Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo

Hidetoshi Hasuwa, Yuko Muro, Masahito Ikawa, Noriko Kato, Yoshihide Tsujimoto, Masaru Okabe

Research output: Contribution to journalComment/debate

69 Citations (Scopus)

Abstract

In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1-Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.

Original languageEnglish
Pages (from-to)105-107
Number of pages3
JournalExperimental Animals
Volume59
Issue number1
DOIs
Publication statusPublished - 2010 Mar 11
Externally publishedYes

Keywords

  • Live imaging
  • Oviduct
  • Sperm

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Fingerprint Dive into the research topics of 'Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo'. Together they form a unique fingerprint.

  • Cite this