Abstract
Membrane-type 1 matrix metalloproteinase (MT1-MMP) is expressed both in carcinoma cells and in surrounding stromal fibroblasts. MT1-MMP localizes to the surface of tumor cells and is thought to play an important role in tumor invasion. To analyze the mechanism of MT1-MMP gene expression in epithelial tumor cells, the dog kidney epithelial cell line Madin-Darby canine kidney (MDCK) was transformed by oncogenes, including v-src, and expression of MT1- MMP was examined. Transformation of MDCK cells with v-src resulted in loss of cell-to-cell contacts and morphological change. Expression of MT1-MMP in v- src-transformed cells was identified by Northern and Western blotting. Gelatin zymography analysis showed that progelatinase A in the culture medium was processed from latent to activated form by MDCK cells transformed with v- src. The MDCK cells transformed by v-src were tumorigenic in the subcutis (ectopic) and kidney (orthotopic) of nude mice and spontaneously metastasized to the lung after orthotopic implantation. These results suggest that MT1- MMP induced by v-src transformation may promote invasiveness of transformed cells.
| Original language | English |
|---|---|
| Pages (from-to) | 2240-2244 |
| Number of pages | 5 |
| Journal | Cancer Research |
| Volume | 58 |
| Issue number | 10 |
| Publication status | Published - 1998 May 15 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
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Transformation of epithelial Madin-Darby canine kidney cells with p60(v- src) induces expression of membrane-type 1 matrix metalloproteinase and invasiveness. / Kadono, Yoshifumi; Okada, Yasunori; Namiki, Mikio; Seiki, Motoharu; Sato, Hiroshi.
In: Cancer Research, Vol. 58, No. 10, 15.05.1998, p. 2240-2244.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transformation of epithelial Madin-Darby canine kidney cells with p60(v- src) induces expression of membrane-type 1 matrix metalloproteinase and invasiveness
AU - Kadono, Yoshifumi
AU - Okada, Yasunori
AU - Namiki, Mikio
AU - Seiki, Motoharu
AU - Sato, Hiroshi
PY - 1998/5/15
Y1 - 1998/5/15
N2 - Membrane-type 1 matrix metalloproteinase (MT1-MMP) is expressed both in carcinoma cells and in surrounding stromal fibroblasts. MT1-MMP localizes to the surface of tumor cells and is thought to play an important role in tumor invasion. To analyze the mechanism of MT1-MMP gene expression in epithelial tumor cells, the dog kidney epithelial cell line Madin-Darby canine kidney (MDCK) was transformed by oncogenes, including v-src, and expression of MT1- MMP was examined. Transformation of MDCK cells with v-src resulted in loss of cell-to-cell contacts and morphological change. Expression of MT1-MMP in v- src-transformed cells was identified by Northern and Western blotting. Gelatin zymography analysis showed that progelatinase A in the culture medium was processed from latent to activated form by MDCK cells transformed with v- src. The MDCK cells transformed by v-src were tumorigenic in the subcutis (ectopic) and kidney (orthotopic) of nude mice and spontaneously metastasized to the lung after orthotopic implantation. These results suggest that MT1- MMP induced by v-src transformation may promote invasiveness of transformed cells.
AB - Membrane-type 1 matrix metalloproteinase (MT1-MMP) is expressed both in carcinoma cells and in surrounding stromal fibroblasts. MT1-MMP localizes to the surface of tumor cells and is thought to play an important role in tumor invasion. To analyze the mechanism of MT1-MMP gene expression in epithelial tumor cells, the dog kidney epithelial cell line Madin-Darby canine kidney (MDCK) was transformed by oncogenes, including v-src, and expression of MT1- MMP was examined. Transformation of MDCK cells with v-src resulted in loss of cell-to-cell contacts and morphological change. Expression of MT1-MMP in v- src-transformed cells was identified by Northern and Western blotting. Gelatin zymography analysis showed that progelatinase A in the culture medium was processed from latent to activated form by MDCK cells transformed with v- src. The MDCK cells transformed by v-src were tumorigenic in the subcutis (ectopic) and kidney (orthotopic) of nude mice and spontaneously metastasized to the lung after orthotopic implantation. These results suggest that MT1- MMP induced by v-src transformation may promote invasiveness of transformed cells.
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M3 - Article
VL - 58
SP - 2240
EP - 2244
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 10
ER -