Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells

Tomohiko Akiyama, Li Xin, Mayumi Oda, Alexei A. Sharov, Misa Amano, Yulan Piao, J. Scotty Cadet, Dawood B. Dudekula, Yong Qian, Weidong Wang, Shigeru Ko, Minoru Ko

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Mouse embryonic stem cells (mESCs) have a remarkable capacity to maintain normal genome stability and karyotype in culture. We previously showed that infrequent bursts of Zscan4 expression (Z4 events) are important for the maintenance of telomere length and genome stability in mESCs. However, the molecular details of Z4 events remain unclear. Here we show that Z4 events involve unexpected transcriptional derepression in heterochromatin regions that usually remain silent. During a Z4 event, we see rapid derepression and rerepression of heterochromatin leading to a burst of transcription that coincides with transient histone hyperacetylation and DNA demethylation, clustering of pericentromeric heterochromatin around the nucleolus, and accumulation of activating and repressive chromatin remodelling complexes. This heterochromatin-based transcriptional activity suggests that mESCs may maintain their extraordinary genome stability at least in part by transiently resetting their heterochromatin.

Original languageEnglish
Pages (from-to)307-318
Number of pages12
JournalDNA Research
Volume22
Issue number5
DOIs
Publication statusPublished - 2015 May 6

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Heterochromatin
Genomic Instability
Telomere Homeostasis
Chromatin Assembly and Disassembly
Karyotype
Histones
Cluster Analysis
Mouse Embryonic Stem Cells
DNA

Keywords

  • embryonic stem cells
  • heterochromatin
  • pericentromere

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells. / Akiyama, Tomohiko; Xin, Li; Oda, Mayumi; Sharov, Alexei A.; Amano, Misa; Piao, Yulan; Cadet, J. Scotty; Dudekula, Dawood B.; Qian, Yong; Wang, Weidong; Ko, Shigeru; Ko, Minoru.

In: DNA Research, Vol. 22, No. 5, 06.05.2015, p. 307-318.

Research output: Contribution to journalArticle

Akiyama, Tomohiko ; Xin, Li ; Oda, Mayumi ; Sharov, Alexei A. ; Amano, Misa ; Piao, Yulan ; Cadet, J. Scotty ; Dudekula, Dawood B. ; Qian, Yong ; Wang, Weidong ; Ko, Shigeru ; Ko, Minoru. / Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells. In: DNA Research. 2015 ; Vol. 22, No. 5. pp. 307-318.
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