Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells

Masahiro Yamamoto, Mitsue Nishiyama, Seiichi Iizuka, Shigeaki Suzuki, Norihiro Suzuki, Sadakazu Aiso, Jin Nakahara

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

AIM To investigate the possible involvement of transient receptor potential vanilloid 1 (TRPV1) in maturation of enteric glial cells (EGCs). METHODS Immunohistochemical and immunocytochemical techniques were used to analyze EGC markers in myenteric plexus (MP) as well as cultured MP cells and EGCs using TRPV1 knockout (KO) mice. RESULTS We detected TRPV1-immunoreactive signals in EGC in the MP of wild-type (WT) but not KO mice. Expression of glial fibrillary acidic protein (GFAP) immunoreactive signals was lower at postnatal day (PD) 6 in KO mice, though the difference was not clear at PD 13 and PD 21. When MP cells were isolated and cultured from isolated longitudinal muscle-MP preparation from WT and KO mice, the yield of KO EGC was lower than that of WT EGC, while the yield of KO and WT smooth muscle cells showed no difference. Addition of BCTC, a TRPV1 antagonist, to enriched EGC culture resulted in a decrease in the protein ratio of GFAP to S100B, another EGC/astrocyte-specific marker. CONCLUSION These results address the possibility that TRPV1 may be involved in the maturation of EGC, though further studies are necessary to validate this possibility.

Original languageEnglish
Pages (from-to)9752-9764
Number of pages13
JournalWorld Journal of Gastroenterology
Volume22
Issue number44
DOIs
Publication statusPublished - 2016

Fingerprint

Neuroglia
Myenteric Plexus
Knockout Mice
Glial Fibrillary Acidic Protein
vanilloid receptor subtype 1
Astrocytes
Smooth Muscle Myocytes
Cell Culture Techniques
Muscles

Keywords

  • Enteric glial cells; Enteric nervous system; Glial fibrillary acidic protein; S100B; Smooth muscle cells

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells. / Yamamoto, Masahiro; Nishiyama, Mitsue; Iizuka, Seiichi; Suzuki, Shigeaki; Suzuki, Norihiro; Aiso, Sadakazu; Nakahara, Jin.

In: World Journal of Gastroenterology, Vol. 22, No. 44, 2016, p. 9752-9764.

Research output: Contribution to journalArticle

Yamamoto, Masahiro ; Nishiyama, Mitsue ; Iizuka, Seiichi ; Suzuki, Shigeaki ; Suzuki, Norihiro ; Aiso, Sadakazu ; Nakahara, Jin. / Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells. In: World Journal of Gastroenterology. 2016 ; Vol. 22, No. 44. pp. 9752-9764.
@article{b9a7713e903748d18f0909c3db571f8d,
title = "Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells",
abstract = "AIM To investigate the possible involvement of transient receptor potential vanilloid 1 (TRPV1) in maturation of enteric glial cells (EGCs). METHODS Immunohistochemical and immunocytochemical techniques were used to analyze EGC markers in myenteric plexus (MP) as well as cultured MP cells and EGCs using TRPV1 knockout (KO) mice. RESULTS We detected TRPV1-immunoreactive signals in EGC in the MP of wild-type (WT) but not KO mice. Expression of glial fibrillary acidic protein (GFAP) immunoreactive signals was lower at postnatal day (PD) 6 in KO mice, though the difference was not clear at PD 13 and PD 21. When MP cells were isolated and cultured from isolated longitudinal muscle-MP preparation from WT and KO mice, the yield of KO EGC was lower than that of WT EGC, while the yield of KO and WT smooth muscle cells showed no difference. Addition of BCTC, a TRPV1 antagonist, to enriched EGC culture resulted in a decrease in the protein ratio of GFAP to S100B, another EGC/astrocyte-specific marker. CONCLUSION These results address the possibility that TRPV1 may be involved in the maturation of EGC, though further studies are necessary to validate this possibility.",
keywords = "Enteric glial cells; Enteric nervous system; Glial fibrillary acidic protein; S100B; Smooth muscle cells",
author = "Masahiro Yamamoto and Mitsue Nishiyama and Seiichi Iizuka and Shigeaki Suzuki and Norihiro Suzuki and Sadakazu Aiso and Jin Nakahara",
year = "2016",
doi = "10.3748/wjg.v22.i44.9752",
language = "English",
volume = "22",
pages = "9752--9764",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "44",

}

TY - JOUR

T1 - Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells

AU - Yamamoto, Masahiro

AU - Nishiyama, Mitsue

AU - Iizuka, Seiichi

AU - Suzuki, Shigeaki

AU - Suzuki, Norihiro

AU - Aiso, Sadakazu

AU - Nakahara, Jin

PY - 2016

Y1 - 2016

N2 - AIM To investigate the possible involvement of transient receptor potential vanilloid 1 (TRPV1) in maturation of enteric glial cells (EGCs). METHODS Immunohistochemical and immunocytochemical techniques were used to analyze EGC markers in myenteric plexus (MP) as well as cultured MP cells and EGCs using TRPV1 knockout (KO) mice. RESULTS We detected TRPV1-immunoreactive signals in EGC in the MP of wild-type (WT) but not KO mice. Expression of glial fibrillary acidic protein (GFAP) immunoreactive signals was lower at postnatal day (PD) 6 in KO mice, though the difference was not clear at PD 13 and PD 21. When MP cells were isolated and cultured from isolated longitudinal muscle-MP preparation from WT and KO mice, the yield of KO EGC was lower than that of WT EGC, while the yield of KO and WT smooth muscle cells showed no difference. Addition of BCTC, a TRPV1 antagonist, to enriched EGC culture resulted in a decrease in the protein ratio of GFAP to S100B, another EGC/astrocyte-specific marker. CONCLUSION These results address the possibility that TRPV1 may be involved in the maturation of EGC, though further studies are necessary to validate this possibility.

AB - AIM To investigate the possible involvement of transient receptor potential vanilloid 1 (TRPV1) in maturation of enteric glial cells (EGCs). METHODS Immunohistochemical and immunocytochemical techniques were used to analyze EGC markers in myenteric plexus (MP) as well as cultured MP cells and EGCs using TRPV1 knockout (KO) mice. RESULTS We detected TRPV1-immunoreactive signals in EGC in the MP of wild-type (WT) but not KO mice. Expression of glial fibrillary acidic protein (GFAP) immunoreactive signals was lower at postnatal day (PD) 6 in KO mice, though the difference was not clear at PD 13 and PD 21. When MP cells were isolated and cultured from isolated longitudinal muscle-MP preparation from WT and KO mice, the yield of KO EGC was lower than that of WT EGC, while the yield of KO and WT smooth muscle cells showed no difference. Addition of BCTC, a TRPV1 antagonist, to enriched EGC culture resulted in a decrease in the protein ratio of GFAP to S100B, another EGC/astrocyte-specific marker. CONCLUSION These results address the possibility that TRPV1 may be involved in the maturation of EGC, though further studies are necessary to validate this possibility.

KW - Enteric glial cells; Enteric nervous system; Glial fibrillary acidic protein; S100B; Smooth muscle cells

UR - http://www.scopus.com/inward/record.url?scp=84997428966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84997428966&partnerID=8YFLogxK

U2 - 10.3748/wjg.v22.i44.9752

DO - 10.3748/wjg.v22.i44.9752

M3 - Article

VL - 22

SP - 9752

EP - 9764

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 44

ER -