Transplantation of neural stem cells for spinal cord injury

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Neural progenitor cells, including neural stem cells (NSCs), are an important potential graft material for cell therapeutics of damaged spinal cord. Here we used as a source of graft material a NSC-enriched population derived from human fetal spinal cord (Embryonic week 8-9) and expanded in vitro by neurosphere formation. NSCs labeled with BrdU (TP) or culture medium (CON) were transplanted into the adult marmoset spinal cord after contusion injury at C5 level. Grafted NSCs survived and migrated up to 7 mm far from the lesion epicenter. Double-staining with TuJ1 for neuron, GFAP for astrocyte, or CNPase for oligodendrocyte and BrdU revealed that grafted NSCs differentiated into neurons and oligodendrocytes 8 weeks after transplantation. More neurofilaments were observed in TP than those of CON. Furthermore, behavioral assessment of forelimb muscle strength using bar grip test and amount of spontaneous motor activity using infrared-rays monitoring revealed that the grafted NSCs significantly increased both of them compared to those of CON. These results indicate that in vitro expanded NSCs derived from human fetal spinal cord are useful sources for the therapeutics of spinal cord injury in primates.

Original languageEnglish
Pages (from-to)874-876
Number of pages3
JournalClinical Neurology
Volume45
Issue number11
Publication statusPublished - 2005 Nov

Fingerprint

Neural Stem Cells
Spinal Cord Injuries
Transplantation
Spinal Cord
Oligodendroglia
Bromodeoxyuridine
2',3'-Cyclic-Nucleotide Phosphodiesterases
Infrared Rays
Transplants
Neurons
Callithrix
Intermediate Filaments
Forelimb
Muscle Strength
Hand Strength
Astrocytes
Primates
Culture Media
Motor Activity
Stem Cells

Keywords

  • Neural stem cell
  • Regeneration
  • Spinal cord injury
  • Transplantation

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Transplantation of neural stem cells for spinal cord injury. / Nakamura, Masaya; Toyama, Yoshiaki; Okano, Hideyuki.

In: Clinical Neurology, Vol. 45, No. 11, 11.2005, p. 874-876.

Research output: Contribution to journalArticle

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