Transmural pressure induces IL-6 secretion by intestinal epithelial cells

H. Kishikawa, S. Miura, H. Yoshida, M. Hirokawa, H. Nakamizo, H. Higuchi, M. Adachi, R. C. Nakatsumi, H. Suzuki, Hidetsugu Saito, H. Ishii

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Abstract

Although intestinal epithelial cells (IECs) are known as an important source for IL-6, it is not known whether mechanical forces affect IL-6 production. We investigated how transmural pressure modulates IL-6 synthesis and activation of transcription factors in IECs. Pressure was loaded onto IEC-18 cells by introducing compressed helium gas into the cell culture flask for 1-48 h. IL-6 release into the culture media was determined by cell proliferation bioassay using an IL-6-dependent mouse hybridoma cell line (7TD1). Exposure to pressure (80mmHg) significantly enhanced IL-6 release into the culture media from IEC-18 cells at 12 h. Under control conditions, IL-6 secretion was directed to the basolateral side, but after exposure to pressure IL-6 secretion was increased in both the apical and basolateral sides. A nuclear factor kappa B (NF-κB) decoy reversed completely the pressure-induced increase of IL-6 secretion by IEC-18 cells. Pressure treatment enhanced IL-6 mRNA expression in IECs within 6 h. Pressure loading significantly enhanced the activation of both NF-κB and NF-IL-6 from 1 h in the nuclear protein of IEC-18 cells as assessed by the electrophoretic mobility shift assay using FITC-conjugated specific primers. Increased phosphorylation of I-kappa B was also demonstrated in the cytosol of IEC cells within 1 h by Western blot analysis. These results suggest a possible role for pressure loading in immune modulation of the intestinal mucosa by the stimulation of IL-6 release from intestinal epithelial cells.

Original languageEnglish
Pages (from-to)86-91
Number of pages6
JournalClinical and Experimental Immunology
Volume129
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Intestinal Secretions
Interleukin-6
Epithelial Cells
Pressure
NF-kappa B
I-kappa B Proteins
Culture Media
Helium
Fluorescein-5-isothiocyanate
Hybridomas
Electrophoretic Mobility Shift Assay
Intestinal Mucosa
Nuclear Proteins
Interleukin-1
Biological Assay
Cytosol
Transcription Factors
Cell Culture Techniques
Gases
Western Blotting

Keywords

  • IL-6
  • Intestinal epithelial cells
  • NF-κB
  • NF-IL-6
  • Transmural pressure

ASJC Scopus subject areas

  • Immunology

Cite this

Kishikawa, H., Miura, S., Yoshida, H., Hirokawa, M., Nakamizo, H., Higuchi, H., ... Ishii, H. (2002). Transmural pressure induces IL-6 secretion by intestinal epithelial cells. Clinical and Experimental Immunology, 129(1), 86-91. https://doi.org/10.1046/j.1365-2249.2002.01895.x

Transmural pressure induces IL-6 secretion by intestinal epithelial cells. / Kishikawa, H.; Miura, S.; Yoshida, H.; Hirokawa, M.; Nakamizo, H.; Higuchi, H.; Adachi, M.; Nakatsumi, R. C.; Suzuki, H.; Saito, Hidetsugu; Ishii, H.

In: Clinical and Experimental Immunology, Vol. 129, No. 1, 2002, p. 86-91.

Research output: Contribution to journalArticle

Kishikawa, H, Miura, S, Yoshida, H, Hirokawa, M, Nakamizo, H, Higuchi, H, Adachi, M, Nakatsumi, RC, Suzuki, H, Saito, H & Ishii, H 2002, 'Transmural pressure induces IL-6 secretion by intestinal epithelial cells', Clinical and Experimental Immunology, vol. 129, no. 1, pp. 86-91. https://doi.org/10.1046/j.1365-2249.2002.01895.x
Kishikawa, H. ; Miura, S. ; Yoshida, H. ; Hirokawa, M. ; Nakamizo, H. ; Higuchi, H. ; Adachi, M. ; Nakatsumi, R. C. ; Suzuki, H. ; Saito, Hidetsugu ; Ishii, H. / Transmural pressure induces IL-6 secretion by intestinal epithelial cells. In: Clinical and Experimental Immunology. 2002 ; Vol. 129, No. 1. pp. 86-91.
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