Transcriptome analysis of the anhydrobiotic cell line Pv11 infers the mechanism of desiccation tolerance and recovery

Takahiro Yamada, Yoshitaka Suetsugu, Ruslan Deviatiiarov, Oleg Gusev, Richard Cornette, Alexander Nesmelov, Noriko Hiroi, Takahiro Kikawada, Akira Funahashi

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Abstract

The larvae of the African midge, Polypedilum vanderplanki, can enter an ametabolic state called anhydrobiosis to overcome fatal desiccation stress. The Pv11 cell line, derived from P. vanderplanki embryo, shows desiccation tolerance when treated with trehalose before desiccation and resumes proliferation after rehydration. However, the molecular mechanisms of this desiccation tolerance remain unknown. Here, we performed high-throughput CAGE-seq of mRNA and a differentially expressed gene analysis in trehalose-treated, desiccated, and rehydrated Pv11 cells, followed by gene ontology analysis of the identified differentially expressed genes. We detected differentially expressed genes after trehalose treatment involved in various stress responses, detoxification of harmful chemicals, and regulation of oxidoreduction that were upregulated. In the desiccation phase, L-isoaspartyl methyltransferase and heat shock proteins were upregulated and ribosomal proteins were downregulated. Analysis of differentially expressed genes during rehydration supported the notion that homologous recombination, nucleotide excision repair, and non-homologous recombination were involved in the recovery process. This study provides initial insights into the molecular mechanisms underlying the extreme desiccation tolerance of Pv11 cells.

Original languageEnglish
Article number17941
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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Desiccation
Gene Expression Profiling
Cell Line
Trehalose
Fluid Therapy
Genes
Gene Ontology
Ribosomal Proteins
Homologous Recombination
Methyltransferases
Heat-Shock Proteins
DNA Repair
Genetic Recombination
Larva
Down-Regulation
Embryonic Structures
Messenger RNA

ASJC Scopus subject areas

  • General

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Transcriptome analysis of the anhydrobiotic cell line Pv11 infers the mechanism of desiccation tolerance and recovery. / Yamada, Takahiro; Suetsugu, Yoshitaka; Deviatiiarov, Ruslan; Gusev, Oleg; Cornette, Richard; Nesmelov, Alexander; Hiroi, Noriko; Kikawada, Takahiro; Funahashi, Akira.

In: Scientific Reports, Vol. 8, No. 1, 17941, 01.12.2018.

Research output: Contribution to journalArticle

Yamada, Takahiro ; Suetsugu, Yoshitaka ; Deviatiiarov, Ruslan ; Gusev, Oleg ; Cornette, Richard ; Nesmelov, Alexander ; Hiroi, Noriko ; Kikawada, Takahiro ; Funahashi, Akira. / Transcriptome analysis of the anhydrobiotic cell line Pv11 infers the mechanism of desiccation tolerance and recovery. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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