Transformation of epithelial Madin-Darby canine kidney cells with p60(v- src) induces expression of membrane-type 1 matrix metalloproteinase and invasiveness

Yoshifumi Kadono, Yasunori Okada, Mikio Namiki, Motoharu Seiki, Hiroshi Sato

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Membrane-type 1 matrix metalloproteinase (MT1-MMP) is expressed both in carcinoma cells and in surrounding stromal fibroblasts. MT1-MMP localizes to the surface of tumor cells and is thought to play an important role in tumor invasion. To analyze the mechanism of MT1-MMP gene expression in epithelial tumor cells, the dog kidney epithelial cell line Madin-Darby canine kidney (MDCK) was transformed by oncogenes, including v-src, and expression of MT1- MMP was examined. Transformation of MDCK cells with v-src resulted in loss of cell-to-cell contacts and morphological change. Expression of MT1-MMP in v- src-transformed cells was identified by Northern and Western blotting. Gelatin zymography analysis showed that progelatinase A in the culture medium was processed from latent to activated form by MDCK cells transformed with v- src. The MDCK cells transformed by v-src were tumorigenic in the subcutis (ectopic) and kidney (orthotopic) of nude mice and spontaneously metastasized to the lung after orthotopic implantation. These results suggest that MT1- MMP induced by v-src transformation may promote invasiveness of transformed cells.

Original languageEnglish
Pages (from-to)2240-2244
Number of pages5
JournalCancer Research
Volume58
Issue number10
Publication statusPublished - 1998 May 15

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Oncogene Protein pp60(v-src)
Matrix Metalloproteinase 14
Madin Darby Canine Kidney Cells
Epithelial Cells
Kidney
src Genes
Neoplasms
Gelatin
Nude Mice
Northern Blotting
Culture Media
Canidae
Fibroblasts
Western Blotting
Dogs
Carcinoma
Gene Expression
Cell Line
Lung

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Transformation of epithelial Madin-Darby canine kidney cells with p60(v- src) induces expression of membrane-type 1 matrix metalloproteinase and invasiveness. / Kadono, Yoshifumi; Okada, Yasunori; Namiki, Mikio; Seiki, Motoharu; Sato, Hiroshi.

In: Cancer Research, Vol. 58, No. 10, 15.05.1998, p. 2240-2244.

Research output: Contribution to journalArticle

Kadono, Yoshifumi ; Okada, Yasunori ; Namiki, Mikio ; Seiki, Motoharu ; Sato, Hiroshi. / Transformation of epithelial Madin-Darby canine kidney cells with p60(v- src) induces expression of membrane-type 1 matrix metalloproteinase and invasiveness. In: Cancer Research. 1998 ; Vol. 58, No. 10. pp. 2240-2244.
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