Transplantation of in vitro-expanded fetal neural progenitor cells results in neurogenesis and functional recovery after spinal cord contusion injury in adult rats

Y. Ogawa, K. Sawamoto, T. Miyata, S. Miyao, M. Watanabe, Masaya Nakamura, B. S. Bregman, M. Koike, Y. Uchiyama, Y. Toyama, Hideyuki Okano

Research output: Contribution to journalArticle

396 Citations (Scopus)

Abstract

Neural progenitor cells, including neural stem cells, are a potential expandable source of graft material for transplantation aimed at repairing the damaged CNS. Here we present the first evidence that in vitro-expanded fetus-derived neurosphere cells were able to generate neurons in vivo and improve motor function upon transplantation into an adult rat spinal-cord-contusion injury model. As the source of graft material, we used a neural stem cell-enriched population that was derived from rat embryonic spinal cord (E14.5) and expanded in vitro by neurosphere formation. Nine days after contusion injury, these neurosphere cells were transplanted into adult rat spinal cord at the injury site. Histological analysis 5 weeks after the transplantation showed that mitotic neurogenesis occurred from the transplanted donor progenitor cells within the adult rat spinal cord, a nonneurogenic region; that these donor-derived neurons extended their processes into the host tissues; and that the neurites formed synaptic structures. Furthermore, analysis of motor behavior using a skilled reaching task indicated that the treated rats showed functional recovery. These results indicate that in vitro-expanded neurosphere cells derived from the fetal spinal cord are a potential source for transplantable material for treatment of spinal cord injury.

Original languageEnglish
Pages (from-to)925-933
Number of pages9
JournalJournal of Neuroscience Research
Volume69
Issue number6
DOIs
Publication statusPublished - 2002 Sep 15

Fingerprint

Neurogenesis
Spinal Cord Injuries
Stem Cells
Transplantation
Spinal Cord
Neural Stem Cells
Transplants
Neurons
Contusions
Neurites
Fetus
In Vitro Techniques
Wounds and Injuries
Population

Keywords

  • Neural stem cells
  • Regeneration
  • Therapeutic use

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Transplantation of in vitro-expanded fetal neural progenitor cells results in neurogenesis and functional recovery after spinal cord contusion injury in adult rats. / Ogawa, Y.; Sawamoto, K.; Miyata, T.; Miyao, S.; Watanabe, M.; Nakamura, Masaya; Bregman, B. S.; Koike, M.; Uchiyama, Y.; Toyama, Y.; Okano, Hideyuki.

In: Journal of Neuroscience Research, Vol. 69, No. 6, 15.09.2002, p. 925-933.

Research output: Contribution to journalArticle

Ogawa, Y. ; Sawamoto, K. ; Miyata, T. ; Miyao, S. ; Watanabe, M. ; Nakamura, Masaya ; Bregman, B. S. ; Koike, M. ; Uchiyama, Y. ; Toyama, Y. ; Okano, Hideyuki. / Transplantation of in vitro-expanded fetal neural progenitor cells results in neurogenesis and functional recovery after spinal cord contusion injury in adult rats. In: Journal of Neuroscience Research. 2002 ; Vol. 69, No. 6. pp. 925-933.
@article{3803c248b97245238aab481b17d41f89,
title = "Transplantation of in vitro-expanded fetal neural progenitor cells results in neurogenesis and functional recovery after spinal cord contusion injury in adult rats",
abstract = "Neural progenitor cells, including neural stem cells, are a potential expandable source of graft material for transplantation aimed at repairing the damaged CNS. Here we present the first evidence that in vitro-expanded fetus-derived neurosphere cells were able to generate neurons in vivo and improve motor function upon transplantation into an adult rat spinal-cord-contusion injury model. As the source of graft material, we used a neural stem cell-enriched population that was derived from rat embryonic spinal cord (E14.5) and expanded in vitro by neurosphere formation. Nine days after contusion injury, these neurosphere cells were transplanted into adult rat spinal cord at the injury site. Histological analysis 5 weeks after the transplantation showed that mitotic neurogenesis occurred from the transplanted donor progenitor cells within the adult rat spinal cord, a nonneurogenic region; that these donor-derived neurons extended their processes into the host tissues; and that the neurites formed synaptic structures. Furthermore, analysis of motor behavior using a skilled reaching task indicated that the treated rats showed functional recovery. These results indicate that in vitro-expanded neurosphere cells derived from the fetal spinal cord are a potential source for transplantable material for treatment of spinal cord injury.",
keywords = "Neural stem cells, Regeneration, Therapeutic use",
author = "Y. Ogawa and K. Sawamoto and T. Miyata and S. Miyao and M. Watanabe and Masaya Nakamura and Bregman, {B. S.} and M. Koike and Y. Uchiyama and Y. Toyama and Hideyuki Okano",
year = "2002",
month = "9",
day = "15",
doi = "10.1002/jnr.10341",
language = "English",
volume = "69",
pages = "925--933",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Transplantation of in vitro-expanded fetal neural progenitor cells results in neurogenesis and functional recovery after spinal cord contusion injury in adult rats

AU - Ogawa, Y.

AU - Sawamoto, K.

AU - Miyata, T.

AU - Miyao, S.

AU - Watanabe, M.

AU - Nakamura, Masaya

AU - Bregman, B. S.

AU - Koike, M.

AU - Uchiyama, Y.

AU - Toyama, Y.

AU - Okano, Hideyuki

PY - 2002/9/15

Y1 - 2002/9/15

N2 - Neural progenitor cells, including neural stem cells, are a potential expandable source of graft material for transplantation aimed at repairing the damaged CNS. Here we present the first evidence that in vitro-expanded fetus-derived neurosphere cells were able to generate neurons in vivo and improve motor function upon transplantation into an adult rat spinal-cord-contusion injury model. As the source of graft material, we used a neural stem cell-enriched population that was derived from rat embryonic spinal cord (E14.5) and expanded in vitro by neurosphere formation. Nine days after contusion injury, these neurosphere cells were transplanted into adult rat spinal cord at the injury site. Histological analysis 5 weeks after the transplantation showed that mitotic neurogenesis occurred from the transplanted donor progenitor cells within the adult rat spinal cord, a nonneurogenic region; that these donor-derived neurons extended their processes into the host tissues; and that the neurites formed synaptic structures. Furthermore, analysis of motor behavior using a skilled reaching task indicated that the treated rats showed functional recovery. These results indicate that in vitro-expanded neurosphere cells derived from the fetal spinal cord are a potential source for transplantable material for treatment of spinal cord injury.

AB - Neural progenitor cells, including neural stem cells, are a potential expandable source of graft material for transplantation aimed at repairing the damaged CNS. Here we present the first evidence that in vitro-expanded fetus-derived neurosphere cells were able to generate neurons in vivo and improve motor function upon transplantation into an adult rat spinal-cord-contusion injury model. As the source of graft material, we used a neural stem cell-enriched population that was derived from rat embryonic spinal cord (E14.5) and expanded in vitro by neurosphere formation. Nine days after contusion injury, these neurosphere cells were transplanted into adult rat spinal cord at the injury site. Histological analysis 5 weeks after the transplantation showed that mitotic neurogenesis occurred from the transplanted donor progenitor cells within the adult rat spinal cord, a nonneurogenic region; that these donor-derived neurons extended their processes into the host tissues; and that the neurites formed synaptic structures. Furthermore, analysis of motor behavior using a skilled reaching task indicated that the treated rats showed functional recovery. These results indicate that in vitro-expanded neurosphere cells derived from the fetal spinal cord are a potential source for transplantable material for treatment of spinal cord injury.

KW - Neural stem cells

KW - Regeneration

KW - Therapeutic use

UR - http://www.scopus.com/inward/record.url?scp=0037106211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037106211&partnerID=8YFLogxK

U2 - 10.1002/jnr.10341

DO - 10.1002/jnr.10341

M3 - Article

C2 - 12205685

AN - SCOPUS:0037106211

VL - 69

SP - 925

EP - 933

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 6

ER -