Transcriptional regulation of steroid receptor coactivator-1 (SRC-1) in glucocorticoid action

Isao Kurihara, H. Shibata, T. Suzuki, T. Ando, S. Kobayashi, M. Hayashi, I. Saito, T. Saruta

Research output: Contribution to journalArticle

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Abstract

Diverse mechanisms of steroid receptor action have been clarified in recent years, as a consequence of the discovery of multiple coactivators. Among them, steroid receptor coactivator-1 (SRC-1) is a member of the p160 coactivator families, which are 160 kDa proteins that interact with steroid receptors in a hormone-sensitive manner. Since coactivators function as transcriptional power boosters, subtle changes in coactivator expression levels in certain cells markedly change of receptor-mediated transcriptional activity. Expression of the glucocorticoid receptor (GR) has been shown to be autoregulated in glucocorticoid action, i.e., GR is downregulated by its cognate ligand, indicating that this autoregulation of GR may protect target cells against excessive hormone action. In the present study, we examined whether coactivator expression levels are also regulated by glucocorticoids. Among several coactivators, the SRC-1 mRNA level was downregulated by dexamethasone treatment in rat tissues, such as liver, heart, kidney, stomach, and cerebrum, in vivo. We also demonstrated dexamethasone-mediated downregulation of SRC-1 mRNA and its protein levels in rat renal mesangial cells in vitro. These results suggest that ligand-mediated downregulation of SRC-1 is crucial in the physiology of glucocorticoid action.

Original languageEnglish
Pages (from-to)1033-1038
Number of pages6
JournalEndocrine Research
Volume26
Issue number4
Publication statusPublished - 2000

Fingerprint

Nuclear Receptor Coactivator 1
Glucocorticoids
Glucocorticoid Receptors
Down-Regulation
Steroid Receptors
Dexamethasone
Hormones
Ligands
Messenger RNA
Mesangial Cells
Cerebrum
Stomach
Proteins
Homeostasis
Kidney
Liver

ASJC Scopus subject areas

  • Endocrinology

Cite this

Kurihara, I., Shibata, H., Suzuki, T., Ando, T., Kobayashi, S., Hayashi, M., ... Saruta, T. (2000). Transcriptional regulation of steroid receptor coactivator-1 (SRC-1) in glucocorticoid action. Endocrine Research, 26(4), 1033-1038.

Transcriptional regulation of steroid receptor coactivator-1 (SRC-1) in glucocorticoid action. / Kurihara, Isao; Shibata, H.; Suzuki, T.; Ando, T.; Kobayashi, S.; Hayashi, M.; Saito, I.; Saruta, T.

In: Endocrine Research, Vol. 26, No. 4, 2000, p. 1033-1038.

Research output: Contribution to journalArticle

Kurihara, I, Shibata, H, Suzuki, T, Ando, T, Kobayashi, S, Hayashi, M, Saito, I & Saruta, T 2000, 'Transcriptional regulation of steroid receptor coactivator-1 (SRC-1) in glucocorticoid action', Endocrine Research, vol. 26, no. 4, pp. 1033-1038.
Kurihara I, Shibata H, Suzuki T, Ando T, Kobayashi S, Hayashi M et al. Transcriptional regulation of steroid receptor coactivator-1 (SRC-1) in glucocorticoid action. Endocrine Research. 2000;26(4):1033-1038.
Kurihara, Isao ; Shibata, H. ; Suzuki, T. ; Ando, T. ; Kobayashi, S. ; Hayashi, M. ; Saito, I. ; Saruta, T. / Transcriptional regulation of steroid receptor coactivator-1 (SRC-1) in glucocorticoid action. In: Endocrine Research. 2000 ; Vol. 26, No. 4. pp. 1033-1038.
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