Abstract
We have studied by means of DNA-mediated gene transfer the transforming activity of the DNA of the human hepatoma cell line HCC-M, which contains genomes of hepatitis B virus (HBV) in integrated form. DNA from HCC-M induced transformed foci on transfection of NIH/3T3 cells. DNAs from primary transformants were capable of inducing secondary transformants. Most of the DNAs of these transformants were demonstrated to contain both human repetitive sequences and HBV DNA, indicating that the transformants had incorporated exogenous human DNA and HBV DNA as well. These results suggest that transformation occurs as the result of the transfer of oncogene which might be closely associated with HBV genome.
| Original language | English |
|---|---|
| Journal | European Journal of Cancer and Clinical Oncology |
| Volume | 23 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1987 |
Fingerprint
ASJC Scopus subject areas
- Oncology
Cite this
Transformation of NIH/3T3 cells by DNA from a human hepatoma cell line with integrated hepatitis B virus DNA. / Morizane, Toshio; Nakamura, Tatsuya; Saito, Hidetsugu; Watanabe, Tetsu; Inagaki, Yasutaka; Satoh, Itsurou; Tsuchimoto, Kanji; Tsuchiya, Masaharu.
In: European Journal of Cancer and Clinical Oncology, Vol. 23, No. 2, 1987.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transformation of NIH/3T3 cells by DNA from a human hepatoma cell line with integrated hepatitis B virus DNA
AU - Morizane, Toshio
AU - Nakamura, Tatsuya
AU - Saito, Hidetsugu
AU - Watanabe, Tetsu
AU - Inagaki, Yasutaka
AU - Satoh, Itsurou
AU - Tsuchimoto, Kanji
AU - Tsuchiya, Masaharu
PY - 1987
Y1 - 1987
N2 - We have studied by means of DNA-mediated gene transfer the transforming activity of the DNA of the human hepatoma cell line HCC-M, which contains genomes of hepatitis B virus (HBV) in integrated form. DNA from HCC-M induced transformed foci on transfection of NIH/3T3 cells. DNAs from primary transformants were capable of inducing secondary transformants. Most of the DNAs of these transformants were demonstrated to contain both human repetitive sequences and HBV DNA, indicating that the transformants had incorporated exogenous human DNA and HBV DNA as well. These results suggest that transformation occurs as the result of the transfer of oncogene which might be closely associated with HBV genome.
AB - We have studied by means of DNA-mediated gene transfer the transforming activity of the DNA of the human hepatoma cell line HCC-M, which contains genomes of hepatitis B virus (HBV) in integrated form. DNA from HCC-M induced transformed foci on transfection of NIH/3T3 cells. DNAs from primary transformants were capable of inducing secondary transformants. Most of the DNAs of these transformants were demonstrated to contain both human repetitive sequences and HBV DNA, indicating that the transformants had incorporated exogenous human DNA and HBV DNA as well. These results suggest that transformation occurs as the result of the transfer of oncogene which might be closely associated with HBV genome.
UR - http://www.scopus.com/inward/record.url?scp=0023070175&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023070175&partnerID=8YFLogxK
U2 - 10.1016/0277-5379(87)90010-1
DO - 10.1016/0277-5379(87)90010-1
M3 - Article
C2 - 2832175
AN - SCOPUS:0023070175
VL - 23
JO - European Journal of Cancer and Clinical Oncology
JF - European Journal of Cancer and Clinical Oncology
SN - 0277-5379
IS - 2
ER -