Translational repression determines a neuronal potential in Drosophila asymmetric cell division

Masataka Okabe, Takao Imai, Mitsuhiko Kurusu, Yasushi Hiromi, Hideyuki Okano

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Asymmetric cell division is a fundamental strategy for generating cellular diversity during animal development. Daughter cells manifest asymmetry in their differential gene expression. Transcriptional regulation of this process has been the focus of many studies, whereas cell-type-specific 'translational' regulation has been considered to have a more minor role. During sensory organ development in Drosophila, Notch signalling directs the asymmetry between neuronal and non-neuronal lineages, and a zinc-finger transcriptional repressor Tramtrack69 (TTK69) acts downstream of Notch as a determinant of non-neuronal identity. Here we show that repression of TTK69 protein expression in the neuronal lineage occurs translationally rather than transcriptionally. This translational repression is achieved by a direct interaction between cis-acting sequences in the 3′ untranslated region of ttk69 messenger RNA and its trans-acting repressor, the RNA-binding protein Musashi (MSI). Although msi can act downstream of Notch, Notch signalling does not affect MSI expression. Thus, Notch signalling is likely to regulate MSI activity rather than its expression. Our results define cell-type-specific translational control of ttk69 by MSI as a downstream event of Notch signalling in asymmetric cell division.

Original languageEnglish
Pages (from-to)94-98
Number of pages5
JournalNature
Volume411
Issue number6833
DOIs
Publication statusPublished - 2001 May 3

Fingerprint

Asymmetric Cell Division
Drosophila
RNA-Binding Proteins
Zinc Fingers
3' Untranslated Regions
Gene Expression
Messenger RNA
Proteins

ASJC Scopus subject areas

  • General

Cite this

Translational repression determines a neuronal potential in Drosophila asymmetric cell division. / Okabe, Masataka; Imai, Takao; Kurusu, Mitsuhiko; Hiromi, Yasushi; Okano, Hideyuki.

In: Nature, Vol. 411, No. 6833, 03.05.2001, p. 94-98.

Research output: Contribution to journalArticle

Okabe, Masataka ; Imai, Takao ; Kurusu, Mitsuhiko ; Hiromi, Yasushi ; Okano, Hideyuki. / Translational repression determines a neuronal potential in Drosophila asymmetric cell division. In: Nature. 2001 ; Vol. 411, No. 6833. pp. 94-98.
@article{9e8b0a7fff0c4d16937ccd767565bc8c,
title = "Translational repression determines a neuronal potential in Drosophila asymmetric cell division",
abstract = "Asymmetric cell division is a fundamental strategy for generating cellular diversity during animal development. Daughter cells manifest asymmetry in their differential gene expression. Transcriptional regulation of this process has been the focus of many studies, whereas cell-type-specific 'translational' regulation has been considered to have a more minor role. During sensory organ development in Drosophila, Notch signalling directs the asymmetry between neuronal and non-neuronal lineages, and a zinc-finger transcriptional repressor Tramtrack69 (TTK69) acts downstream of Notch as a determinant of non-neuronal identity. Here we show that repression of TTK69 protein expression in the neuronal lineage occurs translationally rather than transcriptionally. This translational repression is achieved by a direct interaction between cis-acting sequences in the 3′ untranslated region of ttk69 messenger RNA and its trans-acting repressor, the RNA-binding protein Musashi (MSI). Although msi can act downstream of Notch, Notch signalling does not affect MSI expression. Thus, Notch signalling is likely to regulate MSI activity rather than its expression. Our results define cell-type-specific translational control of ttk69 by MSI as a downstream event of Notch signalling in asymmetric cell division.",
author = "Masataka Okabe and Takao Imai and Mitsuhiko Kurusu and Yasushi Hiromi and Hideyuki Okano",
year = "2001",
month = "5",
day = "3",
doi = "10.1038/35075094",
language = "English",
volume = "411",
pages = "94--98",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "6833",

}

TY - JOUR

T1 - Translational repression determines a neuronal potential in Drosophila asymmetric cell division

AU - Okabe, Masataka

AU - Imai, Takao

AU - Kurusu, Mitsuhiko

AU - Hiromi, Yasushi

AU - Okano, Hideyuki

PY - 2001/5/3

Y1 - 2001/5/3

N2 - Asymmetric cell division is a fundamental strategy for generating cellular diversity during animal development. Daughter cells manifest asymmetry in their differential gene expression. Transcriptional regulation of this process has been the focus of many studies, whereas cell-type-specific 'translational' regulation has been considered to have a more minor role. During sensory organ development in Drosophila, Notch signalling directs the asymmetry between neuronal and non-neuronal lineages, and a zinc-finger transcriptional repressor Tramtrack69 (TTK69) acts downstream of Notch as a determinant of non-neuronal identity. Here we show that repression of TTK69 protein expression in the neuronal lineage occurs translationally rather than transcriptionally. This translational repression is achieved by a direct interaction between cis-acting sequences in the 3′ untranslated region of ttk69 messenger RNA and its trans-acting repressor, the RNA-binding protein Musashi (MSI). Although msi can act downstream of Notch, Notch signalling does not affect MSI expression. Thus, Notch signalling is likely to regulate MSI activity rather than its expression. Our results define cell-type-specific translational control of ttk69 by MSI as a downstream event of Notch signalling in asymmetric cell division.

AB - Asymmetric cell division is a fundamental strategy for generating cellular diversity during animal development. Daughter cells manifest asymmetry in their differential gene expression. Transcriptional regulation of this process has been the focus of many studies, whereas cell-type-specific 'translational' regulation has been considered to have a more minor role. During sensory organ development in Drosophila, Notch signalling directs the asymmetry between neuronal and non-neuronal lineages, and a zinc-finger transcriptional repressor Tramtrack69 (TTK69) acts downstream of Notch as a determinant of non-neuronal identity. Here we show that repression of TTK69 protein expression in the neuronal lineage occurs translationally rather than transcriptionally. This translational repression is achieved by a direct interaction between cis-acting sequences in the 3′ untranslated region of ttk69 messenger RNA and its trans-acting repressor, the RNA-binding protein Musashi (MSI). Although msi can act downstream of Notch, Notch signalling does not affect MSI expression. Thus, Notch signalling is likely to regulate MSI activity rather than its expression. Our results define cell-type-specific translational control of ttk69 by MSI as a downstream event of Notch signalling in asymmetric cell division.

UR - http://www.scopus.com/inward/record.url?scp=0035799579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035799579&partnerID=8YFLogxK

U2 - 10.1038/35075094

DO - 10.1038/35075094

M3 - Article

C2 - 11333984

AN - SCOPUS:0035799579

VL - 411

SP - 94

EP - 98

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 6833

ER -