Transforming growth factor-beta stimulates, and glucocorticoids and epidermal growth factor inhibit brain natriuretic peptide secretion from cultured human amnion cells

Hiroaki Itoh, Norimasa Sagawa, Masaaki Hasegawa, Kumiko Inamori, Hirohisa Ueda, Kyoko Kitagawa, Hidetaka Nanno, Yoshiyuki Ihara, Fuminori Kobayashi, Takahide Mori, Shin Ichi Suga, Takaaki Yoshimasa, Hiroshi Itoh, Kazuwa Nakao

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18 Citations (Scopus)

Abstract

We previously reported the massive secretion of brain natriuretic peptide (BNP) from human amnion cells and suggested the possible role of BNP in the maintenance of human pregnancy. In this study, to elucidate the regulatory mechanism of BNP secretion from amnion cells, we measured the BNP level in the culture medium of amnion cells by RIA after incubation in the presence of various substances. Among the agents examined, cortisol (1 × 10(-7) to 1 × 10(-6) mol/L), dexamethasone (1 × 10(-8) to 1 × 10(-6) mol/L), and epidermal growth factor (EGF; 2 × 10(-11) to 2 × 10(-8) mol/L) inhibited BNP secretion from the cultured amnion cells in a dose-dependent manner. By contrast, transforming growth factor-β (TGF β; 4 × 10(-11) to 4 × 10(-9) mol/L) caused a 3- to 5-fold increase in BNP secretion. TGF β-augmented BNP secretion was abolished by the addition of cortisol or EGF to the culture medium. Moreover, in this study, we revealed the presence of bioactive TGF β in human amniotic fluid (approximately 4 × 10(-10) mol/L). The present finding of tight regulation of BNP secretion from amnion cells by cortisol, EGF and TGF β, all at the concentrations physiologically present in human amniotic fluid, implies a physiological role of BNP secretion from amnion cells in the pregnant uterus.

Original languageEnglish
Pages (from-to)176-182
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume79
Issue number1
DOIs
Publication statusPublished - 1994 Jul
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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    Itoh, H., Sagawa, N., Hasegawa, M., Inamori, K., Ueda, H., Kitagawa, K., Nanno, H., Ihara, Y., Kobayashi, F., Mori, T., Suga, S. I., Yoshimasa, T., Itoh, H., & Nakao, K. (1994). Transforming growth factor-beta stimulates, and glucocorticoids and epidermal growth factor inhibit brain natriuretic peptide secretion from cultured human amnion cells. Journal of Clinical Endocrinology and Metabolism, 79(1), 176-182. https://doi.org/10.1210/jcem.79.1.8027223