Transient forebrain ischemia induces expression of serine/threonine protein phosphatase 1 mRNA in the vulnerable regions of gerbil brain

Takashi Horiguchi, Hiroshi Shima, Sadao Suga, Masahiro Ogino, Katsuyoshi Shimizu, Shigeo Toya, Minako Nagao, Takeshi Kawase

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13 Citations (Scopus)

Abstract

Apoptosis is thought to be implicated in delayed neuronal cell death following transient forebrain ischemia. Recently, apoptosis in neurons induced by an inhibitor of serine/threonine (ser/thr) protein phosphatases (PPs) has been reported. In this study, we investigated the effect of transient forebrain ischemia on the expression of ser/thr PPs in the brain of Mongolian gerbils. At 24 h after 5-min bilateral carotid artery occlusion, Northern blotting analysis revealed the increase of PP1 mRNA expression in the vulnerable CA1 region of the hippocampus and striatum, but not in the cortex and CA3 region. In contrast, the protein level of PP1 detected by Western blotting analysis decreased in all regions. We conclude that the inhibition in PPs expression in the vulnerable regions may affect cell death after transient forebrain ischemia.

Original languageEnglish
Pages (from-to)115-118
Number of pages4
JournalNeuroscience Letters
Volume325
Issue number2
DOIs
Publication statusPublished - 2002 Jun 7

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Keywords

  • Delayed neuronal death
  • Global ischemia
  • Protein phosphatase
  • Protein synthesis
  • Vulnerability

ASJC Scopus subject areas

  • Neuroscience(all)

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