Transient relaxation of plasmid DNA in Escherichia coli by fluoroquinolones

Miki Matsuo; Yoshihisa Ohtsuka; Kazuhiro Kataoka; Tohru Mizushima; Kazuhisa Sekimizu

doi:10.1111/j.2042-7158.1996.tb06018.x

Transient relaxation of plasmid DNA in Escherichia coli by fluoroquinolones

Miki Matsuo, Yoshihisa Ohtsuka, Kazuhiro Kataoka, Tohru Mizushima, Kazuhisa Sekimizu

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We examined the influence of fluoroquinolones (norfloxacin, enoxacin, ofloxacin, levofloxacin, and sparfloxacin) on DNA supercoiling of plasmids in Escherichia coli cells by analysis with agarose gel electrophoresis in the presence of chloroquine. All the fluoroquinolones tested immediately induced DNA relaxation. The relaxed DNA was re-supercoiled, and the process was sensitive to chloramphenicol, suggesting that newly synthesized proteins participate in the reaction. The concentrations of fluoroquinolones required for DNA relaxation were much higher than those required for cell killing. The bactericidal effect of fluoroquinolones is apparently related to mechanisms other than DNA relaxation.

Original language	English
Pages (from-to)	985-987
Number of pages	3
Journal	Journal of Pharmacy and Pharmacology
Volume	48
Issue number	9
DOIs	https://doi.org/10.1111/j.2042-7158.1996.tb06018.x
Publication status	Published - 1996 Sept

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

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10.1111/j.2042-7158.1996.tb06018.x

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abstract = "We examined the influence of fluoroquinolones (norfloxacin, enoxacin, ofloxacin, levofloxacin, and sparfloxacin) on DNA supercoiling of plasmids in Escherichia coli cells by analysis with agarose gel electrophoresis in the presence of chloroquine. All the fluoroquinolones tested immediately induced DNA relaxation. The relaxed DNA was re-supercoiled, and the process was sensitive to chloramphenicol, suggesting that newly synthesized proteins participate in the reaction. The concentrations of fluoroquinolones required for DNA relaxation were much higher than those required for cell killing. The bactericidal effect of fluoroquinolones is apparently related to mechanisms other than DNA relaxation.",

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AU - Ohtsuka, Yoshihisa

AU - Kataoka, Kazuhiro

AU - Mizushima, Tohru

AU - Sekimizu, Kazuhisa

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AB - We examined the influence of fluoroquinolones (norfloxacin, enoxacin, ofloxacin, levofloxacin, and sparfloxacin) on DNA supercoiling of plasmids in Escherichia coli cells by analysis with agarose gel electrophoresis in the presence of chloroquine. All the fluoroquinolones tested immediately induced DNA relaxation. The relaxed DNA was re-supercoiled, and the process was sensitive to chloramphenicol, suggesting that newly synthesized proteins participate in the reaction. The concentrations of fluoroquinolones required for DNA relaxation were much higher than those required for cell killing. The bactericidal effect of fluoroquinolones is apparently related to mechanisms other than DNA relaxation.

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Transient relaxation of plasmid DNA in Escherichia coli by fluoroquinolones

Abstract

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