Transformation of Ipecac (Cephaelis ipecacuanha) with Agrobacterium rhizogenes

Kayo Yoshimatsu, Koichiro Shimomura, Mami Yamazaki, Kazuki Saito, Fumiyuki Kiuchi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Transformed root cultures of ipecac (Cephaelis ipecacuanha A. Richard), one of the recalcitrant woody plant species for Agrobacterium-mediated transformation, were established by co-culturing of in vitro petiole segments with Agrobacterium rhizogenes ATCC 15834. Southern blot analysis of the established roots revealed that only the TL-DNA was integrated into the plant genome without incorporation of the TR-DNA. The transformed roots grew slowly on phytohormone-free solid medium and adventitious shoots were regenerated after over 6 months of culture on HF, half-strength Murashige and Skoog (1/2 MS) medium in the dark. The individually separated transformed shoots developed into plantlets on phytohormone-free solid medium at 25°C under 16h/day light, and the plants demonstrated wider leaves, shorter internodes and vigorous root growth compared to non-transformed plants. Effects of basal media and auxins on the growth and the ipecac alkaloid production of the transformed roots were investigated either under light or in the dark. The roots cultured in the dark grew well in Gamborg B5 (B5) liquid medium containing 0.5mg/L IBA and yielded 112 mg/L of cephaeline and 14 mg/L emetine after 8 weeks of culture.

Original languageEnglish
Pages (from-to)1018-1023
Number of pages6
JournalPlanta Medica
Volume69
Issue number11
DOIs
Publication statusPublished - 2003 Nov 1
Externally publishedYes

Keywords

  • Agrobacterium rhizogenes
  • Cephaelis ipecacuanha
  • Ipecac
  • Isoquinoline alkaloids
  • Transformation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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