Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo

Hidetoshi Hasuwa; Yuko Muro; Masahito Ikawa; Noriko Kato; Yoshihide Tsujimoto; Masaru Okabe

doi:10.1538/expanim.59.105

Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo

Hidetoshi Hasuwa, Yuko Muro, Masahito Ikawa, Noriko Kato, Yoshihide Tsujimoto, Masaru Okabe

Research output: Contribution to journal › Comment/debate › peer-review

101 Citations (Scopus)

Abstract

In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1-Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.

Original language	English
Pages (from-to)	105-107
Number of pages	3
Journal	Experimental Animals
Volume	59
Issue number	1
DOIs	https://doi.org/10.1538/expanim.59.105
Publication status	Published - 2010 Mar 11
Externally published	Yes

Keywords

Live imaging
Oviduct
Sperm

ASJC Scopus subject areas

Animal Science and Zoology
Biochemistry, Genetics and Molecular Biology(all)
veterinary(all)

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10.1538/expanim.59.105

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abstract = "In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1-Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.",

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T1 - Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo

AU - Hasuwa, Hidetoshi

AU - Muro, Yuko

AU - Ikawa, Masahito

AU - Kato, Noriko

AU - Tsujimoto, Yoshihide

AU - Okabe, Masaru

PY - 2010/3/11

Y1 - 2010/3/11

N2 - In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1-Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.

AB - In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1-Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.

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Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo

Abstract

Keywords

ASJC Scopus subject areas

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