Transplantation of mesenchymal stem cells attenuates myocardial injury and dysfunction in a rat model of acute myocarditis

Shunsuke Ohnishi, Bobby Yanagawa, Koichi Tanaka, Yoshinori Miyahara, Hiroaki Obata, Masaharu Kataoka, Makoto Kodama, Hatsue Ishibashi-Ueda, Kenji Kangawa, Soichiro Kitamura, Noritoshi Nagaya

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

Acute myocarditis is a non-ischemic inflammatory disease of the myocardium for which there is currently no specific treatment. We have previously shown that mesenchymal stem cells (MSC) can ameliorate heart injury during acute ischemia and in dilated cardiomyopathy; however, the therapeutic potential in acute myocarditis is unclear. In this study, we investigated the ability of MSC to attenuate myocardial injury and dysfunction during the acute phase of experimental myocarditis. Ten-week-old male Lewis rats were injected with porcine myosin to induce myocarditis. Cultured MSC (3 × 106 cells/rat) were injected intravenously 7 days after myosin injection. At 3 weeks, myosin injection resulted in severe inflammation and significant deterioration of cardiac function. MSC transplantation attenuated increases in CD68-positive inflammatory cells and monocyte chemoattractant protein-1 (MCP-1) expression in myocardium, and improved cardiac function in this model. Furthermore, myocardial capillary density was higher in myocarditis tissue, and was further increased by MSC transplantation. In vitro, cultured adult rat cardiomyocytes were injured in response to MCP-1, whereas this effect was attenuated by MSC-derived conditioned medium, suggesting cardioprotective effects of MSC acting in a paracrine manner. MSC transplantation attenuated myocardial injury and dysfunction in a rat model of acute myocarditis, at least in part through paracrine effects of MSC.

Original languageEnglish
Pages (from-to)88-97
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume42
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1
Externally publishedYes

Fingerprint

Mesenchymal Stem Cell Transplantation
Myocarditis
Mesenchymal Stromal Cells
Wounds and Injuries
Myosins
Chemokine CCL2
Myocardium
Heart Injuries
Injections
Dilated Cardiomyopathy
Conditioned Culture Medium
Cardiac Myocytes
Swine
Ischemia
Inflammation
Therapeutics

Keywords

  • Acute myocarditis
  • Cell death
  • Cytokine
  • Mesenchymal stem cell
  • Paracrine effect

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Transplantation of mesenchymal stem cells attenuates myocardial injury and dysfunction in a rat model of acute myocarditis. / Ohnishi, Shunsuke; Yanagawa, Bobby; Tanaka, Koichi; Miyahara, Yoshinori; Obata, Hiroaki; Kataoka, Masaharu; Kodama, Makoto; Ishibashi-Ueda, Hatsue; Kangawa, Kenji; Kitamura, Soichiro; Nagaya, Noritoshi.

In: Journal of Molecular and Cellular Cardiology, Vol. 42, No. 1, 01.01.2007, p. 88-97.

Research output: Contribution to journalArticle

Ohnishi, S, Yanagawa, B, Tanaka, K, Miyahara, Y, Obata, H, Kataoka, M, Kodama, M, Ishibashi-Ueda, H, Kangawa, K, Kitamura, S & Nagaya, N 2007, 'Transplantation of mesenchymal stem cells attenuates myocardial injury and dysfunction in a rat model of acute myocarditis', Journal of Molecular and Cellular Cardiology, vol. 42, no. 1, pp. 88-97. https://doi.org/10.1016/j.yjmcc.2006.10.003
Ohnishi, Shunsuke ; Yanagawa, Bobby ; Tanaka, Koichi ; Miyahara, Yoshinori ; Obata, Hiroaki ; Kataoka, Masaharu ; Kodama, Makoto ; Ishibashi-Ueda, Hatsue ; Kangawa, Kenji ; Kitamura, Soichiro ; Nagaya, Noritoshi. / Transplantation of mesenchymal stem cells attenuates myocardial injury and dysfunction in a rat model of acute myocarditis. In: Journal of Molecular and Cellular Cardiology. 2007 ; Vol. 42, No. 1. pp. 88-97.
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