Transcriptional regulation of choline acetyltransferase gene by cyclic AMP

Hidemi Misawa, Ryosuke Takahashi, Takeo Deguchi

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The effect of cyclic AMP on the gene expression of cholinc acetyltransferase (ChAT) was studied in NG108-15, mouse neuroblastoma and rat glioma hybrid cell lines. Addition of dibutyryl cyclic AMP to the culture medium increased both the ChAT mRNA level and ChAT activity twofold. Polymerase chain reaction analysis of the ChAT mRNA indicated that, among the multiple mRNA species, M-type mRNA was transcribed most efficiently, with or without the addition of dibutyryl cyclic AMP. The 5́ region of the mouse ChAT gene was ligated to the bacterial chloramphenicol acetyltransferase gene, and the expression of chloramphenicol acetyltransferase activity was determined by transaction analysis. Cyclic AMP derivatives enhanced the reporter gene expression in both transiently and stably transfected cells. DNA deletion analysis indicated that the intron region downstream of the M-type exon is necessary for the cyclic AMP responsiveness, and that cyclic AMP derivatives increase ChAT gene transcription mainly from M-type promoter. These results suggest that a cis-acting DNA element that confers the cyclic AMP responsiveness of the ChAT gene is present in the intron downstream of the M-type exon.

Original languageEnglish
Pages (from-to)1383-1387
Number of pages5
JournalJournal of Neurochemistry
Volume60
Issue number4
Publication statusPublished - 1993 Apr
Externally publishedYes

Fingerprint

Acetyltransferases
Choline O-Acetyltransferase
Cyclic AMP
Genes
Bucladesine
Messenger RNA
Chloramphenicol O-Acetyltransferase
Gene Expression
Gene expression
Introns
Exons
Derivatives
Hybrid Cells
Polymerase chain reaction
DNA
Transcription
Neuroblastoma
Reporter Genes
Glioma
Culture Media

Keywords

  • Choline acetyltransferase
  • Cyclic AMP
  • DNA transfection
  • Gene expression

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Transcriptional regulation of choline acetyltransferase gene by cyclic AMP. / Misawa, Hidemi; Takahashi, Ryosuke; Deguchi, Takeo.

In: Journal of Neurochemistry, Vol. 60, No. 4, 04.1993, p. 1383-1387.

Research output: Contribution to journalArticle

Misawa, Hidemi ; Takahashi, Ryosuke ; Deguchi, Takeo. / Transcriptional regulation of choline acetyltransferase gene by cyclic AMP. In: Journal of Neurochemistry. 1993 ; Vol. 60, No. 4. pp. 1383-1387.
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