Transient forebrain ischemia induces expression of serine/threonine protein phosphatase 1 mRNA in the vulnerable regions of gerbil brain

Takashi Horiguchi; Hiroshi Shima; Sadao Suga; Masahiro Ogino; Katsuyoshi Shimizu; Shigeo Toya; Minako Nagao; Takeshi Kawase

doi:10.1016/S0304-3940(02)00244-6

Transient forebrain ischemia induces expression of serine/threonine protein phosphatase 1 mRNA in the vulnerable regions of gerbil brain

Takashi Horiguchi, Hiroshi Shima, Sadao Suga, Masahiro Ogino, Katsuyoshi Shimizu, Shigeo Toya, Minako Nagao, Takeshi Kawase

Department of Neurosurgery

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Apoptosis is thought to be implicated in delayed neuronal cell death following transient forebrain ischemia. Recently, apoptosis in neurons induced by an inhibitor of serine/threonine (ser/thr) protein phosphatases (PPs) has been reported. In this study, we investigated the effect of transient forebrain ischemia on the expression of ser/thr PPs in the brain of Mongolian gerbils. At 24 h after 5-min bilateral carotid artery occlusion, Northern blotting analysis revealed the increase of PP1 mRNA expression in the vulnerable CA1 region of the hippocampus and striatum, but not in the cortex and CA3 region. In contrast, the protein level of PP1 detected by Western blotting analysis decreased in all regions. We conclude that the inhibition in PPs expression in the vulnerable regions may affect cell death after transient forebrain ischemia.

Original language	English
Pages (from-to)	115-118
Number of pages	4
Journal	Neuroscience Letters
Volume	325
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(02)00244-6
Publication status	Published - 2002 Jun 7

Keywords

Delayed neuronal death
Global ischemia
Protein phosphatase
Protein synthesis
Vulnerability

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(02)00244-6

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title = "Transient forebrain ischemia induces expression of serine/threonine protein phosphatase 1 mRNA in the vulnerable regions of gerbil brain",

abstract = "Apoptosis is thought to be implicated in delayed neuronal cell death following transient forebrain ischemia. Recently, apoptosis in neurons induced by an inhibitor of serine/threonine (ser/thr) protein phosphatases (PPs) has been reported. In this study, we investigated the effect of transient forebrain ischemia on the expression of ser/thr PPs in the brain of Mongolian gerbils. At 24 h after 5-min bilateral carotid artery occlusion, Northern blotting analysis revealed the increase of PP1 mRNA expression in the vulnerable CA1 region of the hippocampus and striatum, but not in the cortex and CA3 region. In contrast, the protein level of PP1 detected by Western blotting analysis decreased in all regions. We conclude that the inhibition in PPs expression in the vulnerable regions may affect cell death after transient forebrain ischemia.",

keywords = "Delayed neuronal death, Global ischemia, Protein phosphatase, Protein synthesis, Vulnerability",

author = "Takashi Horiguchi and Hiroshi Shima and Sadao Suga and Masahiro Ogino and Katsuyoshi Shimizu and Shigeo Toya and Minako Nagao and Takeshi Kawase",

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T1 - Transient forebrain ischemia induces expression of serine/threonine protein phosphatase 1 mRNA in the vulnerable regions of gerbil brain

AU - Horiguchi, Takashi

AU - Shima, Hiroshi

AU - Suga, Sadao

AU - Ogino, Masahiro

AU - Shimizu, Katsuyoshi

AU - Toya, Shigeo

AU - Nagao, Minako

AU - Kawase, Takeshi

PY - 2002/6/7

Y1 - 2002/6/7

N2 - Apoptosis is thought to be implicated in delayed neuronal cell death following transient forebrain ischemia. Recently, apoptosis in neurons induced by an inhibitor of serine/threonine (ser/thr) protein phosphatases (PPs) has been reported. In this study, we investigated the effect of transient forebrain ischemia on the expression of ser/thr PPs in the brain of Mongolian gerbils. At 24 h after 5-min bilateral carotid artery occlusion, Northern blotting analysis revealed the increase of PP1 mRNA expression in the vulnerable CA1 region of the hippocampus and striatum, but not in the cortex and CA3 region. In contrast, the protein level of PP1 detected by Western blotting analysis decreased in all regions. We conclude that the inhibition in PPs expression in the vulnerable regions may affect cell death after transient forebrain ischemia.

AB - Apoptosis is thought to be implicated in delayed neuronal cell death following transient forebrain ischemia. Recently, apoptosis in neurons induced by an inhibitor of serine/threonine (ser/thr) protein phosphatases (PPs) has been reported. In this study, we investigated the effect of transient forebrain ischemia on the expression of ser/thr PPs in the brain of Mongolian gerbils. At 24 h after 5-min bilateral carotid artery occlusion, Northern blotting analysis revealed the increase of PP1 mRNA expression in the vulnerable CA1 region of the hippocampus and striatum, but not in the cortex and CA3 region. In contrast, the protein level of PP1 detected by Western blotting analysis decreased in all regions. We conclude that the inhibition in PPs expression in the vulnerable regions may affect cell death after transient forebrain ischemia.

KW - Delayed neuronal death

KW - Global ischemia

KW - Protein phosphatase

KW - Protein synthesis

KW - Vulnerability

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JF - Neuroscience Letters

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ER -

Transient forebrain ischemia induces expression of serine/threonine protein phosphatase 1 mRNA in the vulnerable regions of gerbil brain

Abstract

Keywords

ASJC Scopus subject areas

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