusPublished - 2006

Fingerprint

Lateral Ventricles
Haplorhini
Transcription Factors
Stem Cells
Homeobox Genes
Proteins
Olfactory Bulb
Oligodendroglia
Brain
Brain Ischemia
Primates
Mammals
Ischemia
Phenotype
Wounds and Injuries
Genes

Keywords

  • adult neurogenesis
  • cell fate
  • cerebral ischemia
  • developmental signal
  • primate

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Transcription factor protein expression patterns by neural or neuronal progenitor cells of adult monkey subventricular zone. / Tonchev, A. B.; Yamashima, T.; Sawamoto, K.; Okano, Hideyuki.

In: Neuroscience, Vol. 139, No. 4, 2006, p. 1355-1367.

Research output: Contribution to journalArticle

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