Transient inhibition of BMP signaling by Noggin induces cardiomyocyte differentiation of mouse embryonic stem cells

Shinsuke Yuasa, Yuji Itabashi, Uichi Koshimizu, Tomofumi Tanaka, Keijiro Sugimura, Masayoshi Kinoshita, Fumiyuki Hattori, Shin Ichi Fukami, Takuya Shimazaki, Hideyuki Okano, Satoshi Ogawa, Keiichi Fukuda

Research output: Contribution to journalArticle

234 Citations (Scopus)

Abstract

Embryonic stem (ES) cells are a promising source of cardiomyocytes, but clinical application of ES cells has been hindered by the lack of reliable selective differentiation methods. Differentiation into any lineage is partly dependent on the regulatory mechanisms of normal early development. Although several signals, including bone morphogenetic protein (BMP), Wnt and FGF, are involved in heart development, scarce evidence is available about the exact signals that mediate cardiomyocyte differentiation. While investigating the involvement of BMP signaling in early heart formation in the mouse, we found that the BMP antagonist Noggin is transiently but strongly expressed in the heart-forming region during gastrulation and acts at the level of induction of mesendoderm to establish conditions conducive to cardiogenesis. We applied this finding to develop an effective protocol for obtaining cardiomyocytes from mouse ES cells by inhibition of BMP signaling.

Original languageEnglish
Pages (from-to)607-611
Number of pages5
JournalNature Biotechnology
Volume23
Issue number5
DOIs
Publication statusPublished - 2005

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Bone Morphogenetic Proteins
Stem cells
Cardiac Myocytes
Bone
Proteins
Embryonic Stem Cells
Gastrulation
Mouse Embryonic Stem Cells

ASJC Scopus subject areas

  • Microbiology

Cite this

Transient inhibition of BMP signaling by Noggin induces cardiomyocyte differentiation of mouse embryonic stem cells. / Yuasa, Shinsuke; Itabashi, Yuji; Koshimizu, Uichi; Tanaka, Tomofumi; Sugimura, Keijiro; Kinoshita, Masayoshi; Hattori, Fumiyuki; Fukami, Shin Ichi; Shimazaki, Takuya; Okano, Hideyuki; Ogawa, Satoshi; Fukuda, Keiichi.

In: Nature Biotechnology, Vol. 23, No. 5, 2005, p. 607-611.

Research output: Contribution to journalArticle

Yuasa, Shinsuke ; Itabashi, Yuji ; Koshimizu, Uichi ; Tanaka, Tomofumi ; Sugimura, Keijiro ; Kinoshita, Masayoshi ; Hattori, Fumiyuki ; Fukami, Shin Ichi ; Shimazaki, Takuya ; Okano, Hideyuki ; Ogawa, Satoshi ; Fukuda, Keiichi. / Transient inhibition of BMP signaling by Noggin induces cardiomyocyte differentiation of mouse embryonic stem cells. In: Nature Biotechnology. 2005 ; Vol. 23, No. 5. pp. 607-611.
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