Transcriptional pattern of a novel gene, expressed specifically after the point-of-no-return during sexualization, in planaria

Sumitaka Hase, Kazuya Kobayashi, Ryo Koyanagi, Motonori Hoshi, Midori Matsumoto

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have investigated sexualization of asexual worms in the planarian Dugesia ryukyuensis. During sexualization there is a point from which an animal cannot return to the asexual state (point-of-no-return). To isolate the genes related to the point-of-no-return, we performed differential screening and isolated one novel gene that was expressed specifically in yolk glands of the worms after the point-of-no-return and named it Dryg. It encoded 655 amino acids with a predicted molecular mass of 79 kDa. We performed a series of experiments using Dryg as a molecular marker in the yolk gland. At first, we monitored how the yolk gland was formed during sexualization. The expression in sexualizing worms at stage 3 is limited to a single type of cell that has characteristics of neoblasts, the totipotent somatic cells; however, the expression is observed in the yolk gland in sexualized worms. Furthermore, we monitored yolk glands for expression during regeneration. The original yolk glands seem to disappear after ablation, then new yolk glands appeared along the ventral nerve cords. Because this expression pattern looks like that of sexualizing worms at stage 3, we speculate that yolk gland cells may differentiate from neoblasts during regeneration as observed during sexualization.

Original languageEnglish
Pages (from-to)585-592
Number of pages8
JournalDevelopment Genes and Evolution
Volume212
Issue number12
Publication statusPublished - 2003 Jan 1

Fingerprint

Planaria
Planarians
regeneration
Dugesia
ventral nerve cord
gene
Regeneration
somatic cells
ablation
Genes
genes
amino acid
cells
molecular weight
screening
amino acids
genetic markers
animal
Amino Acids
animals

Keywords

  • Neoblast
  • Planaria
  • Regeneration
  • Sexualization
  • Yolk gland

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Transcriptional pattern of a novel gene, expressed specifically after the point-of-no-return during sexualization, in planaria. / Hase, Sumitaka; Kobayashi, Kazuya; Koyanagi, Ryo; Hoshi, Motonori; Matsumoto, Midori.

In: Development Genes and Evolution, Vol. 212, No. 12, 01.01.2003, p. 585-592.

Research output: Contribution to journalArticle

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