Transcriptional activation of ATF6 by endoplasmic reticulum stressors

Takushi Namba, Tomoaki Ishihara, Ken ichiro Tanaka, Tatsuya Hoshino, Tohru Mizushima

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Previous studies have shown that modification of activating transcription factor 6 (ATF6) protein is important for the endoplasmic reticulum (ER) stress response; ER stressors stimulate the degradation of ATF6 by Site-1 protease (S1P) and Site-2 protease (S2P) into p50-ATF6, which acts as a transcription factor. In the current study, we found that all of the ER stressors tested (such as thapsigargin) up-regulate ATF6 mRNA expression. As thapsigargin did not affect the stability of the ATF6 mRNA, it was concluded that this up-regulation is due to transcriptional activation of ATF6. An inhibitor of S1P suppressed this up-regulation of ATF6 mRNA expression and putative ATF6-binding elements in the promoter of ATF6 were identified, suggesting that p50-ATF6 positively regulates the gene expression of ATF6. Since cells over-expressing ATF6 showed an enhanced ER stress response, we propose that up-regulation of ATF6 mRNA expression is involved in enhancing the ER stress response.

Original languageEnglish
Pages (from-to)543-548
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume355
Issue number2
DOIs
Publication statusPublished - 2007 Apr 6
Externally publishedYes

Fingerprint

Activating Transcription Factor 6
Endoplasmic Reticulum
Transcriptional Activation
Chemical activation
Endoplasmic Reticulum Stress
Up-Regulation
Messenger RNA
Thapsigargin

Keywords

  • ATF6
  • Endoplasmic reticulum
  • ER stress response
  • S1P
  • Transcriptional activation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Transcriptional activation of ATF6 by endoplasmic reticulum stressors. / Namba, Takushi; Ishihara, Tomoaki; Tanaka, Ken ichiro; Hoshino, Tatsuya; Mizushima, Tohru.

In: Biochemical and Biophysical Research Communications, Vol. 355, No. 2, 06.04.2007, p. 543-548.

Research output: Contribution to journalArticle

Namba, Takushi ; Ishihara, Tomoaki ; Tanaka, Ken ichiro ; Hoshino, Tatsuya ; Mizushima, Tohru. / Transcriptional activation of ATF6 by endoplasmic reticulum stressors. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 355, No. 2. pp. 543-548.
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