Transformation of Ipecac (Cephaelis ipecacuanha) with Agrobacterium rhizogenes

Kayo Yoshimatsu, Koichiro Shimomura, Mami Yamazaki, Kazuki Saito, Fumiyuki Kiuchi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Transformed root cultures of ipecac (Cephaelis ipecacuanha A. Richard), one of the recalcitrant woody plant species for Agrobacterium-mediated transformation, were established by co-culturing of in vitro petiole segments with Agrobacterium rhizogenes ATCC 15834. Southern blot analysis of the established roots revealed that only the TL-DNA was integrated into the plant genome without incorporation of the TR-DNA. The transformed roots grew slowly on phytohormone-free solid medium and adventitious shoots were regenerated after over 6 months of culture on HF, half-strength Murashige and Skoog (1/2 MS) medium in the dark. The individually separated transformed shoots developed into plantlets on phytohormone-free solid medium at 25°C under 16h/day light, and the plants demonstrated wider leaves, shorter internodes and vigorous root growth compared to non-transformed plants. Effects of basal media and auxins on the growth and the ipecac alkaloid production of the transformed roots were investigated either under light or in the dark. The roots cultured in the dark grew well in Gamborg B5 (B5) liquid medium containing 0.5mg/L IBA and yielded 112 mg/L of cephaeline and 14 mg/L emetine after 8 weeks of culture.

Original languageEnglish
Pages (from-to)1018-1023
Number of pages6
JournalPlanta Medica
Volume69
Issue number11
DOIs
Publication statusPublished - 2003 Nov
Externally publishedYes

Fingerprint

Cephaelis
Ipecac
Carapichea ipecacuanha
Agrobacterium
Plant Growth Regulators
Rhizobium rhizogenes
Emetine
Indoleacetic Acids
DNA
Alkaloids
Plant Genome
Light
Genes
plant hormones
Growth
Southern Blotting
emetine
Liquids
adventitious shoots
indole butyric acid

Keywords

  • Agrobacterium rhizogenes
  • Cephaelis ipecacuanha
  • Ipecac
  • Isoquinoline alkaloids
  • Transformation

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Transformation of Ipecac (Cephaelis ipecacuanha) with Agrobacterium rhizogenes. / Yoshimatsu, Kayo; Shimomura, Koichiro; Yamazaki, Mami; Saito, Kazuki; Kiuchi, Fumiyuki.

In: Planta Medica, Vol. 69, No. 11, 11.2003, p. 1018-1023.

Research output: Contribution to journalArticle

Yoshimatsu, K, Shimomura, K, Yamazaki, M, Saito, K & Kiuchi, F 2003, 'Transformation of Ipecac (Cephaelis ipecacuanha) with Agrobacterium rhizogenes', Planta Medica, vol. 69, no. 11, pp. 1018-1023. https://doi.org/10.1055/s-2003-45149
Yoshimatsu, Kayo ; Shimomura, Koichiro ; Yamazaki, Mami ; Saito, Kazuki ; Kiuchi, Fumiyuki. / Transformation of Ipecac (Cephaelis ipecacuanha) with Agrobacterium rhizogenes. In: Planta Medica. 2003 ; Vol. 69, No. 11. pp. 1018-1023.
@article{f3a4ae8f8255487b875feafabf6d6373,
title = "Transformation of Ipecac (Cephaelis ipecacuanha) with Agrobacterium rhizogenes",
abstract = "Transformed root cultures of ipecac (Cephaelis ipecacuanha A. Richard), one of the recalcitrant woody plant species for Agrobacterium-mediated transformation, were established by co-culturing of in vitro petiole segments with Agrobacterium rhizogenes ATCC 15834. Southern blot analysis of the established roots revealed that only the TL-DNA was integrated into the plant genome without incorporation of the TR-DNA. The transformed roots grew slowly on phytohormone-free solid medium and adventitious shoots were regenerated after over 6 months of culture on HF, half-strength Murashige and Skoog (1/2 MS) medium in the dark. The individually separated transformed shoots developed into plantlets on phytohormone-free solid medium at 25°C under 16h/day light, and the plants demonstrated wider leaves, shorter internodes and vigorous root growth compared to non-transformed plants. Effects of basal media and auxins on the growth and the ipecac alkaloid production of the transformed roots were investigated either under light or in the dark. The roots cultured in the dark grew well in Gamborg B5 (B5) liquid medium containing 0.5mg/L IBA and yielded 112 mg/L of cephaeline and 14 mg/L emetine after 8 weeks of culture.",
keywords = "Agrobacterium rhizogenes, Cephaelis ipecacuanha, Ipecac, Isoquinoline alkaloids, Transformation",
author = "Kayo Yoshimatsu and Koichiro Shimomura and Mami Yamazaki and Kazuki Saito and Fumiyuki Kiuchi",
year = "2003",
month = "11",
doi = "10.1055/s-2003-45149",
language = "English",
volume = "69",
pages = "1018--1023",
journal = "Planta Medica",
issn = "0032-0943",
publisher = "Georg Thieme Verlag",
number = "11",

}

TY - JOUR

T1 - Transformation of Ipecac (Cephaelis ipecacuanha) with Agrobacterium rhizogenes

AU - Yoshimatsu, Kayo

AU - Shimomura, Koichiro

AU - Yamazaki, Mami

AU - Saito, Kazuki

AU - Kiuchi, Fumiyuki

PY - 2003/11

Y1 - 2003/11

N2 - Transformed root cultures of ipecac (Cephaelis ipecacuanha A. Richard), one of the recalcitrant woody plant species for Agrobacterium-mediated transformation, were established by co-culturing of in vitro petiole segments with Agrobacterium rhizogenes ATCC 15834. Southern blot analysis of the established roots revealed that only the TL-DNA was integrated into the plant genome without incorporation of the TR-DNA. The transformed roots grew slowly on phytohormone-free solid medium and adventitious shoots were regenerated after over 6 months of culture on HF, half-strength Murashige and Skoog (1/2 MS) medium in the dark. The individually separated transformed shoots developed into plantlets on phytohormone-free solid medium at 25°C under 16h/day light, and the plants demonstrated wider leaves, shorter internodes and vigorous root growth compared to non-transformed plants. Effects of basal media and auxins on the growth and the ipecac alkaloid production of the transformed roots were investigated either under light or in the dark. The roots cultured in the dark grew well in Gamborg B5 (B5) liquid medium containing 0.5mg/L IBA and yielded 112 mg/L of cephaeline and 14 mg/L emetine after 8 weeks of culture.

AB - Transformed root cultures of ipecac (Cephaelis ipecacuanha A. Richard), one of the recalcitrant woody plant species for Agrobacterium-mediated transformation, were established by co-culturing of in vitro petiole segments with Agrobacterium rhizogenes ATCC 15834. Southern blot analysis of the established roots revealed that only the TL-DNA was integrated into the plant genome without incorporation of the TR-DNA. The transformed roots grew slowly on phytohormone-free solid medium and adventitious shoots were regenerated after over 6 months of culture on HF, half-strength Murashige and Skoog (1/2 MS) medium in the dark. The individually separated transformed shoots developed into plantlets on phytohormone-free solid medium at 25°C under 16h/day light, and the plants demonstrated wider leaves, shorter internodes and vigorous root growth compared to non-transformed plants. Effects of basal media and auxins on the growth and the ipecac alkaloid production of the transformed roots were investigated either under light or in the dark. The roots cultured in the dark grew well in Gamborg B5 (B5) liquid medium containing 0.5mg/L IBA and yielded 112 mg/L of cephaeline and 14 mg/L emetine after 8 weeks of culture.

KW - Agrobacterium rhizogenes

KW - Cephaelis ipecacuanha

KW - Ipecac

KW - Isoquinoline alkaloids

KW - Transformation

UR - http://www.scopus.com/inward/record.url?scp=0346689997&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346689997&partnerID=8YFLogxK

U2 - 10.1055/s-2003-45149

DO - 10.1055/s-2003-45149

M3 - Article

VL - 69

SP - 1018

EP - 1023

JO - Planta Medica

JF - Planta Medica

SN - 0032-0943

IS - 11

ER -