TY - GEN
T1 - Transporters on a chip
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
AU - Sasaki, H.
AU - Onoe, H.
AU - Osaki, T.
AU - Kawano, R.
AU - Takeuchi, S.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - We have developed a fluorescence-based microfluidic system for high-throughput analyses of the function of ATP-binding cassette (ABC) transporters. Our device consists of polydimethylsiloxane (PDMS) and a glass that tethers vesicles on its surface. Although PDMS is a great material for fabrication of microfluidic channels, it absorbs fluorescent molecules and hampers quantitive fluorescence analyses. We found that conformal deposition of parylenes on the inner surface of PDMS channels dramatically suppressed the absorption of a fluorescent dye, Rhodamine B. In the parylene-coated PDMS channels, immobilized ABC-transporter (MDR1) vesicles exhibited an ATP-dependent transport of a fluorescent substrate, Rhodamine 123.
AB - We have developed a fluorescence-based microfluidic system for high-throughput analyses of the function of ATP-binding cassette (ABC) transporters. Our device consists of polydimethylsiloxane (PDMS) and a glass that tethers vesicles on its surface. Although PDMS is a great material for fabrication of microfluidic channels, it absorbs fluorescent molecules and hampers quantitive fluorescence analyses. We found that conformal deposition of parylenes on the inner surface of PDMS channels dramatically suppressed the absorption of a fluorescent dye, Rhodamine B. In the parylene-coated PDMS channels, immobilized ABC-transporter (MDR1) vesicles exhibited an ATP-dependent transport of a fluorescent substrate, Rhodamine 123.
KW - Abc transporter
KW - Fluorescence
KW - Microfluidic channel
KW - Parylene
UR - http://www.scopus.com/inward/record.url?scp=84883754961&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883754961&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883754961
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 842
EP - 844
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -