Transformation of NIH/3T3 cells by DNA from a human hepatoma cell line with integrated hepatitis B virus DNA

Toshio Morizane; Tatsuya Nakamura; Hidetsugu Saito; Tetsu Watanabe; Yasutaka Inagaki; Itsurou Satoh; Kanji Tsuchimoto; Masaharu Tsuchiya

doi:10.1016/0277-5379(87)90010-1

Transformation of NIH/3T3 cells by DNA from a human hepatoma cell line with integrated hepatitis B virus DNA

Toshio Morizane, Tatsuya Nakamura, Hidetsugu Saito, Tetsu Watanabe, Yasutaka Inagaki, Itsurou Satoh, Kanji Tsuchimoto, Masaharu Tsuchiya

School of Pharmacy

Research output: Contribution to journal › Article

12 Citations (Scopus)

Abstract

We have studied by means of DNA-mediated gene transfer the transforming activity of the DNA of the human hepatoma cell line HCC-M, which contains genomes of hepatitis B virus (HBV) in integrated form. DNA from HCC-M induced transformed foci on transfection of NIH/3T3 cells. DNAs from primary transformants were capable of inducing secondary transformants. Most of the DNAs of these transformants were demonstrated to contain both human repetitive sequences and HBV DNA, indicating that the transformants had incorporated exogenous human DNA and HBV DNA as well. These results suggest that transformation occurs as the result of the transfer of oncogene which might be closely associated with HBV genome.

Original language	English
Pages (from-to)	163-165,167-169
Journal	European Journal of Cancer and Clinical Oncology
Volume	23
Issue number	2
DOIs	https://doi.org/10.1016/0277-5379(87)90010-1
Publication status	Published - 1987 Feb

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ASJC Scopus subject areas

Oncology

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Morizane, T., Nakamura, T., Saito, H., Watanabe, T., Inagaki, Y., Satoh, I., Tsuchimoto, K., & Tsuchiya, M. (1987). Transformation of NIH/3T3 cells by DNA from a human hepatoma cell line with integrated hepatitis B virus DNA. European Journal of Cancer and Clinical Oncology, 23(2), 163-165,167-169. https://doi.org/10.1016/0277-5379(87)90010-1

Transformation of NIH/3T3 cells by DNA from a human hepatoma cell line with integrated hepatitis B virus DNA. / Morizane, Toshio; Nakamura, Tatsuya; Saito, Hidetsugu; Watanabe, Tetsu; Inagaki, Yasutaka; Satoh, Itsurou; Tsuchimoto, Kanji; Tsuchiya, Masaharu.

In: European Journal of Cancer and Clinical Oncology, Vol. 23, No. 2, 02.1987, p. 163-165,167-169.

Research output: Contribution to journal › Article

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10.1016/0277-5379(87)90010-1