Transplantation of galectin-1-expressing human neural stem cells into the injured spinal cord of adult common marmosets

Junichi Yamane, Masaya Nakamura, Akio Iwanami, Masanori Sakaguchi, Hiroyuki Katoh, Masayuki Yamada, Suketaka Momoshima, Sachiyo Miyao, Ken Ishii, Norikazu Tamaoki, Tatsuji Nomura, Hirotaka James Okano, Yonehiro Kanemura, Yoshiaki Toyama, Hideyuki Okano

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56 Citations (Scopus)

Abstract

Delayed transplantation of neural stem/progenitor cells (NS/PCs) into the injured spinal cord can promote functional recovery in adult rats and monkeys. To enhance the functional recovery after NS/PC transplantation, we focused on galectin-1, a carbohydrate-binding protein with pleiotropic roles in cell growth, differentiation, apoptosis, and neurite outgrowth. Here, to determine the combined therapeutic effect of NS/PC transplantation and galectin-1 on spinal cord injury (SCI), human NS/PCs were transfected by lentivirus with galectin-1 and green fluorescent protein (GFP), (Gal-NS/PCs) or GFP alone (GFP-NS/PCs), expanded in vitro, and then transplanted into the spinal cord of adult common marmosets, 9 days after contusive cervical SCI. The animals' motor function was evaluated by their spontaneous motor activity, bar grip power, and performance on a treadmill test. Histological analyses revealed that the grafted human NS/PCs survived and differentiated into neurons, astrocytes, and oligodendrocytes. There were significant differences in the myelinated area, corticospinal fibers, and serotonergic fibers among the Gal-NS/PC, GFP-NS/PC, vehicle-control, and sham-operated groups. The Gal-NS/PC-grafted animals showed a better performance on all the behavioral tests compared with the other groups. These findings suggest that Gal-NS/PCs have better therapeutic potential than NS/PCs for SCI in nonhuman primates and that human Gal-NS/PC transplantation might be a feasible treatment for human SCI.

Original languageEnglish
Pages (from-to)1394-1405
Number of pages12
JournalJournal of neuroscience research
Volume88
Issue number7
DOIs
Publication statusPublished - 2010 May 15

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Keywords

  • Common marmoset
  • Galectin-1
  • Neural stem/progenitor cells
  • Preclinical study
  • Spinal cord injury

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Yamane, J., Nakamura, M., Iwanami, A., Sakaguchi, M., Katoh, H., Yamada, M., Momoshima, S., Miyao, S., Ishii, K., Tamaoki, N., Nomura, T., Okano, H. J., Kanemura, Y., Toyama, Y., & Okano, H. (2010). Transplantation of galectin-1-expressing human neural stem cells into the injured spinal cord of adult common marmosets. Journal of neuroscience research, 88(7), 1394-1405. https://doi.org/10.1002/jnr.22322