Transforming growth factor-β1 is responsible for maturation-dependent spontaneous apoptosis of cultured gastric pit cells

Shinji Tsutsumi, Wataru Tomisato, Tatsuya Hoshino, Tomofusa Tsuchiya, Tohru Mizushima

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In this study, we established a system of high concentration serum-dependent spontaneous apoptosis of guinea pig gastric pit cells in primary culture, which seems to mimic the spontaneous apoptosis of matured gastric pit cells at gastric surface in vivo. In addition to induction of the spontaneous apoptosis, cell growth was inhibited in the presence of 10% serum compared with 0.5% serum. Transforming growth factor-β1 (TGF-β1), which is known to cause both apoptosis and growth inhibition in mammalian cells, was present in serum of both fetal calf and guinea pig. The addition of recombinant TGF-β1 to the culture medium containing 0.5% fetal calf serum caused both induction of apoptosis and inhibition of cell growth. On the other hand, immunodepletion of TGF-β1 from fetal calf serum caused inability to induce both the spontaneous apoptosis and inhibition of cell growth. These data suggest that TGF-β1 is involved in the spontaneous apoptosis of guinea pig gastric pit cells in primary culture.

Original languageEnglish
Pages (from-to)402-411
Number of pages10
JournalExperimental Biology and Medicine
Volume227
Issue number6
Publication statusPublished - 2002 Jun
Externally publishedYes

Fingerprint

Transforming Growth Factors
Stomach
Apoptosis
Cell growth
Serum
Guinea Pigs
Primary Cell Culture
Growth
Culture Media
Cells

Keywords

  • Apoptosis
  • Caspases
  • Gastric mucosal cells
  • Transforming growth factor-β1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Transforming growth factor-β1 is responsible for maturation-dependent spontaneous apoptosis of cultured gastric pit cells. / Tsutsumi, Shinji; Tomisato, Wataru; Hoshino, Tatsuya; Tsuchiya, Tomofusa; Mizushima, Tohru.

In: Experimental Biology and Medicine, Vol. 227, No. 6, 06.2002, p. 402-411.

Research output: Contribution to journalArticle

Tsutsumi, S, Tomisato, W, Hoshino, T, Tsuchiya, T & Mizushima, T 2002, 'Transforming growth factor-β1 is responsible for maturation-dependent spontaneous apoptosis of cultured gastric pit cells', Experimental Biology and Medicine, vol. 227, no. 6, pp. 402-411.
Tsutsumi, Shinji ; Tomisato, Wataru ; Hoshino, Tatsuya ; Tsuchiya, Tomofusa ; Mizushima, Tohru. / Transforming growth factor-β1 is responsible for maturation-dependent spontaneous apoptosis of cultured gastric pit cells. In: Experimental Biology and Medicine. 2002 ; Vol. 227, No. 6. pp. 402-411.
@article{90f8cd181ccf4c9597ec7d398fa7a820,
title = "Transforming growth factor-β1 is responsible for maturation-dependent spontaneous apoptosis of cultured gastric pit cells",
abstract = "In this study, we established a system of high concentration serum-dependent spontaneous apoptosis of guinea pig gastric pit cells in primary culture, which seems to mimic the spontaneous apoptosis of matured gastric pit cells at gastric surface in vivo. In addition to induction of the spontaneous apoptosis, cell growth was inhibited in the presence of 10{\%} serum compared with 0.5{\%} serum. Transforming growth factor-β1 (TGF-β1), which is known to cause both apoptosis and growth inhibition in mammalian cells, was present in serum of both fetal calf and guinea pig. The addition of recombinant TGF-β1 to the culture medium containing 0.5{\%} fetal calf serum caused both induction of apoptosis and inhibition of cell growth. On the other hand, immunodepletion of TGF-β1 from fetal calf serum caused inability to induce both the spontaneous apoptosis and inhibition of cell growth. These data suggest that TGF-β1 is involved in the spontaneous apoptosis of guinea pig gastric pit cells in primary culture.",
keywords = "Apoptosis, Caspases, Gastric mucosal cells, Transforming growth factor-β1",
author = "Shinji Tsutsumi and Wataru Tomisato and Tatsuya Hoshino and Tomofusa Tsuchiya and Tohru Mizushima",
year = "2002",
month = "6",
language = "English",
volume = "227",
pages = "402--411",
journal = "Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)",
issn = "0037-9727",
publisher = "Society for Experimental Biology and Medicine",
number = "6",

}

TY - JOUR

T1 - Transforming growth factor-β1 is responsible for maturation-dependent spontaneous apoptosis of cultured gastric pit cells

AU - Tsutsumi, Shinji

AU - Tomisato, Wataru

AU - Hoshino, Tatsuya

AU - Tsuchiya, Tomofusa

AU - Mizushima, Tohru

PY - 2002/6

Y1 - 2002/6

N2 - In this study, we established a system of high concentration serum-dependent spontaneous apoptosis of guinea pig gastric pit cells in primary culture, which seems to mimic the spontaneous apoptosis of matured gastric pit cells at gastric surface in vivo. In addition to induction of the spontaneous apoptosis, cell growth was inhibited in the presence of 10% serum compared with 0.5% serum. Transforming growth factor-β1 (TGF-β1), which is known to cause both apoptosis and growth inhibition in mammalian cells, was present in serum of both fetal calf and guinea pig. The addition of recombinant TGF-β1 to the culture medium containing 0.5% fetal calf serum caused both induction of apoptosis and inhibition of cell growth. On the other hand, immunodepletion of TGF-β1 from fetal calf serum caused inability to induce both the spontaneous apoptosis and inhibition of cell growth. These data suggest that TGF-β1 is involved in the spontaneous apoptosis of guinea pig gastric pit cells in primary culture.

AB - In this study, we established a system of high concentration serum-dependent spontaneous apoptosis of guinea pig gastric pit cells in primary culture, which seems to mimic the spontaneous apoptosis of matured gastric pit cells at gastric surface in vivo. In addition to induction of the spontaneous apoptosis, cell growth was inhibited in the presence of 10% serum compared with 0.5% serum. Transforming growth factor-β1 (TGF-β1), which is known to cause both apoptosis and growth inhibition in mammalian cells, was present in serum of both fetal calf and guinea pig. The addition of recombinant TGF-β1 to the culture medium containing 0.5% fetal calf serum caused both induction of apoptosis and inhibition of cell growth. On the other hand, immunodepletion of TGF-β1 from fetal calf serum caused inability to induce both the spontaneous apoptosis and inhibition of cell growth. These data suggest that TGF-β1 is involved in the spontaneous apoptosis of guinea pig gastric pit cells in primary culture.

KW - Apoptosis

KW - Caspases

KW - Gastric mucosal cells

KW - Transforming growth factor-β1

UR - http://www.scopus.com/inward/record.url?scp=0036619461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036619461&partnerID=8YFLogxK

M3 - Article

C2 - 12037130

AN - SCOPUS:0036619461

VL - 227

SP - 402

EP - 411

JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)

JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)

SN - 0037-9727

IS - 6

ER -