Transporter mRNA expression in a conditionally immortalized rat small intestine epithelial cell line (TR-SIE).

Ken ichi Hosoya, Masatoshi Tomi, Megumi Takayama, Yuko Komokata, Daisuke Nakai, Taro Tokui, Kenji Nishimura, Masatsugu Ueda, Masuo Obinata, Satoko Hori, Sumio Ohtsuki, Gordon L. Amidon, Tetsuya Terasaki

Research output: Contribution to journalArticle

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Abstract

Small intestine epithelial cell lines (TR-SIE), which are established from the small intestine of transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene (tsA58 Tg rat), were used to characterize the mRNA expression of small intestine transporters. TR-SIE cells had a polygonal morphology and expressed cytokeratin protein and villin mRNA. Although the large T-antigen was strongly expressed at 33 degrees C, this was reduced at 37 and 39 degrees C. Concomitantly, the cell growth was arrested at 37 and 39 degrees C compared with that at 33 degrees C, suggesting that TR-SIE cells are conditionally immortalized cell lines. RT-PCR analysis revealed that TR-SIE cells expressed ABCB1 (mdr1a and mdr1b), ABCB4 (mdr2), ABCC2 (mrp2), ABCC6 (mrp6), ABCG1, ABCG2 (bcrp/mxr), Slc21a7 (Oatp3), Slc15a1 (PepT1), and Slc16a1 (Mct1). Conditionally immortalized rat small intestine epithelial cell lines were established from tsA58 Tg rats and expressed the mRNA of intestinal transporters.

Original languageEnglish
Pages (from-to)264-269
Number of pages6
JournalDrug Metabolism and Pharmacokinetics
Volume19
Issue number4
Publication statusPublished - 2004
Externally publishedYes

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Small Intestine
Epithelial Cells
Cell Line
Messenger RNA
Viral Tumor Antigens
Transgenic Rats
Simian virus 40
Keratins
Polymerase Chain Reaction
Temperature
Growth
Genes
Proteins

Cite this

Hosoya, K. I., Tomi, M., Takayama, M., Komokata, Y., Nakai, D., Tokui, T., ... Terasaki, T. (2004). Transporter mRNA expression in a conditionally immortalized rat small intestine epithelial cell line (TR-SIE). Drug Metabolism and Pharmacokinetics, 19(4), 264-269.

Transporter mRNA expression in a conditionally immortalized rat small intestine epithelial cell line (TR-SIE). / Hosoya, Ken ichi; Tomi, Masatoshi; Takayama, Megumi; Komokata, Yuko; Nakai, Daisuke; Tokui, Taro; Nishimura, Kenji; Ueda, Masatsugu; Obinata, Masuo; Hori, Satoko; Ohtsuki, Sumio; Amidon, Gordon L.; Terasaki, Tetsuya.

In: Drug Metabolism and Pharmacokinetics, Vol. 19, No. 4, 2004, p. 264-269.

Research output: Contribution to journalArticle

Hosoya, KI, Tomi, M, Takayama, M, Komokata, Y, Nakai, D, Tokui, T, Nishimura, K, Ueda, M, Obinata, M, Hori, S, Ohtsuki, S, Amidon, GL & Terasaki, T 2004, 'Transporter mRNA expression in a conditionally immortalized rat small intestine epithelial cell line (TR-SIE).', Drug Metabolism and Pharmacokinetics, vol. 19, no. 4, pp. 264-269.
Hosoya, Ken ichi ; Tomi, Masatoshi ; Takayama, Megumi ; Komokata, Yuko ; Nakai, Daisuke ; Tokui, Taro ; Nishimura, Kenji ; Ueda, Masatsugu ; Obinata, Masuo ; Hori, Satoko ; Ohtsuki, Sumio ; Amidon, Gordon L. ; Terasaki, Tetsuya. / Transporter mRNA expression in a conditionally immortalized rat small intestine epithelial cell line (TR-SIE). In: Drug Metabolism and Pharmacokinetics. 2004 ; Vol. 19, No. 4. pp. 264-269.
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