Transporters on a chip: A fluorescence analysis of an atp-binding cassette (ABC)-transporter

H. Sasaki, H. Onoe, T. Osaki, R. Kawano, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We have developed a fluorescence-based microfluidic system for high-throughput analyses of the function of ATP-binding cassette (ABC) transporters. Our device consists of polydimethylsiloxane (PDMS) and a glass that tethers vesicles on its surface. Although PDMS is a great material for fabrication of microfluidic channels, it absorbs fluorescent molecules and hampers quantitive fluorescence analyses. We found that conformal deposition of parylenes on the inner surface of PDMS channels dramatically suppressed the absorption of a fluorescent dye, Rhodamine B. In the parylene-coated PDMS channels, immobilized ABC-transporter (MDR1) vesicles exhibited an ATP-dependent transport of a fluorescent substrate, Rhodamine 123.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages842-844
Number of pages3
Publication statusPublished - 2010 Dec 1
Externally publishedYes
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/10/310/10/7

Keywords

  • Abc transporter
  • Fluorescence
  • Microfluidic channel
  • Parylene

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Sasaki, H., Onoe, H., Osaki, T., Kawano, R., & Takeuchi, S. (2010). Transporters on a chip: A fluorescence analysis of an atp-binding cassette (ABC)-transporter. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 842-844). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).