Translocation of the Csk homologous kinase (Chk/Hyl) controls activity of CD36-anchored Lyn tyrosine kinase in thrombin-stimulated platelets

Atsushi Hirao, Isao Hamaguchi, Toshio Suda, Naoto Yamaguchi

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Chk/Hyl is a recently isolated non-receptor tyrosine kinase with greatest homology to a ubiquitous negative regulator of Src family kinases, Csk. To understand the significance of co-expression of Chk and Csk in platelets, we examined the subcellular localization of each protein. Chk, but not Csk, was completely translocated from the Triton X-100-soluble to the Triton X-100-insoluble cytoskeletal fraction within 10 s of thrombin stimulation. Chk and Lyn, but not Csk and c-Src, co-fractionated in the higher density lysate fractions of resting platelets, with Chk being found to localize close to CD36 (membrane glycoprotein IV)-anchored Lyn. The kinase activity of co-fractionated Lyn was suppressed 3-fold. In vitro phosphorylation assays showed that Chk suppressed Lyn activity by phosphorylating its C-terminal negative regulatory tyrosine. Upon stimulation of platelets with thrombin, the rapid and complete translocation of Chk away from Lyn caused concomitant activation of Lyn. This activation was accompanied by dephosphorylation of Lyn at its C-terminal negative regulatory tyrosine in cooperation with a protein tyrosine phosphatase. These results suggest that Chk, but not Csk, may function as a translocation-controlled negative regulator of CD36-anchored Lyn in thrombin-induced platelet activation.

Original languageEnglish
Pages (from-to)2342-2351
Number of pages10
JournalEMBO Journal
Volume16
Issue number9
DOIs
Publication statusPublished - 1997 May 1

Fingerprint

Platelets
Thrombin
Protein-Tyrosine Kinases
Phosphotransferases
Blood Platelets
Octoxynol
Tyrosine
Chemical activation
Protein Tyrosine Phosphatases
src-Family Kinases
Membrane Glycoproteins
Platelet Activation
Phosphorylation
Assays
Proteins

Keywords

  • CD36-anchored Lyn kinase
  • Csk homologous kinase
  • Platelet activation
  • Translocation
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Translocation of the Csk homologous kinase (Chk/Hyl) controls activity of CD36-anchored Lyn tyrosine kinase in thrombin-stimulated platelets. / Hirao, Atsushi; Hamaguchi, Isao; Suda, Toshio; Yamaguchi, Naoto.

In: EMBO Journal, Vol. 16, No. 9, 01.05.1997, p. 2342-2351.

Research output: Contribution to journalArticle

Hirao, Atsushi ; Hamaguchi, Isao ; Suda, Toshio ; Yamaguchi, Naoto. / Translocation of the Csk homologous kinase (Chk/Hyl) controls activity of CD36-anchored Lyn tyrosine kinase in thrombin-stimulated platelets. In: EMBO Journal. 1997 ; Vol. 16, No. 9. pp. 2342-2351.
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